Expression of VEGF-C/-D and lymphangiogenesis in salivary adenoid cystic carcinoma

Stárek, Ivo; Salzman, Richard; Kučerová, Ladislava; Skálová, Alena; Hauer, Lukáš

doi:10.1016/j.prp.2015.07.001

Cited by 11 publications

(3 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, in the Fujita's 26 series of 29 salivary adenoid cystic carcinomas only sparse peritumourous but no intratumorous lymphatics were seen, implying this entity was incapable of stimulating their formation. Their findings are in contradiction with our previous study demonstrating the presence and prognostic relevance of the latter channels in adenoid cystic carcinomas 27 . Soares et al 28 assumed that intratumorous lymphatics represented an additional pathway increasing the metastatic potential in widely invasive carcinoma ex pleomorphic adenoma.…”

Section: Introductioncontrasting

confidence: 99%

“…Information regarding the expression of the major lymphangiogenic factors in salivary gland tumours is very limited. In our two previous papers, we demonstrated moderate to strong VEGF-C and VEGF-D immunoreactivity in all cell types of salivary adenoid cystic carcinomas 27 and expression of only the latter cytokine in the cells of epithelial as well as mesenchymal component of pleomorphic adenomas 44 . Moreover, moderate expression of primarily haemangiogenic VEGF(A), known to cooperate with VEGF-C in the regulation of lymphangiogenesis 59 was reported in the epithelial and some stromal lymphoid cells of WTs by Faur et al 29 and Nakamura et al…”

Section: Discussionmentioning

confidence: 53%

See 1 more Smart Citation

Comparison of lymphatic vessel density and expression of VEGF-C and VEGF-D lymphangiogenic factors in Warthin's tumours and oncocytic adenomas

Hoza¹,

Salzman²,

Bakaj³

et al. 2018

BIOMED PAP

Self Cite

View full text Add to dashboard Cite

Objectives.To compare the density of lymphatic vessels and VEGF-C and VEGF-D expression in Warthin's tumours (WTs) and oncocytic adenomas (OCAs). Methods. Twenty three WTs and 13 OCAs of the parotid gland were analyzed. Lymphatic vessels were detected using the D2-40 antibody. For evaluation of the intratumour and peritumour lymphatic vessel density (iLVD and pLVD, respectively) the area of greatest vascularisation (hot spots) was chosen, using a ×40 field, and the number of vessels per square millimeter was counted in a ×200 field. The staining intensity for VEGF-C and VEGF-D immunoreaction in the tumour cells was graded from 0 to 3. Results. The mean iLVD and pLVD values in WTs was 4.7 (range 1-8) and 6.9 (range 3-10), those in the OCAs 1.0 (range 0-3) and 5.8 (range 2-8), respectively. The differences in the iLVD, but not pLVD between the two tumour groups were statistically significant. In both entities, the pLVD markedly outnumbered the iLVD. The intratumour vessels in the WTs were present exclusively in the lymphoid stroma. In the group of 23 WTs, 13 (56.6%), 17 (73.9%) and 10 (43.4%) samples revealed positive VEGF-C, VEGF-D and both immunoreactions, respectively. 10 of 13 (77%) cases revealed VEGF-D immunoreaction and in none of them was the VEGF-C reaction present. Conclusion. The tumours had a comparable high density of peritumorous lymphatic network. However, WTs markedly differed from OCAs in the number of the intratumorous vessels. These were abundant solely in the stroma of WT, while practically lacking in the neoplastic epithelium of the WT and relatively rare in OCAs. We suggest that homeostasis in both entities is mediated mainly by peritumorous lymphatics. The lymphatic drainage in WTs is also fostered exclusively by stromal lymphatics, whereas in stroma poor OCAs by the vessels present in their neoplastic epithelium. We also believe that WTs stimulate proliferation of pre-existing lymphatic capillaries by means of the paracrine secretion of VEGF-C and VEGF-D in the neoplastic as well as reactive stromal cells, while in the OCAs only the latter factor takes part in their lymphangiogenesis.

show abstract

Section: Introductioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 53%

Comparison of lymphatic vessel density and expression of VEGF-C and VEGF-D lymphangiogenic factors in Warthin's tumours and oncocytic adenomas

