Expression Pattern of Genes of RLR-Mediated Antiviral Pathway in Different-Breed Chicken Response to Marek’s Disease Virus Infection

Feng, Zeqing; Lian, Ting; Huang, Yong; Zhu, Qing; Liu, Yiping

doi:10.1155/2013/419256

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…The role of IFNs in the control of MDV replication has been confirmed in an in vitro model showing that IFNs reduces plaque formation and expression of PP38 and gB in the infected cells [77]. The protective role of IFNs is also indicated by the results demonstrating the differential expression patters of IRF-3 and IFN-β genes in resistant and susceptible chicken lines [78]. Similarly, oral administration of IFN-α are shown to reduce MDV viral replication in vivo [79].…”

Section: The Immune Response To MDVmentioning

confidence: 97%

Marek’s disease in chickens: a review with focus on immunology

Boodhoo

Gurung

Sharif

et al. 2016

Vet Res

139

130

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Marek’s disease (MD), caused by Marek’s disease virus (MDV), is a commercially important neoplastic disease of poultry which is only controlled by mass vaccination. Importantly, vaccines that can provide sterile immunity and inhibit virus transmission are lacking; such that vaccines are only capable of preventing neuropathy, oncogenic disease and immunosuppression, but are unable to prevent MDV transmission or infection, leading to emergence of increasingly virulent pathotypes. Hence, to address these issues, developing more efficacious vaccines that induce sterile immunity have become one of the important research goals for avian immunologists today. MDV shares very close genomic functional and structural characteristics to most mammalian herpes viruses such as herpes simplex virus (HSV). MD also provides an excellent T cell lymphoma model for gaining insights into other herpesvirus-induced oncogenesis in mammals and birds. For these reasons, we need to develop an in-depth knowledge and understanding of the host-viral interaction and host immunity against MD. Similarly, the underlying genetic variation within different chicken lines has a major impact on the outcome of infection. In this review article, we aim to investigate the pathogenesis of MDV infection, host immunity to MD and discuss areas of research that need to be further explored.

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Section: The Immune Response To MDVmentioning

confidence: 97%

Marek’s disease in chickens: a review with focus on immunology

Boodhoo

Gurung

Sharif

et al. 2016

Vet Res

139

130

View full text Add to dashboard Cite

show abstract

“…An aerosol challenge with cell-free MDV significantly increases the expression of IL-1β, CXCLi2, iNOS, TLR3 and TLR7 in the lungs of 5-day-old chickens (Abdul-Careem et al, 2007b). Feng et al (2013) showed differences in RIG-I-like receptors and downstream antiviral response pathways to be influenced by the genetic background of newly hatched chickens infected with MDV (Feng et al, 2013). The innate responses of chicken embryos to influenza virus infection using ex vivo and in vivo experiments resemble responses to infections with other respiratory pathogens such as Newcastle disease virus.…”

Section: Viral Infectionsmentioning

confidence: 98%

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

et al. 2019

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Newly hatched chickens are confronted by a wide array of pathogenic microbes because their adaptive immune defences have limited capabilities to control these pathogens. In such circumstances, and within this age group, innate responses provide a degree of protection. Moreover, as the adaptive immune system is relatively naïve to foreign antigens, synergy with innate defences is critical. This review presents knowledge on the ontogeny of innate immunity in chickens pre-hatch and early post-hatch and provides insights into possible interventions to modulate innate responses early in the life of the bird. As in other vertebrate species, the chicken innate immune system which include cellular mediators, cytokine and chemokine repertoires and molecules involved in antigen detection, develop early in life. Comparison of innate immune systems in newly hatched chickens and mature birds has revealed differences in magnitude and quality, but responses in younger chickens can be boosted using innate immune system modulators. Functional expression of pattern recognition receptors and several defence molecules by innate immune system cells of embryos and newly hatched chicks suggests that innate responses can be modulated at this stage of development to combat pathogens. Improved understanding of innate immune system ontogeny and functionality in chickens is critical for the implementation of sound and safe interventions to provide long-term protection against pathogens. Next-generation tools for studying genetic and epigenetic regulation of genes, functional metagenomics and gene knockouts can be used in the future to explore and dissect the contributions of signalling pathways of innate immunity and to devise more efficacious disease control strategies.

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“…Surprisingly, in chickens, only IRF3 was found [ 36 – 38 ]. Moreover, chicken IRF3, which is closely related to mammalian IRF7 in structure, may induce IFNβ expression after virus or dsRNA stimulation [ 37 , 39 ]. This information suggestes that the ch TBK1 gene may induce chIRF3 and IFNβ expression.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization, expression of chicken TBK1 gene and its effect on IRF3 signaling pathway

et al. 2017

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TRAF family member-associated NF-κB activator (TANK)-binding kinase1 (TBK1) is a serine-threonine kinase at the crossroads of multiple interferon (IFN)-inducing signaling pathways in innate immunity. The importance of TBK1 in antiviral immunity is well established in mammal models, but in chicken, the molecular characterization and potential function of TBK1 remain unclear. In the present study, the open-reading frame (ORF) of chicken TBK1 (chTBK1) was cloned and characterized. The sequencing results revealed that the chTBK1 ORF consists of 2190 base pairs (bp) encoding a deduced protein of 729 amino acid residues. Multiple sequence alignment analysis demonstrated chTBK1 similarity to other birds and mammals, which indicates that it is evolutionarily conserved. Quantitative real-time PCR (qRT-PCR) results showed that chTBK1 was ubiquitously expressed in chicken tissues and expression was especially high in immune tissues. In addition, the expression of chTBK1 was significantly up-regulated by infection with avian leukosis virus subgroup J (ALV-J) both in vivo and in chicken embryo fibroblasts (CEFs) challenged with ALV-J or stimulated with poly I:C in vitro. Consistent with the activation of chTBK1, the interferon regulatory factor 3 (IRF3) and IFNβ gene in CEFs were also up-regulated after challenge with ALV-J or polyI:C. In contrast, the expression of IRF3 and IFNβ in CEFs was significantly reduced by siRNA targeting the chTBK1 gene compared with a negative control (NC) during ALV-J infection or polyI:C transfection. In conclusion, our results demonstrated that chTBK1 may be an important immunoregulator for IRF3 and IFNβ induction in response to viral stimulation in chicken.

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Expression Pattern of Genes of RLR-Mediated Antiviral Pathway in Different-Breed Chicken Response to Marek’s Disease Virus Infection

Cited by 9 publications

References 46 publications

Marek’s disease in chickens: a review with focus on immunology

Marek’s disease in chickens: a review with focus on immunology

Development of innate immunity in chicken embryos and newly hatched chicks: a disease control perspective

Molecular characterization, expression of chicken TBK1 gene and its effect on IRF3 signaling pathway

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