Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences

Geng, Yuke; Pang, Bao-sen; Hao, Chenyang; Tang, Saijun; Zhang, Xueyong; Tian, Li

doi:10.1371/journal.pone.0105363

Cited by 27 publications

(30 citation statements)

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“…The high homology of OsGS5 and TaGS5‐3A suggests a similar function (Figure S1). To confirm this, we transformed TaGS5‐3A‐T and TaGS5‐3A‐G into rice under control of an endosperm‐specific promoter cloned from the wheat HMW‐GS gene 1Bx7 OE (Geng et al ., ). Grain trait analyses of two transgenic lines showed that T 2 TaGS5‐3A (OE) lines (transgenic lines overexpressing TaGS5‐3A ) had increased grain size and TKW compared to wild type (Figure , Table ).…”

Section: Resultsmentioning

confidence: 97%

“…We overexpressed TaGS5-3A-T and TaGS5-3A-G driven by an endosperm-specific vector in rice. To obtain the endospermspecific vector, we replaced the rice actin 1 promoter in a modified pCAMBIA2300 vector with an endosperm-specific promoter that was cloned from the wheat high molecular weight glutenin subunit (HMW-GS) 1Bx7 OE (Geng et al, 2014). The coding sequences of the two alleles were introduced into the vector under control of the 1Bx7 OE promoter.…”

Section: Transformation Of Tags5-3a In Ricementioning

confidence: 99%

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TaGS5‐3A, a grain size gene selected during wheat improvement for larger kernel and yield

Tian

Hao

et al. 2015

Plant Biotechnology Journal

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.com (XC) and Tel/fax +86 10 82106695; email zhangxueyong@caas.cn (XZ)) † These authors contributed equally to this work.Keywords: TaGS5, Serine carboxypeptidase, Grain size, Breeding selection, Triticum spp.. SummaryGrain size is a dominant component of grain weight in cereals. Earlier studies have shown that OsGS5 plays a major role in regulating both grain size and weight in rice via promotion of cell division. In this study, we isolated TaGS5 homoeologues in wheat and mapped them on chromosomes 3A, 3B and 3D. Temporal and spatial expression analysis showed that TaGS5 homoeologues were preferentially expressed in young spikes and developing grains. Two alleles of TaGS5-3A, TaGS5-3A-T and TaGS5-3A-G were identified in wheat accessions, and a functional marker was developed to discriminate them. Association analysis revealed that TaGS5-3A-T was significantly correlated with larger grain size and higher thousand kernel weight. Biochemical assays showed that TaGS5-3A-T possesses a higher enzymatic activity than TaGS5-3A-G. Transgenic rice lines overexpressing TaGS5-3A-T also exhibited larger grain size and higher thousand kernel weight than TaGS5-3A-G lines, and the transcript levels of cell cycle-related genes in TaGS5-3A-T lines were higher than those in TaGS5-3A-G lines. Furthermore, systematic evolution analysis in diploid, tetraploid and hexaploid wheat showed that TaGS5-3A underwent strong artificial selection during wheat polyploidization events and the frequency changes of two alleles demonstrated that TaGS5-3A-T was favoured in global modern wheat cultivars. These results suggest that TaGS5-3A is a positive regulator of grain size and its favoured allele TaGS5-3A-T exhibits a larger potential application in wheat high-yield breeding.

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Section: Resultsmentioning

confidence: 97%

Section: Transformation Of Tags5-3a In Ricementioning

confidence: 99%

TaGS5‐3A, a grain size gene selected during wheat improvement for larger kernel and yield

Tian

Hao

et al. 2015

Plant Biotechnology Journal

Self Cite

182

134

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“…Fourth, we demonstrated, by ectopic expression in Arabidopsis, that TaGAMyb activates the transcription of TaGLU‐1Dy via binding to its promoter. We provided direct evidence that TaGAMyb regulates the expression of HMW‐GS by binding its promoter, by a cis ‐element previously identified in the promoters of wheat HMW‐GS genes (Geng et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Recent work also analyzed the HMW‐GS promoters in silico (Geng et al ., ; Ravel et al ., ). The 5′ proximal flanking regions of HMW‐GS promoters contain five common motifs, including DOF (DNA‐binding with one finger) recognition sites (5′‐TGTAAAG‐3′), bZIP recognition sites such as the GCN4‐like motifs (5′‐ATGAG/CTCAT‐3′) and the G‐box motif (5′‐TTACGTGG‐3′), MYB recognition sites (5′‐AACAAC‐3′), VP1 recognition sites, and basal promoter elements, which are conserved in the promoters of other seed storage protein genes (Geng et al ., ). Among these conserved elements, the G‐box like element in the HMW‐GS 1Dx5 promoter has been reported to be necessary and sufficient for its expression in transient assays in maize endosperm (Norre et al ., ).…”

