Expression of Zm13, a pollen specific maize protein, in <i>Escherichia coli</i> reveals IgE‐binding capacity and allergenic potential

Heiss, Susanne; Flicker, Sabine; Hamilton, Douglas A.; Mascarenhas, Joseph P.; Valenta, Rudolf

doi:10.1016/0014-5793(96)00108-1

Cited by 13 publications

(8 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the allergens found in the Poaceae species plant, pollen-specific maize proteins showed significant sequence homology with other specific proteins from nomocot and dicot plants [15,16]. Some of these allergens were only expressed in pollen, not in vegetable and floral tissue [17].…”

Section: Resultsmentioning

confidence: 97%

Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens

et al. 2000

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 97%

Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens

et al. 2000

View full text Add to dashboard Cite

“…Although SPT data might be in favor of cross-reactivity between maize and rice pollen, this trend was not confirmed by immunoblot results that showed more reactivities to maize pollen (85%) than to rice pollen (40%). Since allergen families are shared between maize and rice as well as between wild and cultivated grass pollen, cross-reactivities were expected [ 16 , 17 , 24 , 32 – 34 ]. However, some specific sensitization can also occur in maize pollen allergy and rather associated to occupational allergy [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…Ten allergens have been described in maize and 7 of them, belonging to the same protein families, were also found in rice pollen. The major allergens β-expansins (groups 1-3 of grass pollen allergens) [ 20 – 22 ], profilin (group 12) [ 23 ] and polygalacturonase (group 13) [ 24 ] were characterized both in maize and rice pollen.…”

Section: Introductionmentioning

confidence: 99%

High IgE sensitization to maize and rice pollen in the highlands of Madagascar

Ramavovololona¹,

Sénéchal²,

Andrianarisoa³

et al. 2014

Pan Afr Med J

View full text Add to dashboard Cite

IntroductionMaize and rice are two crops constituting the main food supply in many under-developed and developing countries. Despite the large area devoted to the culture, the sensitization to the pollen from these plants is reported to be low and often considered as an occupational allergy.MethodsSixty five Malagasy pollen allergic patients were clinically and immunochemically investigated with regard to maize and rice pollen allergens. Pollen extracts were electrophoretically separated in 1 and 2 dimensions and IgE and IgG reactivities detected upon immunoblotting.ResultsWhen exploring the sensitization profile of Malagasy allergic patients to maize and rice pollen, it appears that a high proportion of these patients consulting during grass pollinating season were sensitized to both pollen as revealed by skin prick testing (62 vs. 59%) and IgE immunoblotting (85 vs. 40%). Several clinically relevant allergens were recognized by patients’ serum IgE in maize and rice pollen extracts.ConclusionThe high levels of maize and rice pollen sensitization should be related, in this tropical region, to a specific environmental exposure including i) a proximity of the population to the allergenic sources and ii) a putative exacerbating effect of a highly polluted urban atmosphere on pollen allergenicity. Cross-reactivities between wild and cultivated grasses and also between rice and maize pollen are involved as well as some specific maize sensitizations. The presence of dense urban and peri-urban agriculture, in various African regions and worldwide, could be a high environmental risk factor for people sensitive to maize pollen.

show abstract

“…The reason for this might be Zea m 13 and homologous proteins which are present in both maize pollen and maize seed [7]. …”

Section: Introductionmentioning

confidence: 99%

Maize pollen is an important allergen in occupationally exposed workers

Oldenburg

Petersen

Baur

2011

Journal of Occupational Medicine and Toxicology

View full text Add to dashboard Cite

BackgroundThe work- or environmental-related type I sensitization to maize pollen is hardly investigated. We sought to determine the prevalence of sensitization to maize pollen among exposed workers and to identify the eliciting allergens.MethodsIn July 2010, 8 out of 11 subjects were examined who were repeatedly exposed to maize pollen by pollinating maize during their work in a biological research department. All 8 filled in a questionnaire and underwent skin prick testing (SPT) and immune-specific analyses.Results5 out of the 8 exposed subjects had repeatedly suffered for at least several weeks from rhinitis, 4 from conjunctivitis, 4 from urticaria, and 2 from shortness of breath upon occupational exposure to maize pollen. All symptomatic workers had specific IgE antibodies against maize pollen (CAP class ≥ 1). Interestingly, 4 of the 5 maize pollen-allergic subjects, but none of the 3 asymptomatic exposed workers had IgE antibodies specific for grass pollen. All but one of the maize pollen-allergic subjects had suffered from allergic grass pollen-related symptoms for 6 to 11 years before job-related exposure to maize pollen. Lung function testing was normal in all cases. In immunoblot analyses, the allergenic components could be identified as Zea m 1 and Zea m 13. The reactivity is mostly caused by cross-reactivity to the homologous allergens in temperate grass pollen. Two sera responded to Zea m 3, but interestingly not to the corresponding timothy allergen indicating maize-specific IgE reactivity.ConclusionThe present data suggest that subjects pollinating maize are at high risk of developing an allergy to maize pollen as a so far underestimated source of occupational allergens. For the screening of patients with suspected maize pollen sensitization, the determination of IgE antibodies specific for maize pollen is suitable.

show abstract

Expression of Zm13, a pollen specific maize protein, in Escherichia coli reveals IgE‐binding capacity and allergenic potential

Cited by 13 publications

References 40 publications

Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens

Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens

High IgE sensitization to maize and rice pollen in the highlands of Madagascar

Maize pollen is an important allergen in occupationally exposed workers

Contact Info

Product

Resources

About