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Zhu, Da; Jiang, Xiaohui; Jiang, Yunhui; Ding, Wencheng; Zhang, Chang-Lin; Shen, Hui; Wang, Xiaoli; Ma, Ding; Hu, Zheng; Wang, Hui

doi:10.1097/igc.0000000000000122

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…For example, upregulation of p120ctn was seen in 37 % of gastric dysplasia cases and 66 % of gastric carcinoma cases in one study, sometimes associated with reduced membranous expression [ 31 ]. Also, c-myc copy number gain has been found to play a key role in the process of disease progression in cervical dysplasia [ 32 , 33 ]. CDX2 staining has shown varying results in gastric neoplasia.…”

Section: Discussionmentioning

confidence: 99%

Increasing diagnostic accuracy to grade dysplasia in Barrett’s esophagus using an immunohistochemical panel for CDX2, p120ctn, c-Myc and Jagged1

et al. 2016

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BackgroundPatients with non-dysplastic Barrett’s esophagus (ND-BE) and low-grade dysplasia (LGD) are typically monitored by periodic endoscopic surveillance, while those with high-grade dysplasia (HGD) and esophageal adenocarcinoma (EAC) are usually treated by more aggressive interventions like endoscopic mucosal resection, ablation or surgery. Therefore, the accurate grading of dysplasia in Barrett’s esophagus (BE) is essential for proper patient care. However, there is significant interobserver and intraobserver variability in the histologic grading of BE dysplasia. The objective of this study was to create an immunohistochemical (IHC) panel that facilitates the grading of BE dysplasia and can be used as an adjunct to histology in challenging cases.Methods100 BE biopsies were re-graded for dysplasia independently by 3 subspecialized gastrointestinal pathologists. IHC staining for CDX2, p120ctn, c-Myc and Jagged1 proteins was then performed and assessed by two separate methods of semi-quantitative scoring. Scores were integrated using a principal component analysis (PCA) and receiver operating characteristic (ROC) curve.ResultsPrincipal component analysis demonstrated the ability of this panel of proteins to segregate ND-BE/LGD and HGD/EAC, as the expression of the four proteins is significantly altered between the two subsets. Analysis of the receiver operating characteristic curve showed that this panel has the potential to aid in the grading of dysplasia in these two subcategories with both high sensitivity and specificity. While not able to discriminate between ND-BE and LGD, this panel of four proteins may be used as an adjunct to help discriminate subsets of ND-BE/LGD from HGD/EAC.ConclusionsWe propose that the maximum utility of this IHC panel of CDX2, p120ctn, c-Myc, and Jagged1 proteins would be to distinguish between LGD and HGD in histologically challenging cases, given the aggressive interventions still used for HGD in many institutions, and hence may aid in the optimal patient management. The results of this initial study are promising, though further validation is needed before this panel can be used clinically, including future randomized prospective studies with larger patient cohorts from diverse locations.Electronic supplementary materialThe online version of this article (doi:10.1186/s13000-016-0473-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Increasing diagnostic accuracy to grade dysplasia in Barrett’s esophagus using an immunohistochemical panel for CDX2, p120ctn, c-Myc and Jagged1

et al. 2016

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“…It thus makes it possible to identify a panel of useful tumor-associated antigens (TAAs) for the development of antibody-based test for early diagnosis of cervical cancer. Increased expression of baculoviral IAP repeat-containing protein 5 isoform 2 (BIRC5) and myc proto-oncogene protein (MYC) have been reported in cervical cancer [1,10,20] ; to our knowledge, it has not been documented whether antibodies against BIRC5 and MYC proteins are also increased in this malignant disease. Accordingly, the present work was undertaken to detect circulating IgG antibodies for BIRC5 and MYC among patients with cervical cancer and control subjects in a Chinese population.…”

Section: Introductionmentioning

confidence: 94%

Study of circulating IgG antibodies to peptide antigens derived from BIRC5 and MYC in cervical cancer

Jin

Liu

et al. 2015

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“…In recent years, the excessive surgical treatment of CIN has caused a series of adverse effects, including infertility, miscarriage, premature delivery, cervical insufficiency and cesarean section in subsequent pregnancies. This has caused great physical and mental stress, especially in young patients with childbearing desires, and has thus become a focus of investigation, 4 , 5 as understanding the factors that predict progression of CIN is of critical value.…”

Section: Introductionmentioning

confidence: 99%

Association between folate status and cervical intraepithelial neoplasia

et al. 2016

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Background/Objectives:To investigate the effect of folate status on cervical intraepithelial neoplasia (CIN) progression and its relationship with high-risk human papillomavirus (hrHPV).Subjects/Methods:We evaluated 20 000 sexually active women aged <65 years in Yangqu County by using a questionnaire; the subjects were also screened using the ThinPrep cytologic test (TCT). Patients with abnormal TCT results (other than glandular cell abnormalities) who were willing to provide informed consent were further diagnosed using colposcopy and histopathological examination. We investigated 247 cases of low-grade cervical squamous intraepithelial lesions (LSIL), 125 cases of high-grade cervical squamous intraepithelial lesions (HSIL) and 877 controls. A 24-item food frequency questionnaire was filled out by the investigator to estimate the consumption of dietary folate. Positivity for hrHPV from residual exfoliated cervical cells was tested; serum folate was also measured.Results:The hrHPV infection rate in HSIL patients (77.6%) was higher than that in LSIL (33.2%) and control (32.0%) patients. Dietary folate intakes in controls, LSIL and HSIL were 306.9±176.6, 321.8±168.0 and 314.7±193.8 μg/kcal, respectively. The levels of serum folate in controls, LSIL and HSIL were 18.2±7.9, 15.9±7.1 and 14.3±7.5 nmol/l, respectively. Increased CIN correlated with higher rates of hrHPV infection and lower levels of serum folate.Conclusions:Low levels of serum folate may increase the risk of CIN progression. Furthermore, potential synergy may exist between low serum folate levels and hrHPV infection to promote CIN development.

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Abstract: Amplification and overexpression of TP63 and MYC, and HPV integration rate, are associated with the transition of CIN to cervical cancer. Future studies on these biomarkers will help to assess the risk of CIN progression.

Cited by 15 publications

References 18 publications

Increasing diagnostic accuracy to grade dysplasia in Barrett’s esophagus using an immunohistochemical panel for CDX2, p120ctn, c-Myc and Jagged1

Increasing diagnostic accuracy to grade dysplasia in Barrett’s esophagus using an immunohistochemical panel for CDX2, p120ctn, c-Myc and Jagged1

Study of circulating IgG antibodies to peptide antigens derived from BIRC5 and MYC in cervical cancer

Association between folate status and cervical intraepithelial neoplasia

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