Expression of Transforming Growth Factor β<sub>1</sub>, β<sub>3</sub>, and Basic Fibroblast Growth Factor in Full‐Thickness Skin Wounds of Equine Limbs and Thorax

Theoret, Christine L.; Barber, Spencer M.; Moyana, Terence; Gordon, John

doi:10.1053/jvet.2001.23341

Cited by 105 publications

(131 citation statements)

References 27 publications

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“…Cross-reaction of the antibodies in these human kits with equine samples had been demonstrated previously, 28,29 or has been assumed on homology between equine and human protein structure. Glycosaminoglycans and DNA Analysis After lyophilizing for 24 h and recording dry weight, the tendon samples were papain digested.…”

Section: Growth Factor Quantificationmentioning

confidence: 73%

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Bosch

Schie

Groot

et al. 2009

Journal Orthopaedic Research

193

241

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Tendon injuries are notorious for their slow and functionally inferior healing. Intratendinous application of platelet-rich plasma (PRP) has been reported to stimulate the repair process of tendon injuries, but there is little conclusive evidence for its effectiveness. A placebo-controlled experimental trial was performed to test the hypothesis that a single intratendinous PRP treatment enhances the quality of tendon repair, as evidenced by improved biochemical, biomechanical, and histological tissue properties. In six horses, tendon lesions were created surgically in the Superficial Digital Flexor Tendons (SDFT) of both front limbs, one of which was treated with PRP and the other with saline. After 24 weeks, the tendons were harvested for biochemical, biomechanical, and histological evaluations. Collagen, glycosaminoglycan, and DNA content (cellularity) was higher in PRP-treated tendons (p ¼ 0.039, 0.038, and 0.034, respectively). The repair tissue in the PRP group showed a higher strength at failure (p ¼ 0.021) and Elastic Modulus (p ¼ 0.019). Histologically, PRP-treated tendons featured better organization of the collagen network (p ¼ 0.031) and signs of increased metabolic activity (p ¼ 0.031). It was concluded that PRP increases metabolic activity and seems to advance maturation of repair tissue over nontreated experimentally induced tendon lesions, which suggests that PRP might be beneficial in the treatment of clinical tendon injuries. ß

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Section: Growth Factor Quantificationmentioning

confidence: 73%

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Bosch

Schie

Groot

et al. 2009

Journal Orthopaedic Research

193

241

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“…A pele íntegra da região da garupa dos equinos mestiços do presente estudo apresentou valores médios muito mais baixos de TGF-β1 (0,94±0,69pg/g) do que os relatados por Theoret et al (2001) na pele sadia dos membros torácicos (MTs) e região torácica (RT) de quatro equinos mestiços com idade entre 2 e 4 anos, que foram respectivamente de 980±396pg/g (MTs) e 1.430±336,0pg/g (RT). Esses valores tão diferentes podem estar relacionados com a idade dos animais.…”

Section: Discussionunclassified

“…Apesar da importância do TFG-β1 em diferentes fases do processo de cicatrização da pele, esse fator de crescimento também está associado com o desenvolvimento de tecido de granulação exuberante devido a sua função pró--fibrótica (Theoret et al 2001, van den Boom et al 2002, Theoret & Wilmink 2013, portanto a sua permanência elevada no final do processo de cicatrização cutânea pode não ser benéfica. Entretanto, ao que parece essa redução na pele é lenta, já que no presente estudo os valores do TGF-β1 permaneceram significativamente altos em relação ao tempo zero (0,94±0,69pg/g), tanto no grupo tratado (24,29±12,67pg/g) como controle (36,31±16,59pg/g), porém não houve a formação de tecido de granulação exuberante.…”

Section: Discussionunclassified

“…Após obtenção, o plasma foi transferido para eppendorfs e armazenado a -80°C até análise. Para a quantificação dos fatores de crescimento no PRP e plasma sanguíneo, as amostras foram descongeladas em temperatura ambiente, e na sequência o TGF-β1 (Quantikine TGF-β1 Imunoassay, DB100B, R&D Systems, LGC Scientific Suplly, Miami, FL, USA) e PDGF-BB (Quantikine PDGF-BB Imunoassay, DBB00, R&D Systems, LGC Scientific Suplly, Miami, FL, USA) foram quantificados utilizando kits humanos que comprovadamente possuem reação cruzada com anticorpos equinos (Theoret et al 2001, Sutter et al 2004, McCarrel & Fortier 2009, Monteiro et al 2009, Textor et al 2011. As concentrações foram determinadas em duplicata, seguindo as instruções do fabricante.…”

