Expression of β-defensins in bovine oviduct and uterus during follicular and luteal phases

Zeng, Yicheng; Adegoke, Elikanah Olusayo; Wang, Xue; Xu, Lin; Wang, Hao; Wang, Chen; Zhang, Guixue

doi:10.1007/s00580-017-2442-2

Cited by 8 publications

(14 citation statements)

References 35 publications

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“…This finding is also following several reports which explained that increasing levels of E2 at the estrus phase could affect the histomorphological changes and functions of the uterine glands (Gonella-Diaza et al, 2017;Núñez-Olivera et al, 2020). During the follicular phase (proestrus-estrus), it was reported an increase in the number of estradiol receptors α (ERα) in three parts of the uterus (Zeng et al, 2017;Perry et al, 2021). The increase in plasma E2 concentrations in the circulation and the increasing existence of Erα will cause the lengthening of uterine glands, followed by the production of estrus mucus by ciliated epithelial cells of the endometrium and secretory cells (Bunma et al, 2021).…”

Section: Discussionsupporting

confidence: 88%

Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration

Putro

Winarto

Amrozi

et al. 2023

Trop. Anim. Sci. J.

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Injection of female mammalian animals with gonadotropin prior to mating using superovulation dose increases estradiol 17beta (E2) synthesis and secretion and improves the growth and development of the uterus to support prenatal growth with the side effect of increasing litter size. This experiment was designed to study the effects of low doses of PMSG injection in heifers on the growth of granulose cells (GC) of pre-ovulatory follicles, E2 synthesis, and uterine glands growth and development. Nine Ongole-grade heifers were divided into three groups of doses of PMSG injection, i.e., 0 IU/kg BW, 0.5 IU/kg BW, and 1.0 IU/kg BW. The injection of pregnant mare serum gonadotropin (PMSG) was conducted at the early stage of the second follicle wave. Blood samples were collected at estrus (day 0) to measure plasma E2 concentrations. The ovary and uterus were collected for histological observation after slaughtering the animal. The results showed that non-superovulation doses of PMSG injections at the second follicle wave significantly increased the growth of GC that eventually produced a significantly higher plasma E2 concentration by 39.47% and 113.57%, in heifers receiving 0.5 IU/kg BW and 1.0 IU/kg BW (p<0.05), respectively. The increase in E2 synthesis and secretion significantly stimulated the growth and development of uterine glands (p<0.05), which was reflected in the improved measures of uterine glands. In conclusion, injections of heifers with low doses of PMSG at the second follicle wave could increase the growth of GC to produce a higher secretion of E2 that eventually improves the growth and development of uterine glands in the endometrium without the risk of multiple calving.

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Section: Discussionsupporting

confidence: 88%

Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration

Putro

Winarto

Amrozi

et al. 2023

Trop. Anim. Sci. J.

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“…Numerous studies have demonstrated the presence of diverse BDs within the endometrial and oviductal epithelium in humans [ 110 , 112 ], mice [ 113 ], and bovines [ 114 , 115 ]. For example, BDs have been shown to be expressed at the transcriptional levels in the human UECs (including human BD ( HBD ) -1 , HBD-2 , HBD-3 , and HBD-4 ) and at the protein levels in the murine UECs, including mouse BD (mBD)-1, mBD-2, mBD-3, and mBD-4 [ 110 , 112 , 113 ].…”

Section: Antibacterial Activity Of the Reproductive Epithelial Cellsmentioning

confidence: 99%

“…Alan and Liman [ 113 ] reported that during diestrus, mBD-1, mBD-2, mBD-3, and mBD-4 exhibit cytoplasmic and nuclear expressions within diverse constituents of the murine UECs, including luminal and glandular epithelial cells. Furthermore, it has been documented that HBDs and bovine BDs (BNBDs) exhibit an organ-specific pattern of expression [ 65 , 114 ]. As evidenced by recent research, BNBD-1 , BNBD-4 , BNBD-5 , and BNBD-10 exhibit elevated expression levels within the bovine uterus when compared to the oviduct [ 114 ].…”

