Expression, polymorphism and methylation pattern of interleukin-6 in periodontal tissues

Stefani, Florença Abdanur; Viana, Michelle Beatriz; Dupim, Ana Carolina; Brito, João Artur Ricieri; Gomez, Ricardo Santiago; Costa, José Eustáquio da; Moreira, Paula Rocha

doi:10.1016/j.imbio.2012.12.001

Cited by 56 publications

(55 citation statements)

References 53 publications

(57 reference statements)

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“…Among several kinds of cytokines, IL-6 has been shown to increase in the gingival crevicular fluids and in the gingival tissues of individuals with periodontitis (10). IL-6 is also a potent stimulator of osteoclast differentiation and bone resorption and an inhibitor of bone formation (2).…”

Section: Introductionmentioning

confidence: 99%

“…As the -174 G/C and -572 C/G polymorphisms of the IL-6 gene increase IL-6 expression, they may be associated with susceptibility to periodontitis (2,12,13). However, the frequency of many genetic alleles varies among ethnic groups, several studies have found contradictory results regarding the relationship between this gene and periodontitis (2,10,14). IL-10, an anti-inflammatory cytokine, plays a role in periodontitis by inhibiting synthesis of pro-inflammatory cytokines such as IL-1, -2, -6 and stimulating protective antibody production (15).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Toker¹,

Görgün²,

Korkmaz³

2017

Cent Eur J Public Health

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SUMMARYObjective: Pro-inflammatory cytokines, interleukin-6 (IL-6), demonstrated to be suppressed by interleukin-10 (IL-10) are known to be regulated by the transcription factor nuclear factor-κB(NF-κB). The aim of this study was to ascertain the association between genetic polymorphism of these genes (IL-6(-174), IL-10(-597) and NF-κB1-94ins/del)) and chronic/aggressive periodontitis.Methods: Forty-five patients with chronic periodontitis (CP), 58 patients with aggressive periodontitis (AP) and 38 periodontally healthy subjects were included in this study. Genomic DNA was isolated from whole blood samples. The NF-κB, IL-6, and IL-10 polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results: Among subjects for the ins/ins genotypes of NF-κB1 gene, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis group than in healthy controls (p = 0.023). A statistically significant difference in genotyping frequencies between AP group and healthy controls was observed for the IL-6 gene. The AA genotype of IL-10 was overrepresented in CP and AP groups compared to healthy controls (OR = 9.93, 95% CI: 2.11-46.7, OR = 5.7, 95% CI: 1.22-26.89, respectively).Conclusions: Within the limits of this study, it can be concluded that the IL-10 (-597) AA genotype is associated with susceptibility to chronic/ aggressive periodontitis and IL-6 (-174) GG genotypes and G allele seems to be associated with aggressive periodontitis.Clinical relevance: The results of the current study indicate that IL-6 and IL-10 genotypes seem to be associated with aggressive periodontitis. Also, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis subjects with carrying NF-κB1 ins/ins genotypes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Toker¹,

Görgün²,

Korkmaz³

2017

Cent Eur J Public Health

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“…After deleting the duplicates, record screen was performed. And 21 studies [5, 14–18, 21, 22, 24–26, 28–36, 38] involving 2422 CP patients and 3373 healthy individuals were identified to be eligible in present meta-analysis. The process of study selection and the reasons for exclusion are listed in Figure 1.…”

Section: Resultsmentioning

confidence: 99%

“…Of the 21 included studies, 10 studies were conducted in Caucasian population [5, 14, 17, 22, 28–30, 34–36], 6 studies were conducted in Brazilian population [15, 16, 21, 24, 26, 33], 4 studies were conducted in Asian population [18, 25, 32, 38], and 1 study was conducted in Indian population [31]. Seven studies involved nonsmokers as study subjects [5, 15, 22, 24–26, 31].…”

Section: Resultsmentioning

confidence: 99%

Interleukin-6-174G/C Polymorphism Contributes to Periodontitis Susceptibility: An Updated Meta-Analysis of 21 Case-Control Studies

Zhu

Guo

et al. 2016

Disease Markers

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Introduction. Chronic Periodontitis (CP) is suggested to be related to gene variations. Present study aims to quantitatively estimate the association between interleukin-6- (IL-6-) 174G/C polymorphism and CP susceptibility. Materials and Methods. Pubmed, Embase, and Web of Science were searched up to May 2016. The meta-analyses were performed using STATA 12.0. Results. 21 studies were yielded. Significant associations were found under heterozygote comparison and dominant model in studies fulfilling HWE (GC versus GG: OR = 0.690, 95% CI = 0.560–0.849, P = 0.000; CC + GC versus GG: OR = 0.690, 95% CI = 0.568–0.838, P < 0.001); significant associations were found under heterozygote comparison and dominant model in Caucasian studies fulfilling HWE (GC versus GG: OR = 0.752, 95% CI = 0.577–0.980, P = 0.035; CC + GC versus GG: OR = 0.737, 95% CI = 0.576–0.944, P = 0.016); significant associations were found under allele comparison, heterozygote comparison, and dominant model in Brazilian population (C versus G: OR = 0.648, 95% CI = 0.497–0.845, P = 0.001; GC versus GG: OR = 0.621, 95% CI = 0.441–0.876, P = 0.007; CC + GC versus GG: OR = 0.649, 95% CI = 0.470–0.896, P = 0.009). Conclusion. IL-6 174 polymorphism is associated with CP susceptibility. In Brazilian and Caucasian population, IL-6 174 GG genotype plays as a risk factor to CP.

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“…DNA methylation may be an important regulatory mechanism in controlling TNF-α transcriptional expression in periodontal disease [23]. Another study conducted by Stefani et.al evaluated DNA methylation of IL-6 in gingival biopsies of chronic periodontitis patients and controls, reported that there is no difference in methylation of IL-6 [24].…”

Section: Evidence For the Role Of Epigenetics In Periodontal Diseasementioning

confidence: 99%

Epigenetics - A Window Towards Personalized Periodontal Therapy

Abraham¹,

Saseendran²,

R³

2017

IOSR JDMS

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Expression, polymorphism and methylation pattern of interleukin-6 in periodontal tissues

Cited by 56 publications

References 53 publications

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Interleukin-6-174G/C Polymorphism Contributes to Periodontitis Susceptibility: An Updated Meta-Analysis of 21 Case-Control Studies

Epigenetics - A Window Towards Personalized Periodontal Therapy

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