Expression ofras, c-myc, and p53 proteins in cervical intraepithelial neoplasia

Slagle, Betty L.; Kaufman, Raymond H.; Reeves, William C.; Icenogle, Joseph P.

doi:10.1002/(sici)1097-0142(19981001)83:7<1401::aid-cncr18>3.0.co;2-8

Cited by 19 publications

(5 citation statements)

References 28 publications

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“…Some reported that MYC expression level was elevated from CIN I to CIN III, 13 whereas others reported that MYC expressions did not differ significantly among CIN biopsies. 16 Our results supported that MYC protein levels were increasing in the early stage (CIN and cancer stage I), but they did not continue increasing in more severe stages. The ROC curve analysis indicated that MYC expression variation could not distinguish between CIN and cervical cancer statistically (P = 0.393; Table 4).…”

Section: Discussionsupporting

confidence: 57%

Atl

Zhu

Jiang

et al. 2014

Int J Gynecol Cancer

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Section: Discussionsupporting

confidence: 57%

Atl

Zhu

Jiang

et al. 2014

Int J Gynecol Cancer

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“…The genetic changes taking place during the procedure of carcinogenesis of the female lower genital tract and their clinical impact have not been fully elucidated. Many studies have focused on the expression of several different proteins in normal, precancerous, and cancerous lesions of the cervix with refuting results (18–20) . Similar studies have not been undertaken for the vagina and thus, an interesting finding in the present study was the negativity for p53 overexpression after treatment, in three patients who had p53‐positive nuclei prior to the beginning of therapy.…”

Section: Discussionmentioning

confidence: 55%

Can local application of imiquimod cream be an alternative mode of therapy for patients with high-grade intraepithelial lesions of the vagina?

Haidopoulos¹,

Diakomanolis²,

Rodolakis³

et al. 2005

Int J Gynecol Cancer

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The aim of the present study was to assess the local application of imiquimod cream 5% as an alternative mode of therapy for high-grade vaginal intraepithelial neoplasia (VAIN 2/3). Positive human papillomavirus (HPV) patients with multifocal high-grade VAIN (2/3) not involving the vaginal vault in hysterectomized patients took part in this study. The treatment consisted of vaginal application of the cream under colposcopic guidance. Following management, biopsies were obtained from the previously recorded lesions. p53 expression was recorded prior and after therapy. Seven patients with VAIN 2/3 took part in this study. Six patients (86%) were positive for high-risk HPV type while three (43%) women who were positive for p53 nuclei prior to therapy were found to be negative following treatment. After treatment, 86% of the patients were found to have either HPV infection or low-grade VAIN. During follow-up, two patients (28.5%) were managed by vaginectomy, one for persistent and one for recurrent high-grade VAIN. Currently, from the five patients that are followed, three have simple HPV infection and two, VAIN 1. Imiquimod cream 5% might represent an alternative although not permanent method of management in young, HPV-positive women with multifocal high-grade lesions of the vagina (VAIN 2/3).

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“…Several immunohistochemical studies on the expression of p53 in cervical intraepithelial lesions have been recently conducted and commonly report an increased frequency of p53 overexpression in HSIL as compared to LSIL . On the other hand there are also reports, albeit limited in number, suggesting that the levels of p53 overexpression may be higher in low grade lesions as compared to higher grade dysplasia or that there is no correlation whatsoever between p53 expression and intraepithelial disease grade . Significant discrepancies also exist with respect to the reported frequencies of p53 overexpression among LSIL or HSIL cases, respectively, as different studies report widely varied rates.…”

Section: Discussionmentioning

confidence: 98%

“…Several previous studies have investigated p53 protein expression in cervical intraepithelial lesions and in invasive carcinomas, but some of these results are contradictory . Furthermore, the published data on the expression of PTEN and Fas proteins in cervical intraepithelial neoplasia are rather limited, while, to the best of our knowledge, there are no previous immunocytochemical studies on the expression of these proteins in liquid‐based cervical smears.…”

mentioning

confidence: 95%

“Immunocytochemical expression of P53, PTEN, FAS (CD95), P16INK4A and HPV L1 major capsid proteins in ThinPrep cervical samples with squamous intraepithelial lesions”

Grapsa

Frangou-Plemenou

Kondi-Pafiti

et al. 2013

Diagnostic Cytopathology

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The aim of this study was to further investigate the immunocytochemical expression of p53, PTEN, Fas, p16, and HPV L1 capsid proteins in cervical smears with low and high grade squamous intraepithelial lesions (LSIL and HSIL, respectively). A total of 92 ThinPrep cervical samples, comprising 11 cases of HSIL, 61 cases of LSIL, and 20 negative cases were studied by immunocytochemical methods. The results obtained in LSIL cases were correlated with the available follow up data. Abnormal p53, PTEN, or Fas expression was found in a subset of HSIL cases, while positive expression for p16 was significantly associated with the diagnosis of HSIL (P < 0.0001, P = 0.001, P < 0.0001, and P < 0.0001, respectively). Among cases positive for p16 expression, the staining pattern was weak in 88.9% of LSIL cases and strong in 80% of HSIL cases (P < 0.0001). The p16 negative/L1 positive and p16 positive/L1 negative staining patterns were significantly associated with the presence of LSIL and HSIL, respectively (P < 0.0001). None of these markers had a significant prognostic value in LSIL cases (P > 0.05). Our results suggest that loss of PTEN or Fas expression and p53 overexpression may be involved in the process of neoplastic transformation of the cervical epithelium. Furthermore, negative or weak immunocytochemical staining for p16 in a Pap smear may strongly argue against the presence of a high grade lesion, while the combined p16/L1 staining pattern may be useful as a diagnostic adjunct for differentiating between LSIL and HSIL.

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Expression ofras, c-myc, and p53 proteins in cervical intraepithelial neoplasia

Cited by 19 publications

References 28 publications

Atl

Atl

Can local application of imiquimod cream be an alternative mode of therapy for patients with high-grade intraepithelial lesions of the vagina?

“Immunocytochemical expression of P53, PTEN, FAS (CD95), P16INK4A and HPV L1 major capsid proteins in ThinPrep cervical samples with squamous intraepithelial lesions”

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