Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats

Yakan, Akın; Özkan, Hüseyin; Çamdeviren, Baran; Kaya, Ufuk; Karaaslan, İ̇rem; DALKIRAN, Sevda

doi:10.1038/s41598-021-88976-0

Cited by 7 publications

(9 citation statements)

References 57 publications

(44 reference statements)

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“…Pro-inflammatory cytokines released in infected mammary glands reduce the expression of FASN and ACACA [ 42 ]. Yakan et al [ 43 ] estimated a significant negative (r = − 0.407, p < 0.001) correlation between SCC and FASN as well. In the current study, some goats in a high SCC group with an average of 2201 ± 183×10 3 cells/mL could suffer from subclinical mastitis; thus, SCFA synthesis may have been disturbed in their udder parenchyma.…”

Section: Resultsmentioning

confidence: 99%

Associations between Somatic Cell Count and Milk Fatty Acid and Amino Acid Profile in Alpine and Saanen Goat Breeds

Šlyžius

Anskienė

Palubinskas

et al. 2023

Animals

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The main goal of this study was to evaluate the relation of different SCC levels in goat milk with goat milk yield, milk composition, FA, and AA profiles. Whereas the investigated herd was composed of Alpine and Saanen goats, the influence of breed on milk parameters and milk yield was also assessed. The research was carried out in 2022 at a Lithuanian dairy goat farm with 135 goats (Saanen = 66 and Alpine = 69) without evidence of clinical mastitis. The current research revealed a relationship between SCC with goat milk yield and composition. Goats with a high SCC had significantly lower milk yield (p < 0.001), lower content of lactose (p < 0.01), fat (p < 0.001) and higher protein content (p < 0.05) in their milk. The increase in most AA was significantly associated with increased SCC. The higher quantity of Asp, Glu, Ala, Met, His, Lys, Arg, EAA, NEAA, and TAA (compared with the low SCC group) (p < 0.05–0.01), Leu, Tyr, and BCAA (compared with the low and medium SCC group) were found in the milk of the high SCC group (p < 0.05–0.01). The distribution of the main FA groups was also related to SCC and showed a significant decrease in SCFA (p < 0.01–0.001) and an increase in LCFA, PUFA, and BCFA in the high SCC group (p < 0.05). All individual AA and their groups (EAA, NEAA, TAA, BCAA) were significantly lower in the milk of the Saanen goat breed (p < 0.001). The most individual FA ranged between goat breeds, while the total amount of SFA, UFA, and MUFA wasn’t affected by breed (p > 0.05). The research revealed a statistically significant relationship between SCC, AA, and FA, suggesting that these traits may be used as a biomarker in the goat selection process.

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Section: Resultsmentioning

confidence: 99%

Associations between Somatic Cell Count and Milk Fatty Acid and Amino Acid Profile in Alpine and Saanen Goat Breeds

Šlyžius

Anskienė

Palubinskas

et al. 2023

Animals

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“…Also, Ognik The oxidative state of the organism, like many other parameters, is under the control of molecular regulation.COX-2, is a gene controlled by more than 20 transcription factors and whose activity is increased due to conditions such as oxidative stress and inflammation (Luo et al, 2011).It has been reported that COX-2 in leukocytes increases in the level of mRNA and protein in the case of oxidative stress (Barbieri et al, 2003).NRF2 activity, on the other hand, increases the transcription levels of many antioxidant genes. When the increased activity of this transcription factor, which plays a primary role in the response to oxidative stress that may occur, is evaluated together with COX-2, it is understood that weaning causes the oxidative stress in kids (Yakan et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Higher ROS production in the blood activates antioxidant mechanism of the organism (Galarza et al, 2021). MDA,final products of lipid peroxidation, is major biomarker of oxidative stress (Yakan et al, 2021). The level of MDA is served as a reliable biomarker of lipid peroxidation (LPO) and usually served as a marker of LPO (Acaroz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

NRF2 is a potential biomarker for the evaluation of weaning-related oxidative stress in goat kids

