Expression patterns of diverse genes in response to gamma irradiation inNicotiana tabacum

Cho, Hye Sun; Lee, Heang Soon; Pai, Hyun Sook

doi:10.1007/bf03030499

Cited by 28 publications

(19 citation statements)

References 22 publications

(18 reference statements)

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“…The supernatant (1 ml) obtained was mixed with an equal INFLUENCE OF IONIZING IRRADIATION ON THE ANTIOXIDANT ENZYMES OF VICIA FABA L hydrogen peroxide to water and oxygen (Cakmak and Horst, 1991). Moreover Cho et al (2000) found that the expression patterns of GST, SOD, POD and CAT genes exhibited increased transcripts with γ-irradiation of Nicotiana tabacum. Slooten et al (1995) reported that the stimulation in SOD activity in response to stresses is possibly attributed to the de-novo synthesis of the enzymatic protein.…”

Section: Determination Of Lipid Peroxidationmentioning

confidence: 99%

Influence of ionizing irradiation on the antioxidant enzymes of <i>Vicia faba</i> L.

Aly¹,

El-Beltagi²

2010

Grasas y Aceites

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288SOD, but for GST enzyme at a dose level 10.0 kGy. For HeNe laser, with or without different doses (2.5, 5.0, 10.0 and 20.0 kGy) of γ-irradiation, enzyme induction was significantly stimulated and positively correlated with the dose levels of γ-irradiation in combination with the He-Ne treatment. There was a significant increase in the concentration of MDA and this increase was more pronounced at dose level 20.0 kGy (38.2 µmol/g d.w) compared to the control (3.9 µmol/g d.w). Laser treatment by He-Ne laser or diode laser only caused a slight increase (P<0.05) in MDA content (4.4 and 5.08 µmol/g d.w respectively) compared to the control (3.9 µmol/g d.w). H 2 O 2 content significantly increased in all treatments and this increase reached its maximum at dose level 20.0 kGy (36.3 µmol/g d.w) compared to the control (2.3 µmol/g d.w.). On the other hand, He-Ne or diode laser treatments combined with γ-irradiation significantly decreased in comparison with γ-irradiation treatments alone. In the case of glutathione content, there were significant increases by γ-irradiation at dose levels 2.5, 5.0, 10.0 and 20.0 kGy. Furthermore, with a combination of γ-irradiation and He-Ne or diode laser, a marked increase in glutathione content was found and was more pronounced than that of gamma irradiation alone. KEY-WORDS: Antioxidants enzyme -Gamma irradiationLaser irirradiation -Vicia faba L. INTRODUCTIONUnder natural conditions of growth and development, plants are inevitably exposed to different types of stress, which may cause an increased production of active oxygen species (ROS) (Smirnoff, 1993 ), which cause tissue injury. Plants have evolved various protective mechanisms to eliminate or reduce ROS. In plant cells, one such protective mechanism is an antioxidant system, composed of both non-enzymatic and enzymatic antioxidants (Foyer et al., 1994b). The capacity of the antioxidant defense system is often increased under stress conditions (Gressel and Galun, 1994), but in most situations the response is moderate (Foyer et al., 1994a). ROS are highly reactive in the absence of any protective mechanism, they can seriously disrupt normal metabolism through oxidative damage to membrane lipids, proteins, pigments and nucleic acids. These ROS are detoxified by the sequential and RESUMEN Influencia de la radiación ionizante en enzimas antioxidantes de Vicia faba L.Semillas de Vicia faba L. Giza 834 fueron expuestas a γ-irradiación a dosis de 2.5, 5.0, 10.0 y 20.0 kGy y después expuesta a una radiación laser de He-Ne (632.8 nm) o de diodo (650.0 nm) durante 5 minutos. Las actividades de las enzimas POD, APOX, CAT, SOD y GST fueron favorecidas significativamente, y éstas alcanzaron un máximo a una dosis de 5.0 kGy para las enzimas POD, APOX, CAT y SOD, y a 10.0 kGy para la enzima GST. Más aún, a diferentes dosis de γ-irradiación (2.5, 5.0, 10.0 and 20.0 kGy) con y sin laser de He-Ne, la inducción enzimática fue significativamente favorecida correlacionando positivamente con las dosis de γ-irradiación en combinación con tratamientos ...

