Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

Abstract

Author contributions: Ye Q designed and performed the research, including performance of colony formation assay, cell migration and invasion assay and writing the paper; Feng B participated in designing the research and carried out immunohistochemistry; Peng YF performed isolation of RNA and protein, and carried out western blotting and CCK8 assay; Chen XH cultured cells, and carried out selection of stable transfectants; Cai Q carried out real-time quantitative RT-PCR; Yu BQ carried out plasmid construction; Li LH and Qiu MY collected tissue samples and clinicopathological data used in the study; Liu BY contributed vital reagents and analytical tools; Zheng MH took responsibility for financial support, and obtaining permission from all coauthors for the submission of any version of the paper and for any changes in authorship. RESULTS:The expression of γ-synuclein mRNA and protein was much higher in CRC tissue samples than in NNAT samples (P = 0.02, P = 0.036). There was a significant correlation between the γ-synuclein protein expression and clinical stage and lymph node involvement of CRC (P = 0.02, P = 0.033). In functional analysis we found that down-regulation of γ-synuclein expression in HCT116 cells could inhibit the growth, colony formation rate, and migration and invasion ability of HCT116 cells. CONCLUSION:Increased expression of γ-synuclein in CRC tissues and the biological effects of reduced γ-synuclein expression on HCT116 cells suggest that γ-synuclein may play a positive role in the progression of CRC.

γ-Synuclein confers both pro-invasive and doxorubicin-mediated pro-apoptotic properties to the colon adenocarcinoma LS 174T cell line

“…γ-synuclein enhances the motility of LS 174T cells in the scratch wound assay. Consistent with previous findings, γ-synuclein plays a role in enhancing the migration of colorectal [11] and prostate [10] cancer cells to the lower Boyden chamber.…”

“…Although similar studies have been conducted previously, a concrete conclusion on the effects of γ-synuclein expression in colorectal cell lines still could not be drawn, due to the different cell lines used [11,24,25]. Besides, although considered raising from similar origin, different colorectal cancer cell lines have shown widely different patterns of growth and metastatic spread in the in vivo orthotopic mice model [26].…”

“…Resistance to de-adhesion-induced apoptosis (anoikis), which is regarded as a crucial step during tumorigenesis, was also decreased in LS 174T cells overexpressing γ-synuclein. Being proposed as a pro-invasive protein, majority of authors reported that overexpression of γ-synuclein was able to enhance the growth of cancer cells while on the other hand, its down-regulation could suppress the growth of the cells under anchorage-dependent and -independent manners in vitro [11,[34][35][36]. However, Zhou et al [12] reported that overexpression of γ-synuclein in oesophageal cancer ESCC 9706 cells could inhibit its growth rate and transformation ability.…”

“…On the other hand, knockdown of γ-synuclein sensitised human breast cancer cells to endoplasmic reticulum stress-induced apoptosis [9], while silencing of γ-synuclein in LNCaP prostate cancer cells contributed to the inhibition of cellular proliferation through induction of cell cycle arrest at the G1 phase [10]. Ye et al [11] found that there was increased γ-synuclein expression in colorectal cancer tissues and knockdown assay on HCT116 colorectal cancer cell line inhibited its growth and colony formation rate, migration and invasion ability. However, Zhou et al [12] had an opposite finding in oesophagus cancer, in which low expression levels of γ-synuclein in human oesophageal squamous cell carcinoma (ESCC) tissues was found, while overexpression of γ-synuclein in ESCC 9706 cell line could inhibit its growth rate and transformation ability.…”

Novel Biomarkers in Pancreatic Cancer

“…Increased expression of aSyn has been also identified in melanoma , while its isoform gamma-synuclein (cSyn) has been shown to be elevated in tumors of breast, uterine, colorectal and pancreas (Ye et al, 2009;Hibi et al, 2009;Ahmad et al, 2007;Li et al, 2004;Gupta et al, 2003;Jia et al, 1999;Morgan et al, 2009). Moreover, due to the structural omology between cSyn and aSyn, these factors potentiate invasion in these tumors.…”