Expression of Vascular Endothelial Growth Factor in Invasive Ductal Carcinoma of the Breast and the Relation to Angiogenesis and p53 and HER-2/neu Protein Expression

Lee, Ji Shin; Kim, Hyung Seok; Jung, Jong Jae; Kim, Young Bog; Lee, Min Cheol; Park, Chang Soo

doi:10.1097/00129039-200212000-00001

Cited by 16 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6 Several studies have reported that VEGF expression ranged from 80% to 95% in human breast cancer, and this expression rate was confirmed in our series of 201 cases of invasive carcinomas with 98%. In agreement with previous observations by Lee at al, 11 we found a strong expression at tumor margins. Like other groups, 12,13 we found that VEGF expression was inversely related to the ER level.…”

Section: Discussionsupporting

confidence: 94%

“…Although it has been suggested that the oncogenic properties of HER2 might be mediated by stimulation of tumor angiogenesis and upregulation of VEGF, 1,2,14 our study, corresponding to the results of other investigators, 11,12 could not show a significant correlation between VEGF and HER2. The fact that VEGF expression is subject to a number of other regulatory mechanisms may explain why a large proportion of patients with tumors that did not overexpress HER2 do express VEGF (97.5%), and why there is no correlation between the expression of HER2 and VEGF among patients with HER2-overexpressing tumors and detectable VEGF expression.…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

HER2 Is Unlikely to Be Involved in Directly Regulating Angiogenesis in Human Breast Cancer

Vogl¹,

Bartel²,

Dietze³

et al. 2006

Applied Immunohistochemistry &Amp; Molecular Morphology

View full text Add to dashboard Cite

Angiogenesis is a fundamental component of oncogenesis. Angiogenic factors such as vascular endothelial growth factor (VEGF) and platelet derived-endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) are generated from tumor cells to provide tumor growth and are thought to be regulated via the HER2 oncogene, whose amplification is the most common genetic alteration in breast cancer. The present study aimed to evaluate the immunoreactivity of angiogenic factors (VEGF, PD-ECGF/TP) and microvessel density (MVD) via epidermal growth factor receptor (EGFR) and HER2, and to correlate their expression with clinicopathologic features. Two hundred one invasive human breast cancer specimens were tested immunohistochemically for the expression of these proteins. In addition, MVD was examined using computerized image analysis. VEGF could be an additional interesting prognostic variable, as it was significantly associated with tumor grade (P=0.002), stage (P=0.018), and negative estrogen receptor status (P=0.011). EGFR was significantly related to invasive ductal carcinoma (P=0.030), tumor grade (P=0.009), VEGF expression (P=0.013), PD-ECGF/TP expression (P=0.024), and MVD (P=0.050). The finding that VEGF is not correlated to MVD does not rule out a crucial role of VEGF as a key factor in angiogenesis. HER2 could not be correlated to MVD, VEGF expression, or PD-ECGF/TP expression, indicating that this factor is unlikely to be involved in directly regulating angiogenesis, whereas the significant correlations between EGFR and histologic tumor type, tumor grade, the angiogenic factors VEGF and PD-ECGF/TP, and MVD point out that EGF is the major modulating growth factor for angiogenesis in breast cancer.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 83%

HER2 Is Unlikely to Be Involved in Directly Regulating Angiogenesis in Human Breast Cancer

Vogl¹,

Bartel²,

Dietze³

et al. 2006

Applied Immunohistochemistry &Amp; Molecular Morphology

View full text Add to dashboard Cite

show abstract

“…19 Tumor VEGF expression correlates significantly with angiogenic activity and survival in human breast cancer. 6,20 In our patients we observed an inverse correlation between VEGF and tumor size and nodal status ( Table 2). Although this may seem surprising, the relation between VEGF and these conventional prognostic factors is not well defined, and the results of different studies have been discordant.…”

