Expression of two markers of germinal center T cells (SAP and PD-1) in angioimmunoblastic T-cell lymphoma

Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

“…These authors also examined the follicular center helper T-cellassociated marker SAP in six mycosis fungoides cases but did not detect significant expression in any. 31 In contrast to these studies, Cetinozman et al 34 identified infrequent expression of PD-1 in mycosis fungoides observing reactivity in only 2 of 21 (9.5%) of cases. The reason for the reported differences in the frequency of PD-1 expression in mycosis fungoides is unclear but may relate in part to specific reagents used and the stage of disease examined.…”

“…They did not evaluate Sezary syndrome cases. Roncador et al 31 evaluated PD-1 in a wide array of lymphomas including mycosis fungoides and noted expression in 5/9 mycosis fungoides. These authors also examined the follicular center helper T-cellassociated marker SAP in six mycosis fungoides cases but did not detect significant expression in any.…”

“…15,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] These include angioimmunoblastic T-cell lymphoma, peripheral T-cell lymphoma with a follicular growth pattern, primary cutaneous CD4( þ ) small/ medium-sized pleomorphic T-cell lymphoma and, occasionally, peripheral T-cell lymphoma, not otherwise specified and anaplastic large cell lymphoma. 15,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] Clinically, primary cutaneous CD4( þ ) small/ medium-sized pleomorphic T-cell lymphoma and angioimmunoblastic T-cell lymphoma present or may present with primary cutaneous involvement. 20,[36][37][38][39][40] Other CD4( þ ) T-cell lymphomas with primary cutaneous manifestations include mycosis fungoides and its variants including Sezary syndrome, adult T-cell leukemia/lymphoma and primary cutaneous CD30 þ lymphoproliferative disorders.…”

Lymphocyte-rich classical Hodgkin's lymphoma: distinctive tumor and microenvironment markers

“…The presence and histological distribution of follicular T cells is becoming more widely considered as useful for exploring the pathogenesis of several lymphoproliferative disorders. 5,17,18 Follicular T-cell rosettes surrounding tumoral lymphocytic and/or histiocytic Reed-Sternberg cell variants within nodular areas of follicular dendritic cell meshworks are considered a diagnostic feature of nodular lymphocyte-predominant Hodgkin's lymphoma. These cells have an immunophenotype similar to that of intrafollicular T cells, that is, they are CD57 positive and PD-1 positive and co-express bcl-6.…”

“…Double immunohistochemical or immunofluorescence staining with PD-1 and CD30, bcl-6 and CD30, OCT.2 and CD30, and BOB.1 and CD30 was also carried out in reactive tonsil, reactive lymph nodes, and in six lymphocyterich classical Hodgkin's lymphoma cases, in which preserved residual germinal centers could be easily identified, using protocols described earlier. 5,6 Immunofluorescence slides were examined on a Nikon E800 Eclipse fluorescence microscope (Nikon, Kingston-upon-Thames, UK) equipped for epifluorescence, and images were captured with an Axiocam charge-coupled device (CCD) camera (Zeiss, Jena, Germany) and Axiovision software (Imaging Associates, Bicester, UK), and adjusted using Photoshop software (Adobe, San Jose, CA, USA).…”