Section: Introductionmentioning

confidence: 98%

The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

et al. 2015

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SUMMARYGlutenin proteins in wheat (Triticum aestivum L.) flour confer unique viscoelastic properties to dough products and, therefore, the concentration and composition of the glutenin proteins determine its end-use value. However, the mechanisms governing the glutenin gene expression remain elusive. In this study, we report that wheat TaGAMyb activates the high-molecular-weight glutenin subunit genes (TaGLU) through recruiting the histone acetyltransferase GCN5. By sequencing the promoters of TaGLU-1 genes from 40 modern wheat cultivars, we identified eight types of TaGAMyb binding motifs and verified these by electrophoretic mobility shift assays. The number of TaGAMyb binding motifs in TaGLU-1 genes is correlated with the abundance of glutenin in different cultivars. Chromatin immunoprecipitation plus polymerase chain reaction (ChIP-PCR) analysis reveals that TaGCN5 directly targets the promoters of TaGLU-1 genes in wheat endosperm. We find that TaGAMyb physically interacts with the wheat histone acetyltransferase TaGCN5 and also interacts with Arabidopsis thaliana AtGCN5. TaGAMyb ectopically expressed in Arabidopsis binds to the TaGLU-1Dy promoter on a TaGLU-1Dy transgene and activates its expression. AtGCN5 also targets the TaGLU-1Dy transgene and is involved in the establishment of acetylation at H3K9 and H3K14. These results demonstrate that TaGAMyb plays a dual role in activating expression of glutenin gene by directly binding to the TaGLU promoter and by recruiting GCN5 to modulate histone acetylation during wheat endosperm development.

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“…To drive the strong expression of TubZIP28 (TRIUR3_00571) specifically in developing endosperms, the promoter of high‐molecular‐weight glutenin subunit (HMW‐GS) gene Glu‐1Bx14 (Geng et al , ) was used in the overexpression construct. The overexpression construct was transformed into immature embryos of ‘Kenong 199’ by the particle bombardment method according to Shan et al () to generate overexpression wheat of TubZIP28 .…”

Section: Methodsmentioning

confidence: 99%

TubZIP28, a novel bZIP family transcription factor from Triticum urartu, and TabZIP28, its homologue from Triticum aestivum, enhance starch synthesis in wheat

et al. 2020

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Summary Starch in wheat grain provides humans with carbohydrates and influences the quality of wheaten food. However, no transcriptional regulator of starch synthesis has been identified first in common wheat (Triticum aestivum) due to the complex genome. Here, a novel basic leucine zipper (bZIP) family transcription factor TubZIP28 was found to be preferentially expressed in the endosperm throughout grain‐filling stages in Triticum urartu, the A genome donor of common wheat. When TubZIP28 was overexpressed in common wheat, the total starch content increased by c. 4%, which contributed to c. 5% increase in the thousand kernel weight. The grain weight per plant of overexpression wheat was also elevated by c. 9%. Both in vitro and in vivo assays showed that TubZIP28 bound to the promoter of cytosolic AGPase and enhanced both the transcription and activity of the latter. Knockout of the homologue TabZIP28 in common wheat resulted in declines of both the transcription and activity of cytosolic AGPase in developing endosperms and c. 4% reduction of the total starch in mature grains. To the best of our knowledge, TubZIP28 and TabZIP28 are transcriptional activators of starch synthesis first identified in wheat, and they could be superior targets to improve the starch content and yield potential of wheat.

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Expression of Wheat High Molecular Weight Glutenin Subunit 1Bx Is Affected by Large Insertions and Deletions Located in the Upstream Flanking Sequences

Cited by 27 publications

References 50 publications

TaGS5‐3A, a grain size gene selected during wheat improvement for larger kernel and yield

TaGS5‐3A, a grain size gene selected during wheat improvement for larger kernel and yield

The wheat transcription factor TaGAMyb recruits histone acetyltransferase and activates the expression of a high‐molecular‐weight glutenin subunit gene

TubZIP28, a novel bZIP family transcription factor from Triticum urartu, and TabZIP28, its homologue from Triticum aestivum, enhance starch synthesis in wheat

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