Section: Methodsunclassified

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Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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ABSTRACT.-Souza M.V., Pinto J.O., Costa M.M., Santos E.C., Garcia S.L.R. & Oliveira L.L. 2014.[Quantification of growth factors in horse skin treated with platelet-rich plasma.] Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas. Pesquisa Veterinária Brasileira 34(6):599-612. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, MG 36570-000, Brazil. E-mail: msouza@ufv.br Platelet-rich plasma (PRP) is a product derived from total blood centrifugation, rich in bioactive factors, such as growth factors. Despite largely used in healing processes, there is a controversy whether the therapy is effective in promoting skin healing. The objective of this study was to quantify and compare the concentrations of the factors TGF-β1 and PDGF-BB in PRP, blood plasma and skin, at different phases of the healing process of skin treated or not with PRP. Seven healthy crossbred 16 to 17-year-old geldings (16.14±0.63) were used. Three quadrangular-shaped lesions (6.25cm 2 ) were surgically induced into the right and left gluteal regions of all animals. Twelve hours after induction of the wounds, 0.5mL of the PRP was administered in each of the four edges of the wound in one of the gluteal regions (Treated group = TG) randomly chosen. The contralateral region was used as control (CG). The wounds were submitted to daily cleaning with Milli-Q water, and samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, the first carried out immediately after the production of the wound (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days after the lesion was induced. The sixth biopsy (T5) was obtained after complete healing of the skin, which occurred at about day 37 (36.85±7.45, CG; 38.85±6.46, TG). EDTA blood samples were also obtained, at all the times mentioned. Quantification of TGF-β1 and PDGF-BB growth factors on the skin, PRP, and blood plasma was carried out by the ELISA technique. Data were statistically analyzed by the t test, Pearson correlation and regression, at a significance level of 5%. No difference was found between the groups in the values of the two growth factors measured on the skin, at the different times. Also, no correlation was found between the amount of growth factors present in the skin and plasma. On the other hand, a positive correlation was observed between PRP and skin in the treated group, for the growth factors TGF-β1 (r=0.31) and PDGF-BB (r=0.38), as well as between both growth factors present in PRP (r=0.81). Considering the growth factor concentrations at T0, the highest skin values (p<0.05) of TGF-β1, in both groups, occurred at T3 and T5. Higher values (p<0.05) of PDGF-BB occurred at T4 (TG) and T5 (CG). No plasma changes occurred at the concentration of these factors in relation to T0, suggest-1 Recebido em 5 de outubro de 2013.Aceito para publicação em 5 de junho de 2014.

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“…These studies have shown that transforming growth factor -␤ 1 (TGF-␤ 1 ) is an important regulator of scarring and fibrosis both during wound repair as well as in abnormal organ fibrosis (2,4,12,40,51). For example, TGF-␤ 1 expression is increased in abnormal wound healing such as keloids or hypertrophic scars as well as in liver, lung, and renal fibrosis (16,20,21).…”

mentioning

confidence: 99%

TGF-β₁is a negative regulator of lymphatic regeneration during wound repair

Clavin¹,

Avraham²,

Fernandez³

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

186

230

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Expression of Transforming Growth Factor β₁, β₃, and Basic Fibroblast Growth Factor in Full‐Thickness Skin Wounds of Equine Limbs and Thorax

Abstract: The persistence of TGF-beta(1) expression in leg wounds may be related to the development of exuberant granulation tissue in this location, because TGF-beta(1) is profibrotic.

Cited by 105 publications

References 27 publications

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

TGF-β₁is a negative regulator of lymphatic regeneration during wound repair

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Expression of Transforming Growth Factor β1, β3, and Basic Fibroblast Growth Factor in Full‐Thickness Skin Wounds of Equine Limbs and Thorax

Abstract: The persistence of TGF-beta(1) expression in leg wounds may be related to the development of exuberant granulation tissue in this location, because TGF-beta(1) is profibrotic.

Cited by 105 publications

References 27 publications

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Effects of platelet‐rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: A placebo‐controlled experimental study

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

TGF-β1is a negative regulator of lymphatic regeneration during wound repair

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Expression of Transforming Growth Factor β₁, β₃, and Basic Fibroblast Growth Factor in Full‐Thickness Skin Wounds of Equine Limbs and Thorax

TGF-β₁is a negative regulator of lymphatic regeneration during wound repair