Hoza¹,

Salzman²,

Bakaj³

et al. 2018

BIOMED PAP

Self Cite

View full text Add to dashboard Cite

show abstract

“…Stárek et al [42] demonstrated that the majority (17 of 20) of adenoid cystic carcinoma revealed concurrently positivity to VEGF-C and VEGF-D, with scores exceeding those determined in the normal salivary gland acini. So, probably lymphangiogenic growth factors were produced by the adenoid cystic carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

Correlation of intratumoral lymphatic microvessel density, vascular endothelial growth factor C and cell proliferation in salivary gland tumors

et al. 2016

View full text Add to dashboard Cite

Lymphatic dissemination is one of the most important pathways for metastasis in many solid tumors, including head and neck carcinomas. The lymphatic growth of cancer has been used as a significant independent adverse prognostic factor and provides information about tumor progression. Salivary gland tumors present different prognoses and have the ability to develop metastases; however, this information regarding the lymphatic spread is scarce. This paper quantifies the lymphatic microvessel density (LMD) in benign and malignant salivary gland tumors and analyzes the relationship between LMD and tumor expression of vascular endothelial growth factors C (VEGF-C) and the proliferative index. The results show that there is no correlation between LMD, VEGF-C and the proliferative index in the majority of salivary gland tumors analyzed, apart from polymorphous low-grade carcinoma which exhibits statistical correlation between LMD and the proliferative index (p < 0.05). This correlation probably does not indicate a poor prognosis for this PLGA, since this is a low metastasizing carcinoma of the salivary glands. Different from other solid tumors, such as breast or prostatic carcinomas, there is no correlation between VEGF-C and LMD in salivary gland tumors, and so these traits are not able to estimate the metastatic risk or the prognosis of these tumors.

show abstract

Study of MYB-NFIB chimeric gene expression, tumor angiogenesis, and proliferation in adenoid cystic carcinoma of salivary gland

Ono

Okada

2017

Odontology

View full text Add to dashboard Cite

Adenoid cystic carcinoma (ACC) is one of the common malignant tumors in salivary glands, and the clinical prognosis is poor with frequent distant metastasis which may lead to death. Expression of the MYB-NFIB chimeric gene in ACC has been reported recently. MYB is an oncogene with transcription regulating functions, and NFIB encodes nuclear transcription factor although detailed functions are unknown. This study investigated whether MYB-NFIB chimeric gene expression affects tumor angiogenesis and proliferation in salivary gland ACC. In 26 salivary gland ACC cases, MYB-NFIB chimeric gene expression was analyzed by RT-PCR and direct sequencing. Immunohistochemical studies for CD31, vascular endothelial growth factor (VEGF) and Ki-67 were performed. Tumor angiogenesis was evaluated by blood vessel (CD31-positive) density and tumor proliferation by Ki-67 labeling index, and the relationship with MYB-NFIB chimeric gene expression was analyzed. MYB-NFIB chimeric gene expression was detected in nine of 26 ACC cases. Blood vessel density was significantly higher in chimeric gene-expressing cases compared to non-expressing cases. VEGF score tended to be higher in chimeric gene-expressing cases than in non-expressing cases, while Ki-67 labeling index was not significantly different. The number of chimeric gene-expressing cases increased with age, peaking in the sixties age group and declining thereafter, while the number of non-expressing cases increased with age continuously. In ACC, blood vessel density was significantly higher in MYB-NFIB chimeric gene-expressing cases compared to non-expressing cases, which may be due to higher VEGF production capability. MYB-NFIB chimeric gene expression may also be related to the onset age of ACC.

show abstract

Expression of VEGF-C/-D and lymphangiogenesis in salivary adenoid cystic carcinoma

Cited by 11 publications

References 59 publications

Comparison of lymphatic vessel density and expression of VEGF-C and VEGF-D lymphangiogenic factors in Warthin's tumours and oncocytic adenomas

Comparison of lymphatic vessel density and expression of VEGF-C and VEGF-D lymphangiogenic factors in Warthin's tumours and oncocytic adenomas

Correlation of intratumoral lymphatic microvessel density, vascular endothelial growth factor C and cell proliferation in salivary gland tumors

Study of MYB-NFIB chimeric gene expression, tumor angiogenesis, and proliferation in adenoid cystic carcinoma of salivary gland

Contact Info

Product

Resources

About