Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)

Mahroof, Rizana; Zhu, Kun Yan; Neven, Lisa; Subramanyam, Bhadriraju; Bai, Jianfa

doi:10.1016/j.cbpb.2005.05.044

Cited by 120 publications

(69 citation statements)

References 47 publications

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“…Interestingly, Mahroof et al (2005) saw similar developmental changes in studies with the red flour beetle, Tribolium castaneum. In the red flour beetle, the lowest levels of a constitutively expressed HSP 70 gene were seen in pupae just prior to metamorphosis.…”

Section: Resultsmentioning

confidence: 67%

“…Interestingly, in a wide range of organisms, the expression of HSP 70 has also been shown to vary during development (Dix 1997;Feder and Hofmann 1999;Giudice et al 1999;Wood et al 1999;Yeh and Hsu 2002;Karouna-Renier et al 2003;Chang 2005;Mahroof et al 2005). The differential expression has been studied during events that involve morphological changes in organisms such as gametogenesis, embryogenesis, and metamorphosis (Feder and Hofmann 1999).…”

Section: Introductionmentioning

confidence: 99%

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Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)

Ueda

Boettcher

2009

Cell Stress and Chaperones

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For a variety of species, changes in the expression of heat shock proteins (HSP) have been linked to key developmental changes, i.e., gametogenesis, embryogenesis, and metamorphosis. Many marine invertebrates are known to have a biphasic life cycle where pelagic larvae go through settlement and metamorphosis as they transition to the benthic life stage. A series of experiments were run to examine the expression of heat shock protein 70 (HSP 70) during larval and early spat (initial benthic phase) development in the Eastern oyster, Crassostrea virginica. In addition, the impact of thermal stress on HSP 70 expression during these early stages was studied. C. virginica larvae and spat expressed three HSP 70 isoforms, two constitutive, HSC 77 and HSC 72, and one inducible, HSP 69. We found differences in the expression of both the constitutive and inducible forms of HSP 70 among larval and early juvenile stages and in response to thermal stress. Low expression of HSP 69 during early larval and spat development may be associated with the susceptibility of these stages to environmental stress. Although developmental regulation of HSP 70 expression has been widely recognized, changes in its expression during settlement and metamorphosis of marine invertebrates are still unknown. The results of the current study demonstrated a reduction of HSP 70 expression during settlement and metamorphosis in the Eastern oyster, C. virginica. Keywords

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Section: Resultsmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)

Ueda

Boettcher

2009

Cell Stress and Chaperones

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“…The difference in chlorpyrifosresistance was >100-fold between R R and S S . Goto & Kimura, 2004), Hymenoptera (Elekonich, 2009), Orthoptera (Qin et al, 2003, and Coleoptera (Mahroof et al, 2005;Dahlhoff & Rank, 2007). Interestingly, almost all eukaryotes have more than one gene that encodes Hsp70 proteins (Daugaard et al, 2007).…”

Section: Source Of Insectmentioning

confidence: 99%

“…According to subcellular location, eukaryotes possess four types of Hsp70s, each localized to a number of cellular compartments in the cell: cytoplasm, endoplasmic reticulum, mitochondrion, and chloroplast (Renner & Waters, 2007). Moreover, the cytoplasmic Hsp70s are classified into two kinds: one, heat shock inducible protein 70 (Hsp70), is expressed at low basal levels under non-stress conditions but can be quickly induced by heat shock and other environmental stresses; the other, heat shock cognate or constitutive protein 70 (Hsc70), is constitutively expressed under normal conditions and remains unchanged or slightly up-regulated upon exposure to stresses of one form or another (Mahroof et al, 2005;Daugaard et al, 2007). Although their expression patterns are different, both Hsp70 and Hsc70 participate in removing abnormal cellular proteins and help stabilize proteins during folding (Qin et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Identification of heat shock protein genes hsp70s and hsc70 and their associated mRNA expression under heat stress in insecticide-resistant and susceptible diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Zhang¹,

Wang²,

Jing³

et al. 2015

Eur. J. Entomol.

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Abstract.To gain further insight into the molecular features of the ubiquitous Hsp70 family of conserved heat shock proteins, total nine full-length cDNA sequences of inducible hsp70s and one constitutive hsc70 (Px-hsc70(C)) were isolated and characterized in the diamondback moth (DBM), Plutella xylostella, collected from Fuzhou, China. The nine Px-hsp70s cDNAs encoded the protein of between 629-669 amino acids with molecular weight ranging from 69.00-72.58 kDa and were derived from four hsp70 genes in the genome of DBM. The Px-hsc70(C) cDNA contained 1,953 bp of open reading frame (ORF), which produced a putative protein comprising 650 amino acids with a calculated molecular weight of 71.18 kDa. Whether in adults or larvae of chlorpyrifos-resistant (R R ) and chlorpyrifos-susceptible (S S ) strains of DBM, the basal level (at 25°C) of Px-hsc70(C) mRNA expression was high, but no significant up-regulation expression was found under heat stress. However, heat stress facilitated up-regulation expressions of Px-hsp70s, and S S DBM displayed higher up-regulation expression of Px-hsp70s than R R DBM. We suggest that higher up-regulation expression of Px-hsp70s in S S DBM is probably involved in their higher thermal tolerance.

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“…Conversely, HSC70 is actively expressed in normal cells, but does not change in response to stressors (Kiang & Tsokos 1998;Shim et al 2006). However, recent evidence indicates that the general pattern of hsp70/hsc70 gene expression is variable in response to different types and conditions of stressors and also in different stages during development (Lakhotia & Prasanth 2002;Lo et al 2004;Mahroof et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

Chen

Lou

et al. 2009

Biologia

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Heat shock protein 70 (HSP70) is an important member of the heat shock protein family. It plays a key role in the process of protecting cells by facilitating the folding of nascent peptides as well as the cellular stress response. We isolated and sequenced a full-length HSP70 cDNA from planarian Dugesia japonica (designated Djhsp70) using rapid amplification of cDNA ends (RACE). Djhsp70 cDNA is highly homologous to other reported hsp70 family genes, and the deduced amino acid sequence shares several eukaryotic HSP70 family motifs. The length of the Djhsp70 ORF from planarian genomic DNA and cDNA was identical, which indicated the absence of introns in the Djhsp70 gene. Semi-quantitative RT-PCR was employed to detect the expression levels of Djhsp70 in response to stressors. Our results indicated that Djhsp70 was an inducible gene, expressed in response to temperature changes, amputation and starvation. Interestingly, the phylogenetic analysis of DjHSP70 supports the idea that planarians belong to a new phylogenetic position -Lophotrochozoans. This is the first molecular analysis of a heat shock protein gene in planarians.

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Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)

Cited by 120 publications

References 47 publications

Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)

Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)

Identification of heat shock protein genes hsp70s and hsc70 and their associated mRNA expression under heat stress in insecticide-resistant and susceptible diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica

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