Expression profile of HSP genes during different seasons in goats (Capra hircus)

Abstract

The present study has demonstrated the expression of HSP60, HSP70, HSP90, and UBQ in peripheral blood mononuclear cells (PBMCs) during different seasons in three different age groups (Groups I, II, and III with age of 0-2, 2-5, and >5 years, respectively) of goats of tropical and temperate regions. Real-time polymerase chain reaction was applied to investigate mRNA expression of examined factors. Specificity of the desired products was documented using analysis of the melting temperature and high-resolution gel electrophoresis to verify that the transcripts are of the exact molecular size predicted. The mRNA expression of HSP60, HSP90, and UBQ was significantly higher (P < 0.05) in all age groups during peak summer season as compared with peak winter season in both tropical and temperate region goats. HSP70 mRNA expression was significantly higher (P < 0.05) during summer season as compared with winter season in tropical region goats. However, in the temperate region, in goats from all the three age groups studied, a non-significant difference of HSP70 expression between summer and winter seasons was noticed. In conclusion, results demonstrate that (1) HSP genes are expressed in caprine PBMCs and (2) higher expression of HSPs during thermal stress suggest possible involvement of them to ameliorate deleterious effect of thermal stress so as to maintain cellular integrity and homeostasis in goats.

A comparative study on the expression profile of MCTs and HSPs in Ghungroo and Large White Yorkshire breeds of pigs during different seasons

“…In the present study, we observed that HSP70 mRNA expression was found to be significantly higher (P<0.05) during the summer in both breeds in comparison with the winter season. Our findings are in accordance with previous studies in which heat stress-induced HSP70 expression was observed in the goat PBMCs (Banerjee et al 2014;Dangi et al 2012) and bovine lymphocytes (Lacetera et al 2006;Upadhyay 2007, 2010). During the winter, HSP70 expression was approximately three times higher in Ghungroo compared to LWY.…”

“…Its major functions are protein folding, cytoprotection, and as molecular chaperones (Dangi et al 2012). In the present study, we observed that HSP70 mRNA expression was found to be significantly higher (P<0.05) during the summer in both breeds in comparison with the winter season.…”

“…In Ghungroo, the increased HSP27 expression in the colon during winter may be due to the fact that HSP27 was reported to play a major role in protecting the intestinal epithelial cell from damage by upregulating its expression (Liu et al 2012). The variation in HSP expression due to breed difference was also reported in other species (Dangi et al 2012;Banerjee et al 2014) and HSPs expression also acts as a potential indicator of animal adaptation to harsh environmental stress (Valenzuela 2000;Hansen 2004). …”

“…The major functions are protein translocation and regulation of steroid hormone receptors (Dangi et al 2012). Our present study found that the mRNA expression of HSP90 in both the breeds during the summer season was significantly higher (P<0.05) than the corresponding values in thigh muscle during the winter season.…”

Seasonal effects on plasma cortisol concentrations in the Bedouin buck: circadian studies and response to ACTH

“…plasma cortisol levels, but acute exposure to high temperatures (in the range of 40°C as observed during summer in the present experiments) increases plasma cortisol levels and triggers other stress responses such as the expression of HSP in PBMC (Dangi et al, 2012;Sharma et al, 2013). These non-linear effects of increased temperature on HPA axis activity can explain apparently divergent experimental results of the effects of seasons, depending on the range of temperatures encountered at each experimental location.…”