Expression of trypsin-like activity by the genera Corynebacterium and Actinomyces in canine periodontitis

Abstract

Trypsin-like activity (TLA), clinical parameters and TLA-postitive bacteria were examined in periodontitis and healthy sites in dogs. TLA was markedly higher in periodontitis than at healthy sites. There was good correlation between TLA positivity and severity of periodontal disease. The proportions of TLA-positive bacteria to total isolates in periodontitis and healthy sites were 21.1% and 2.1%, respectively. Among TLA-positive bacteria in periodontitis sites, 4.4% showed strong TLA activity, 35.3% showed moderate and 60.3% showed weak activity. In the healthy sites, all the TLApositive bacteria showed weak activity. In all, 90% of the total number of TLA-positve bacteria were identi®ed as belonging to the family Actinomycetaceae; 40% of bacteria belonging to the family Actinomycetaceae were identi®ed as genus Corynebacterium with moderate trypsin-like activity and the remaining 60% were identi®ed as genus Actinomyces with weak activity. Obligately anaerobic bacteria accounted for only 5.9% of the total population of TLA-positive bacteria; they were gram-negative coccobacilli, gram-positve rods and gram-positive cocci. These observations suggested that bacteria in the family Actinomycetaceae may play an important role in periodontitis and that measurement of TLA is a clinically reliable marker for the diagnosis of periodontitis in dogs.

Porphyromonas Gingivalis is Associated With Protease‐Activated Receptor‐2 Upregulation in Chronic Periodontitis

“…Trypsin‐like activity at the GCF was determined using N ‐benzoyl‐ l ‐arginine‐p‐nitroanilide as substrate, following the protocol previously described 13 . Trypsin‐like activity was calculated in trypsin units per milliliter, using a standard serial dilution of trypsin (0.05 to 2 U/mL).…”

Dental Biofilm as Etiological Agent of Canine Periodontal Disease

“…Dental Biofilm as Etiological Agent of Canine Periodontal Disease DOI: http://dx.doi.org /10.5772/intechopen.88305 Species of the genera Actinomyces and Corynebacterium can play the same role in periodontitis in dogs that P. gingivalis plays in periodontitis in humans. This assumption is based on the finding that the ratio of these genera that have trypsin-like activity is increased in areas with periodontitis compared to their ratio in non-periodontal areas and may partly explain the absence of P. gingivalis [43]. Protist organisms, such as Entamoeba gingivalis and Trichomonas tenax, are also present in canine dental biofilm, and correlate with periodontal disease.…”

Protease-activated Receptor-2 (par2) in Human Periodontitis

“…We used an aliquot of the supernatant from the GCF sample to measure the trypsin-like activity with N-benzoyl-L-arginine-pnitroanilide (BApNA) as substrate, following the protocol previously described (Takada and Hirasawa, 2000). Trypsinlike activity was calculated in trypsin units per mL, with a standard reaction mixture with a serial dilution of trypsin (0.05 to 2 U/mL).…”