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Section: Determination Of Lipid Peroxidationmentioning

confidence: 99%

Influence of ionizing irradiation on the antioxidant enzymes of <i>Vicia faba</i> L.

Aly¹,

El-Beltagi²

2010

Grasas y Aceites

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“…Aly and El-Beltagi [38] showed that increase in GST, CAT, SOD, and POD activities in Vicia faba L. seeds could be attributed to ionizing irradiation stress. Cho et al [39] investigated the expression patterns of diverse genes at various time points after gamma irradiation of young tobacco plants and found three different gene expression pattern (increased, decreased, and no response) of antioxidative enzymes (CAT, SOD, and GST). However, Al-Rumaih and Al-Rumaih [40] reported that gamma irradiation affected antioxidant enzyme activities in the three investigated species of wheat.…”

Section: Ionize Radiation Reactive Oxygen Species (Ros) and Defense mentioning

confidence: 99%

The Use of Gamma Irradiation in Plant Mutation Breeding

Beyaz¹,

Yıldız²

2017

Plant Engineering

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In plant breeding programs, one of the oldest methods is mutation breeding. Currently, mutation breeding has became popular among the breeders and scientists again with its use in plant biotechnology and due to some restrictions on the other techniques such as hybridization, cross breeding, and transgenic plants. Physical mutagens (X-rays, UV light, neutrons-alpha-beta particles, fast and thermal neutrons, especially gamma rays) are used more widely than chemical (ethyl methanesulfonate [EMS]) ones to artificially induce mutations (mutagenesis). However, among the physical mutagens, gamma-rays are widely used. During the irradiation of the seeds with ionizing radiation to generate mutants with desirable traits, reactive oxygen species (ROS) or free radicals can generate in cells. Although, these radicals/species generally can be very dangerous for the cell compartments, they can take an important role as a signal molecule activation of genes of antioxidant enzymes and proline, which are defense systems against these radicals in plant cells. In this chapter, usability of gamma-irradiation to provide the permanent gene expression of antioxidant enzymes and proline through the production of reactive oxygen species (ROS) is discussed.

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“…The stress reaction of plants often results in an alteration of gene products (Humera, 2006), which plays a key role in the molecular mechanisms of stress tolerance (Rao, 2006). Cho et al (2000) demonstrated that various genes displayed differential patterns of gene expression in response to irradiation in tobacco plants, thereby suggesting that a complex signaling mechanism is involved in the irradiation-induced defense by plants. As a result of altered gene expression under radiation stress, the qualitative and quantitative changes in protein content were obvious (Corthals et al, 2000).…”

Section: Total Soluble Protein Contentmentioning

confidence: 99%

“…Meanwhile, Cho et al (2000) discovered that a group of genes, which includes glutathione-S-transferase, peroxidase, superoxide dismutase, and catalase, showed stimulation of transcript levels upon γ irradiation of young tobacco plants. This suggests that there was an increase in expression of peroxidase and its activity in young tobacco plants which were subjected to radiation stress.…”

Section: Specific Activity Of Peroxidasementioning

confidence: 99%

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

Ling

Ung

Hussein

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses (10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam (10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics.

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Expression patterns of diverse genes in response to gamma irradiation inNicotiana tabacum

Cited by 28 publications

References 22 publications

Influence of ionizing irradiation on the antioxidant enzymes of <i>Vicia faba</i> L.

Influence of ionizing irradiation on the antioxidant enzymes of <i>Vicia faba</i> L.

The Use of Gamma Irradiation in Plant Mutation Breeding

Morphological and biochemical responses of Oryza sativa L. (cultivar MR219) to ion beam irradiation

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