Section: Antibacterial Activity Of the Reproductive Epithelial Cellsmentioning

confidence: 99%

“…Furthermore, it has been documented that HBDs and bovine BDs (BNBDs) exhibit an organ-specific pattern of expression [ 65 , 114 ]. As evidenced by recent research, BNBD-1 , BNBD-4 , BNBD-5 , and BNBD-10 exhibit elevated expression levels within the bovine uterus when compared to the oviduct [ 114 ]. Similarly, Quayle et al.…”

Section: Antibacterial Activity Of the Reproductive Epithelial Cellsmentioning

confidence: 99%

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Ovarian sex steroid and epithelial control of immune responses in the uterus and oviduct: human and animal models

Kowsar,

Sadeghi,

Hashemzadeh

et al. 2023

Biology of Reproduction

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The female reproductive tract (FRT), including the uterus and oviduct (Fallopian tube), is responsible for maintaining an optimal microenvironment for reproductive processes, such as gamete activation and transportation, sperm capacitation, fertilization, and early embryonic and fetal development. The mucosal surface of the FRT may be exposed to pathogens and sexually transmitted microorganisms due to the opening of the cervix during mating. Pathogens and endotoxins may also reach the oviduct through the peritoneal fluid. To maintain an optimum reproductive environment while recognizing and killing pathogenic bacterial and viral agents, the oviduct and uterus should be equipped with an efficient and rigorously controlled immune system. Ovarian sex steroids can affect epithelial cells and underlying stromal cells, which have been shown to mediate innate and adaptive immune responses. This, in turn, protects against potential infections while maintaining an optimal milieu for reproductive events, highlighting the homeostatic involvement of ovarian sex steroids and reproductive epithelial cells. This article will discuss how ovarian sex steroids affect the immune reactions elicited by the epithelial cells of the non-pregnant uterus and oviduct in the bovine, murine, and human species. Finally, we propose that there are regional and species-specific differences in the immune responses in FRT.

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“…The TLR2/TLR6 complex is activated by LTA and diacylated lipoproteins, but not by triacylated lipoproteins ( Figure 4B ). Besides cytokines, the principal effector of mucosal defense in pathogens, antimicrobial peptides (AMPs) were found to be involved in the physiological role of the body and had been recognized as part of the innate immune system ( 143 , 144 ). β-Defensin (DEFB) is a family of AMPs that can permeate bacterial membranes, including DEFB1, DEFB4A, and DEFB5, linguistic AMP, and tracheal AMP ( 145 ), which is central to the AMP family.…”

Section: Relationships Between Mirnas and Endometrium Toll-like Recepmentioning

confidence: 99%

MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis

Oladejo

et al. 2020

Front. Vet. Sci.

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The bovine endometrium is a natural pathogen invasion barrier of the uterine tissues' endometrial epithelial cells that can resist foreign pathogen invasion by controlling the inflammatory immune response. Some pathogens suppress the innate immune system of the endometrium, leading to prolonged systemic inflammatory response through the blood circulation or cellular degradation resulting in bovine endometritis by bacterial endotoxins. The microRNA (miRNA) typically involves gene expression in multicellular organisms in post-transcription regulation by affecting both the stability and the translation of messenger RNA. Accumulated evidence suggests that miRNAs are important regulators of genes in several cellular processes. They are a class of endogenous non-coding RNAs, which play pivotal roles in the inflammatory response of reproductive diseases. Studies confirmed that miRNAs play a key regulatory role in various inflammatory diseases by mediating the molecular mechanism of inflammatory cytokines via signal pathways. It implicates some miRNAs in the occurrence of bovine endometritis, resorting to regulating the activities of some inflammatory cytokines, chemokine, differentially expressed genes, and protein through modulating of specific cellular signal pathways functions. This review dwells on improving the knowledge of the role of miRNAs involvement in inflammatory response as to early diagnosis, control, and prevention of bovine endometritis and consequently enlighten on the molecular improvement of the genes coded by various differentially expressed miRNA through the need to adopt recent genetic technologies and the development of new pharmaceutical preparations.

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Expression of β-defensins in bovine oviduct and uterus during follicular and luteal phases

Cited by 8 publications

References 35 publications

Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration

Quantitative Histomorphometry of Pre-ovulatory Follicles and Uterine Glands of Ongole-grade Heifer in Response to the Low Doses of PMSG Administration

Ovarian sex steroid and epithelial control of immune responses in the uterus and oviduct: human and animal models

MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis

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