Expression profile changes of cytochrome P450 genes between thiamethoxam susceptible and resistant strains of Aphis gossypii Glover

Wu, Yongqiang; Xu, Hongfei; Pan, Yiou; Gao, Xiwu; Xi, Jinghui; Zhang, Juhong; Shang, Qingli

doi:10.1016/j.pestbp.2018.05.007

Cited by 57 publications

(43 citation statements)

References 32 publications

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“…The overexpression of the P450 monooxygenase enzyme is the most common mechanism of imidacloprid and chlorpyrifos resistance [23][24][25]. In aphids, P450 monooxygenase enzyme also plays an important role in insecticide detoxification and resistance [26][27][28]. In the present study, samples treated with chlorpyrifos showed five upregulated P450 unigenes, as well as a fold change in imidacloprid-treated samples.…”

Section: Discussionsupporting

confidence: 53%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 60–2267 differentially expressed unigenes (DEUs). Among these DEUs, 31–790 unigenes were classified into 66–786 categories of gene ontology (GO) functional groups, and 24–760 DEUs could be mapped into 54–268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids.

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Section: Discussionsupporting

confidence: 53%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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“…Delivery of CYP6AY1 and CYP6ER1 double‐stranded RNA (dsRNA) significantly enhanced the susceptibility of brown planthopper ( Nilaparvata lugens ) to imidacloprid . RNAi targeting CYP6CY14 significantly increased the sensitivity of resistant aphids to thiamethoxam . RNAi‐mediated silencing of CYP321B1 increased mortality by 25.6% and 38.9% when the fifth‐instar tobacco cutworm ( Spodoptera litura ) larvae were exposed to chlorpyrifos and β‐cypermethrin, respectively .…”

Section: Introductionmentioning

confidence: 99%

“…37 RNAi targeting CYP6CY14 significantly increased the sensitivity of resistant aphids to thiamethoxam. 38 RNAi-mediated silencing of CYP321B1 increased mortality by 25.6% and 38.9% when the fifth-instar tobacco cutworm (Spodoptera litura) larvae were exposed to chlorpyrifos and -cypermethrin, respectively. 39 Hence, in this study, RNAi was used to evaluate the role of P450 genes in insecticide detoxification in B. odoriphaga.…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Chen

Shan

Liu

et al. 2018

Pest Management Science

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“…High P450 enzyme activity levels were reported in neonicotinoid-resistant A. gossypii populations (Seyedebrahimi et al 2015, Shang et al 2012, Ulusoy et al 2018. It was stated that this situation is dependent on the changes in the expression levels of P450 genes, changes in catalytic activity or changes in substrate-specificity (Bergé et al 1998, Hirata et al 2017, Nikou et al 2003, Wu et al 2018. Various next-generation sequencing (NGS) studies have been carried out in relation to P450 expression levels (Hirata et al 2017, Wu et al 2018.…”

mentioning

confidence: 99%

“…It was stated that this situation is dependent on the changes in the expression levels of P450 genes, changes in catalytic activity or changes in substrate-specificity (Bergé et al 1998, Hirata et al 2017, Nikou et al 2003, Wu et al 2018. Various next-generation sequencing (NGS) studies have been carried out in relation to P450 expression levels (Hirata et al 2017, Wu et al 2018. Hirata et al (2017) reported 45 times higher activity levels in comparison to reference values in the CYP6CY13 and CYP6CY22 genes in 10 different genes in the cytochrome P450 gene family in thiamethoxam-resistant A. gossypii populations.…”

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confidence: 99%

Overexpression of cytochrome P450 genes in Aphis gossypii (Glover) in the cotton fields of the Çukurova region, Turkey

Ulusoy¹

2020

Bitki Koruma Bülteni

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Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), which is a polyphagous species, is among the main pests of the cotton plants in the whole world. The Çukurova region is an area where polyculture agriculture activities are carried out. While insecticides in the neonicotinoid group are prevalently used in the cotton fields in this region for controlling A. gossypii, the problem of resistance is frequently encountered. This study determined the relative expression levels of the CYP6CY22 and CYP6CY13 genes, cytochrome P450, acetylcholine esterase (AChE) and glutathione S-transferase (GST) enzyme activities in six different populations of A. gossypii collected in 2018 from cotton fields in the province of Adana in Southern Turkey. In comparison to the reference culture, there was an increase in gene regulation by 81.9 fold in the CYP6CY22 gene in the Hamitbey population and by 6 fold in the CYP6CY13 gene in the Gazipaşa population. Additionally, the Hamitbey population showed 4.3 times higher (0.74 U/ml) cytochrome P450 enzyme activity. The Çiftlikler population showed 5.9 fold higher AChE (0.82 U/ml) and 4.3 fold higher GST (2.68 U/ml) activities. The analyses revealed that the A. gossypii populations were exposed to high amounts of insecticides. Consequently, overexpressed genes, high levels of enzyme activities and metabolic resistance in based on cytochrome P450 activity were observed in the study. Cotton aphid, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), is one of the significant pests among aphids that reaches the economic loss threshold. A. gossypii is a polyphagous species that has a very broad spectrum of hosts. As in the entire world, they create harm on especially cotton and Cucurbitaceae species in Turkey (Ozgur and Sekeroglu

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Expression profile changes of cytochrome P450 genes between thiamethoxam susceptible and resistant strains of Aphis gossypii Glover

Cited by 57 publications

References 32 publications

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Identification of a novel cytochrome P450 CYP3356A1 linked with insecticide detoxification in Bradysia odoriphaga

Overexpression of cytochrome P450 genes in Aphis gossypii (Glover) in the cotton fields of the Çukurova region, Turkey

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