Section: Discussionsupporting

confidence: 55%

Elevated Serum Levels of Vascular Endothelial Growth Factor Are Associated With Tumor-Associated Macrophages in Primary Breast Cancer

et al. 2006

View full text Add to dashboard Cite

We analyzed serum and tumor samples from 133 patients with operable primary breast cancer to determine the possible relationship between presurgery and postsurgery circulating serum vascular endothelial growth factor (VEGF) levels and tumor-associated macrophage (TAM) numbers, tumor VEGF expression, and other immunohistochemical parameters. A significant positive correlation was observed between the number of TAM and postsurgery circulating VEGF values (P < .05). Moreover, patients with a p53+ tumor had higher postsurgery serum VEGF levels than those with a p53- tumor (P < .05), and tumor p53 overexpression correlated significantly with TAM number (P = .007). We observed no significant association between serum values and tumor VEGF expression. Although the macrophage index was higher in VEGF+ than in VEGF- tumors, the differences were not statistically significant. Our data show a positive interrelation between high circulating VEGF levels, the number of TAM, and p53 overexpression, a relationship that might have an important role in the enhanced angiogenesis processes in breast cancer.

show abstract

“…However, mixed results were observed. Considering conventional histological prognostic factors, increased microvessel density had been reported in a number of studies to correlate with larger tumor size (Bolat et al, 2006;Thielemann et al, 2008;Thorat et al, 2009), higher tumor grade (Lee et al, 2002;Valkovic et al, 2002;Bolat et al, 2006;Vogl et al, 2006;Zhou et al, 2006;Thorat et al, 2009) and lymph node metastasis (Weidner et al, 1991;Valkovic et al, 2002;Lee et al, 2002;Bolat et al, 2006). In other studies, however, relationships with these histological prognostic factors could not be validated (Miliaras et al, 1995;Gasparini et al, 1996;Acenero et al, 1998;Fridman et al, 2000;Koukourakis et al, 2003;Ludovini et al, 2003).…”

Section: Microvessel Density In Invasive Breast Ductal Carcinoma Nosmentioning

confidence: 99%

Characteristics of Invasive Breast Ductal Carcinoma, NOS, Diagnosed in a Tertiary Institution in the East Coast of Malaysia with a Focus on Tumor Angiogenesis

Ch’ng

Sharif²,

Jaafar³

2012

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Background: Prognosis of breast cancer depends on classic pathological factors and also tumor angiogenesis. This study aimed to evaluate the clinicopathological factors of breast cancer in a tertiary centre with a focus on the relationship between tumor angiogenesis and clinicopathological factors. Methods: Clinicopathological data were retrieved from the archived formal pathology reports for surgical specimens diagnosed as invasive ductal carcinoma, NOS. Microvessels were immunohistochemically stained with anti-CD34 antibody and quantified as microvessel density. Results: At least 50% of 94 cases of invasive breast ductal carcinoma in the study were advanced stage. The majority had poor prognosis factors such as tumor size larger than 50mm (48.9%), positive lymph node metastasis (60.6%), and tumor grade III (52.1%). Higher percentages of estrogen and progesterone receptor negative cases were recorded (46.8% and 46.8% respectively). Her-2 overexpression cases and triple negative breast cancers constituted 24.5% and 22.3% respectively. Significantly higher microvessel density was observed in the younger patient age group (p=0.012). There were no significant associations between microvessel density and other clinicopathological factors (p>0.05). Conclusions: Majority of the breast cancer patients of this institution had advanced stage disease with poorer prognostic factors as compared to other local and western studies. Breast cancer in younger patients might be more proangiogenic.

show abstract

Expression of Vascular Endothelial Growth Factor in Invasive Ductal Carcinoma of the Breast and the Relation to Angiogenesis and p53 and HER-2/neu Protein Expression

Cited by 16 publications

References 26 publications

HER2 Is Unlikely to Be Involved in Directly Regulating Angiogenesis in Human Breast Cancer

HER2 Is Unlikely to Be Involved in Directly Regulating Angiogenesis in Human Breast Cancer

Elevated Serum Levels of Vascular Endothelial Growth Factor Are Associated With Tumor-Associated Macrophages in Primary Breast Cancer

Characteristics of Invasive Breast Ductal Carcinoma, NOS, Diagnosed in a Tertiary Institution in the East Coast of Malaysia with a Focus on Tumor Angiogenesis

Contact Info

Product

Resources

About