Expression Profile of Long Noncoding RNAs in Human Earlobe Keloids: A Microarray Analysis

Guo, Liang; Xu, Kai; Yan, Hongbo; Feng, Haifeng; Chai, Limin; Xu, Guohai

doi:10.1155/2016/5893481

Cited by 23 publications

(23 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once the basic knowledge of the lncRNA was recognized, its application followed. For application studies, the lncRNA has the potential for diagnosis as a biomarker [ 29 ]. It was recommended that lncRNA might be a treatment target in the future.…”

Section: Discussionmentioning

confidence: 99%

Bibliometric Analysis of Global Scientific Research on lncRNA: A Swiftly Expanding Trend

Zhai

Zhao

Wang

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

To investigate trends in long-noncoding (lnc) RNA research systematically, we compared the contribution of publications among different regions, institutions, and authors. Publications on lncRNA were retrieved from Web of Science (WoS) from 1975 to 2017. A total of 3879 papers were identified, and together they were cited 62967 times. The literature on lncRNA had been continuously growing since 2006, and the expansion might continue at a rapid pace until around 2021. China contributed the greatest proportion (63.47%) of lncRNA publications, and the USA ranked second in the number of publications (944 articles), while it had the highest citation frequency (43168 times) and H-index (97). The journal Oncotarget has the greatest number of publications on lncRNA research, with 305 papers. The keywords could be stratified into two clusters: cluster 1 (application) and cluster 2 (characteristics). Correspondingly, the “TNM stage,” “epithelial mesenchymal transition (EMT),” “cell apoptosis,” and “overall survival” are research hotspots since 2015. Thus, research on lncRNA showed a swiftly expanding trend, with China making the largest contribution. The focus on lncRNA is gradually shifting from “characteristics” to “application.”

show abstract

Section: Discussionmentioning

confidence: 99%

Bibliometric Analysis of Global Scientific Research on lncRNA: A Swiftly Expanding Trend

Zhai

Zhao

Wang

et al. 2018

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Microarray profiling of long noncoding RNAs (lncRNAs) has also been performed in lesional keloid tissue, identifying altered expression of CACNA1G-AS1 and HNF1A-AS1, which interact not only with pro-fibrotic TGF-β pathways, but also with cellular adhesion and tight junction signaling [ 40 , 41 ]. Another study reported altered expressions of 30 lncRNAs relating to hedgehog signaling pathways, which were accompanied by mRNA changes reflecting upregulation of cell proliferation and tissue repair and downregulation of apoptotic pathways in keloid tissue [ 42 ].…”

Section: Fibrosing Disorders Of the Skinmentioning

confidence: 99%

Genomics of Human Fibrotic Diseases: Disordered Wound Healing Response

Stone

Chen

Burgess

et al. 2020

IJMS

View full text Add to dashboard Cite

Fibrotic disease, which is implicated in almost half of all deaths worldwide, is the result of an uncontrolled wound healing response to injury in which tissue is replaced by deposition of excess extracellular matrix, leading to fibrosis and loss of organ function. A plethora of genome-wide association studies, microarrays, exome sequencing studies, DNA methylation arrays, next-generation sequencing, and profiling of noncoding RNAs have been performed in patient-derived fibrotic tissue, with the shared goal of utilizing genomics to identify the transcriptional networks and biological pathways underlying the development of fibrotic diseases. In this review, we discuss fibrosing disorders of the skin, liver, kidney, lung, and heart, systematically (1) characterizing the initial acute injury that drives unresolved inflammation, (2) identifying genomic studies that have defined the pathologic gene changes leading to excess matrix deposition and fibrogenesis, and (3) summarizing therapies targeting pro-fibrotic genes and networks identified in the genomic studies. Ultimately, successful bench-to-bedside translation of observations from genomic studies will result in the development of novel anti-fibrotic therapeutics that improve functional quality of life for patients and decrease mortality from fibrotic diseases.

show abstract

“…More and more studies showed that lncRNAs play an important role in different biological processes, such as cell growth, proliferation, and apoptosis [7]. It has been proposed that plenty of lncRNAs were closely related to the progression of keloid fibrosis [8], such as HNF1A-AS1 [9], COL1A2-AS1 [10], CACNA1G-AS1, HOXA11-AS, LINC00312, and RP11-91I11.1 [11]. Recent study demonstrated that calcium voltage-gated channel subunit alpha1 G antisense RNA 1 (CACNA1G-AS1) was up-regulated in keloid and had a positive effect on cell migration in keloid fibrosis [12].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA CACNA1G-AS1 promotes proliferation and invasion and inhibits apoptosis by regulating expression of miR-205 in human keloid fibroblasts

et al. 2020

View full text Add to dashboard Cite

Background: Keloid is a fibrous tissue proliferative disease in which proliferative scars grow beyond the boundary of the original wound skin. Long non-coding RNAs (lncRNAs), as competing endogenous RNAs (ceRNAs), bind to microRNAs (miRNAs) to regulate various biological processes. The present study was aim to illuminate the mechanism of calcium voltage-gated channel subunit alpha1 G antisense RNA 1 (CACNA1G-AS1) in human keloid fibroblasts. Methods: CACNA1G-AS1 and miR-205 levels were detected using quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) assay was used to measure the proliferation and transwell assay was performed to evaluate cell invasion. Furthermore, the apoptosis rates of cells were evaluated by flow cytometry analysis, and the activity of caspase-3 in keloid fibroblasts was tested by Caspase-3 activity assay. Dual luciferase reporter assay was carried out to examine the relationship between CACNA1G-AS1 and miR-205 and RNA immunoprecipitation (RIP) assay was conducted to further confirm the relation. Results: CACNA1G-AS1 level was up-regulated in keloid tissues and keloid fibroblasts. CACNA1G-AS1 overexpression promoted proliferation and invasion and suppressed apoptosis of keloid fibroblasts. Moreover, miR-205 was targeted by CACNA1G-AS1 and miR-205 was markedly decreased in keloid tissues and keloid fibroblasts. Also, miR-205 expression was negatively regulated by CACNA1G-AS1 and miR-205 silencing enhanced proliferation and invasion and inhibited apoptosis. Furthermore, CACNA1G-AS1 and miR-205 played the antagonistic role in miR-205 expression, proliferation, invasion, and apoptosis of keloid fibroblasts. Conclusion: CACNA1G-AS1 suppressed miR-205 expression to promote proliferation and invasion and inhibit apoptosis in human keloid fibroblasts.

show abstract

Expression Profile of Long Noncoding RNAs in Human Earlobe Keloids: A Microarray Analysis

Cited by 23 publications

References 28 publications

Bibliometric Analysis of Global Scientific Research on lncRNA: A Swiftly Expanding Trend

Bibliometric Analysis of Global Scientific Research on lncRNA: A Swiftly Expanding Trend

Genomics of Human Fibrotic Diseases: Disordered Wound Healing Response

Long non-coding RNA CACNA1G-AS1 promotes proliferation and invasion and inhibits apoptosis by regulating expression of miR-205 in human keloid fibroblasts

Contact Info

Product

Resources

About