Expression of Toll-like receptors and their detection of nuclear self-antigen leading to immune activation in JSLE

Midgley, Angela; Thorbinson, Colin; Beresford, Michael W.

doi:10.1093/rheumatology/ker400

Cited by 21 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TLR3 recognizes dsRNA, and its elevated level in human SLE peripheral blood cells suggests that it may play a key role in IFN signature gene activation22464748. In addition, previous research indicated that TLR3 aggravated lupus nephritis in lupus-prone mice68.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a study of a Mexican population indicated that increased TLR7 copy number correlated with TLR7 mRNA levels and susceptibility to childhood-onset SLE44 although TLR7 copy number variations (CNVs) are infrequent in human SLE45. Other studies indicated that increased expression of TLRs in peripheral blood mononuclear cells and lymphocytes led increased IFN-α expression in SLE patients464748.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Genetic variations in Toll-like receptors (TLRs 3/7/8) are associated with systemic lupus erythematosus in a Taiwanese population

Wang

Chang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

Toll-like receptors (TLRs), as innate immunity sensors, play critical roles in immune responses. Six SNPs of TLR3, TLR7, and TLR8 were genotyped to determine their associations with systemic lupus erythematosus (SLE) and clinical manifestations of SLE. TLR7 SNP rs3853839 was independently associated with SLE susceptibility in females (G vs. C: p = 0.0051). TLR7 rs3853839-G (G vs. C: p = 0.0100) and TLR8 rs3764880-G (recessive model: p = 0.0173; additive model: p = 0.0161) were associated with pericardial effusion in females relative to healthy females. Anti-SSA positive cases were more likely to have the dominant TLR7 rs179010-T allele than normal controls (p = 0.0435). TLR3 rs3775296-T was associated with photosensitivity (p = 0.0020) and anemia (p = 0.0082). The “G-G” haplotype of TLR7 rs3853839 and TLR8 rs3764880 increased risk of SLE in females (age adjusted p = 0.0032). These findings suggest that TLR variations that modify gene expression affect risk for SLE susceptibility, clinical phenotype development, and production of autoantibodies.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Genetic variations in Toll-like receptors (TLRs 3/7/8) are associated with systemic lupus erythematosus in a Taiwanese population

Wang

Chang

et al. 2014

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Gene specific primers for human TLR-7 and TLR-9 and GAPDH genes were selected [17][18][19]. The qPCR reactions were performed in a reaction volume of 20 μL, with 10 μL 2× SensiFAST TM SYBR No-ROX One-Step Mix, 400 nM of each primer, 0.2 μL reverse transcriptase, 0.4 μL RiboSafe RNase inhibitor and 2 μL template.…”

Section: Rna Isolation Reverse Transcription and Quantitative Real-mentioning

confidence: 99%

Quantitative Expression of Toll-Like Receptors TLR-7 and TLR-9 on Peripheral Blood Mononuclear Cells in Leukemias

Morsi¹,

Gharabawy²,

Hamed³

et al. 2016

J Hematol

View full text Add to dashboard Cite

Background: Toll-like receptors (TLRs) are agents of innate and adaptive immunity, involved in tumor activation. The expression and functionality of TLR on leukemic cells have seldom been investigated. The aims of the study were to throw some light on the quantitative expression of TLR-7 and TLR-9 on leukemic cells and to compare it with their expression on mononuclear cells of healthy volunteers.

show abstract

“…Up-regulation of CXCL10 and subsequent CXCR3-positive Th1 cell response is considered crucial for the T-cellmediated destruction of cells that has classically been seen as the hallmark of TIDM (1719). TLR3 activation has been shown to mediate levels of CXCL10 expression in NIT-1 cultured cells transfected with a synthetic double-stranded RNA (dsRNA) (4), and TLR3 mRNA has been shown to be up-regulated in autoimmune disease in human and animal subjects (9, 20). Therefore, we wanted to investigate if there was a difference in expression of CXCL10 between wild-type (TLR3 +/+ ) and TLR3-deficient (TLR3 / ) NOD mice in the pre-diabetic stage.…”

mentioning

confidence: 99%

Laser Capture Microdissection Tailored to Type 1 Diabetes Mellitus Research

et al. 2016

View full text Add to dashboard Cite

RNA isolation from pancreatic islets poses unique challenges. Here, we present a reproducible means of obtaining high-quality RNA from juvenile rodent islets in sufficient quantities for use in ex vivo expression studies. Tissue was extracted from female non-obese diabetic (NOD) toll-like receptor 3 (TLR3)(+/+) and (TLR3)(-/-) mice in the pre-diabetic stage. Samples were frozen in liquid nitrogen, sectioned, fixed in a highly alcoholic solution, and stained with an alcoholic cresyl violet (CV) solution. Rehydration of the fixed sections was minimized. Islets were identified visually and isolated with the Leica LMD6000 laser capture microdissection (LCM) system to yield samples highly enriched in islet RNA. Real time qPCR was performed on the islet cDNA using probes for CXC chemokine ligand 10 (CXCL10), an inflammatory marker that plays a critical role in the pathogenesis of type 1 diabetes mellitus (TIDM). This method represents an improvement over currently described LCM techniques for rodent pancreatic islets and makes feasible expression studies using small amounts of starting tissue without the need for RNA pre-amplification. This has immediate implications for ongoing TIDM studies using the NOD mouse.

show abstract

Expression of Toll-like receptors and their detection of nuclear self-antigen leading to immune activation in JSLE

Cited by 21 publications

References 28 publications

Genetic variations in Toll-like receptors (TLRs 3/7/8) are associated with systemic lupus erythematosus in a Taiwanese population

Genetic variations in Toll-like receptors (TLRs 3/7/8) are associated with systemic lupus erythematosus in a Taiwanese population

Quantitative Expression of Toll-Like Receptors TLR-7 and TLR-9 on Peripheral Blood Mononuclear Cells in Leukemias

Laser Capture Microdissection Tailored to Type 1 Diabetes Mellitus Research

Contact Info

Product

Resources

About