Expression of Tumor Necrosis Factor Alpha-Induced Protein 3 mRNA in Peripheral Blood Mononuclear Cells Negatively Correlates with Disease Severity in Psoriasis Vulgaris

Abstract

e Psoriasis vulgaris is considered a chronic inflammatory disease, but its immunopathogenesis has not been well understood. The tumor necrosis factor alpha-induced protein 3 (TNFAIP3) gene functions in negative-feedback regulation of inflammation, and its single nucleotide polymorphism is associated with psoriasis. However, the relationship between the expression level of the TNFAIP3 gene in immune cells and psoriasis is not known so far. In the present study, TNFAIP3 mRNA expression levels in peripheral blood mononuclear cells from 44 patients with psoriasis vulgaris and 30 healthy controls were determined using realtime reverse transcription-PCR analysis. We found that expression of TNFAIP3 mRNA in all patients negatively correlated with the psoriatic area and severity index (PASI) (r ‫؍‬ ؊0.5126; P ‫؍‬ 0.0004) as well as with the percentage of body surface area affected by psoriasis (r ‫؍‬ ؊0.5013; P ‫؍‬ 0.0005). Patients were divided into mild and severe groups based on the mean PASI score. Expression of TNFAIP3 mRNA in the mild group was higher than that in the severe group (P ‫؍‬ 0.0064). Moreover, compared with that in healthy controls, the expression of TNFAIP3 mRNA in the mild group was significantly upregulated (P ‫؍‬ 0.0004), but the expression of TNFAIP3 mRNA in the severe group was not. These results suggest that the expression level of TNFAIP3 plays an important role in the pathology of psoriasis vulgaris and that the loss of upregulation of TNFAIP3 expression may contribute to the severity of psoriasis vulgaris.

Genome Wide Transcriptome Analysis of Dendritic Cells Identifies Genes with Altered Expression in Psoriasis

“…Of note, TNFAIP3 deficient mice die early after the birth due to serious multi-organ inflammation [32] and the targeting of TNFAIP3 in different cell types was shown to be associated with chronic inflammation and autoimmunity [33–35]. Furthermore the expression of TNFAIP3 in peripheral blood mononuclear cells of psoriatic patients negatively correlates with disease severity [36], and TNFAIP3 gene has been identified as a susceptibility locus for several autoimmune disorders, including psoriasis [37]. TNFAIP8 is also an essential negative regulator of inflammation [18] and is also involved in the protection against apoptosis [38].…”

TheTNFAIP3polymorphism rs610604 both associates with the risk of psoriasis vulgaris and affects the clinical severity

“…The TNFAIP3 gene encodes A20, a TNF‐α‐inducible zinc finger protein. A20 plays an important role in the negative feedback regulation of NF‐κB (nuclear factor kappa light‐chain enhancer of activated B cells) signalling, as a negative immunoregulatory protein . Moreover, A20 also regulates TNF‐induced apoptosis and acts at multiple steps in the NF‐κB signalling pathway .…”

Upregulation of Tumor Necrosis Factor Alpha-Induced Protein 3 mRNA in Mild Psoriasis Vulgaris

“…; Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China b I t was with the utmost interest that we read the recent publication by Jiang et al (1). The paper describes tumor necrosis factor alpha-induced protein 3 (TNFAIP3) mRNA expression levels in peripheral blood mononuclearcellsfrom44patientswithpsoriasisvulgarisnegativelycor-related with the psoriatic area and severity index (PASI) and with the percentageofbodysurfacearea(BSA)affectedbypsoriasis.Furthermore, in comparison with the severe group and 30 healthy controls, expression of TNFAIP3 mRNA in the mild group was significantly upregulated.…”