Özkan

Kaya

Karaaslan

et al. 2023

J Hellenic Vet Med Soc

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Weaning stress is crucial threatening factor in animal breeding. The aim of this study was to investigate the effects of weaning to oxidative stress and stress status of goat kids at the molecular levels. In the study, blood samples were collected at the weaning process (7 days before weaning- Pre-W, Day of weaning- Day-W and 7 days after weaning- Post-W) from 24 healthy Damascus goats. Cortisol, MDA (Malondialdehyde), COX-2 (Cyclooxygenase-2), and NRF2 (Nuclear factor erythroid 2-related factor 2) proteins levels were investigated in plasma, while COX-2 and NRF2 genes expression levels were determined from leukocytes. Compared to Pre-W, COX-2 gene was upregulated almost 2-fold in Day-W. On the other hand, NRF2 gene expression levels were tended to upregulation in Day-W, and it was upregulated approximately 2-fold in Post-W. While MDA levels were gradually increased in the weaning process, the levels of COX-2 and NRF2 proteins showed similar changes with gene expression trends. Positive correlations were also found between cortisol and COX-2 and NRF-2 protein levels in plasma. It was thought that significant outputs gained in terms of MDA levels in plasma of kids at the weaning process. For the first time, determination of COX-2 and NRF2 levels both mRNA and protein levels in the weaning process of goat kids were satisfactorily evaluated in this study. Particularly, NRF2 was found to have the potential to be a fundamental biomarker to assess the oxidative status of weaning kids.

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“…Moreover, TAC and MDA concentrations have been reported to increase significantly over time in untreated cows with sub-clinical mastitis (Tabatabaee et al, 2021). Higher SCCs have also been strongly correlated with higher MDA levels (Yakan et al, 2021). Total antioxidant capacity can be used to provide a simple understanding of the antioxidant status in the body (Farghali et al, 2021;Abdel-Saeed and Salem, 2019).…”

Section: Total Oxidant Capacity (Toc) Total Antioxidant Capacity (Tac...mentioning

confidence: 99%

Relationship of Oxidative Stress and Some Blood Parameters with Quarters Affected by Mastitis in Dairy Cows

Akkuş¹,

Dinçer²,

Ekici³

et al. 2023

IJAR

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Background: This study was conducted to evaluate the relationship between oxidative stress and some blood parameters in different number of quarters affected with and without sub-clinical mastitis (SM). Methods: A total of 50 lactating Holstein dairy cows were used in the study. The study groups were as follows; Group 1 (negative test results and no SM), Group 2 (SM in one quarter), Group 3 (SM in two quarters), Group 4 (SM in three quarters) Group 5 (SM in four quarters). Milk samples were obtained from the infected quarters for both Somatic Cell Count (SCC) measurement and bacteriological growth/bacterial identification. Blood was collected for analysis of oxidative stress parameters (total oxidative capacity and total antioxidant capacity) and some blood parameters (glucose, total cholesterol, total bilirubin, aspartate aminotransferase [AST], alanine aminotransferase [ALT]). Result: Somatic Cell Count (SCC) was significantly lower in Group 1 than in Groups 2, 3, 4 and 5 (P less than 0.001). Glucose and total cholesterol levels were significantly higher in Group 5 than in Groups 1, 2, 3 and 4 (P less than 0.001). A significant difference was determined between all groups in respect of AST and ALT activities (P less than 0.001). The Total Oxidative Capacity (TOC) and oxidative stress index value (OSI) levels were significantly lower in Group 1 than in Groups 2, 3, 4 and 5 (P less than 0.001), while Total Antioxidant Capacity (TAC) levels were significantly higher (P less than 0.001). In conclusion, it was found that oxidative stress and blood-biochemistry values are significantly affected in dairy cows with sub-clinical mastitis.

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Expression patterns of major genes in fatty acid synthesis, inflammation, oxidative stress pathways from colostrum to milk in Damascus goats

Cited by 7 publications

References 57 publications

Associations between Somatic Cell Count and Milk Fatty Acid and Amino Acid Profile in Alpine and Saanen Goat Breeds

Associations between Somatic Cell Count and Milk Fatty Acid and Amino Acid Profile in Alpine and Saanen Goat Breeds

NRF2 is a potential biomarker for the evaluation of weaning-related oxidative stress in goat kids

Relationship of Oxidative Stress and Some Blood Parameters with Quarters Affected by Mastitis in Dairy Cows

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