Expression of thermostable β-xylosidase in <i>Escherichia coli</i> for use in saccharification of plant biomass

Aftab, Mohammad; Zafar, Asma; Awan, Ali Raza

doi:10.1080/21655979.2016.1267884

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…It might be because of the reason that with additional rise in incubation time, either the process of product inhibition comes into action due to accretion of glucose and cellobiose, or due to less availability of free cellulose with the passage of time. Results of this research are in accordance with those of Aftab et al (2017) that highest saccharification of sugarcane bagasse was observed after 6 h of incubation using thermophilic cellulases. These alike results can be attributed to the reason that thermophilic cellulases act more energetically and that when an amalgam of enzymes is employed for saccharification, the overall reaction time for hydrolysis is lessened ( Shokrkar and Ebrahimi, 2018 ).…”

Section: Resultssupporting

confidence: 91%

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases

Nawaz

Qadoos²,

Haq³

et al. 2023

Front. Bioeng. Biotechnol.

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Bioethanol is believed to be an influential revolutionary gift of biotechnology, owing to its elevating global demand and massive production. Pakistan is home to a rich diversity of halophytic flora, convertible into bounteous volumes of bioethanol. On the other hand, the accessibility to the cellulosic part of biomass is a major bottleneck in the successful application of biorefinery processes. The most common pre-treatment procedures existent include physicochemical and chemical approaches, which are not environmentally benign. To overcome these problems, biological pre-treatment has gained importance but the drawback is the low yield of the extracted monosaccharides. The current research was aimed at exploring the best pre-treatment method for the bioconversion of halophyte Atriplex crassifolia into saccharides using three thermostable cellulases. Atriplex crassifolia was subjected to acid, alkali and microwave pre-treatments, followed by compositional analysis of the pre-treated substrates. Maximum delignification i.e. 56.6% was observed in the substrate pre-treated using 3% HCl. Enzymatic saccharification using thermostable cellulases also validated the results where the highest saccharification yield i.e. 39.5% was observed for the sample pre-treated using same. Maximum enzymatic hydrolysis of 52.7% was obtained for 0.40 g of the pre-treated halophyte Atriplex crassifolia where Endo-1,4-β-glucanase (300U), Exo-1,4-β-glucanase (400U) and β-1,4-glucosidase (1000U) were simultaneously added and incubated for 6 h at 75°C. The reducing sugar slurry obtained after optimization of saccharification was utilized as glucose in submerged fermentation for bioethanol production. The fermentation medium was inoculated with Saccharomyces cerevisiae, incubated at 30°C and 180 rpm for 96 h. Ethanol production was estimated using potassium dichromate method. Maximum production of bioethanol i.e. 16.33% was noted at 72 h. It can be concluded from the study that Atriplex crassifolia owing to its high cellulosic content after pre-treatment using dilute acid method, yields substantial amount of reducing sugars and high saccharification rates when subjected to enzymatic hydrolysis using thermostable cellulases, under optimized reaction conditions. Hence, the halophyte Atriplex crassifolia is a beneficial substrate that can be utilized to extract fermentable saccharides for bioethanol production.

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Section: Resultssupporting

confidence: 91%

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases

Nawaz

Qadoos²,

Haq³

et al. 2023

Front. Bioeng. Biotechnol.

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“…1. Other reports 42–46 are present on the cloning and expression of xylosidase gene in E. coli from various sources but till now no report is present on the cloning and expression of xylosidase gene from C. clariflavum .…”

Section: Discussionmentioning

confidence: 99%

“…43,89,96,97 Effect of recombinant xylosidase on the hydrolysis of wheat straw was observed in similar pattern like higher percentage hydrolysis (8.91%) again pretreated wheat straw biomass. Many reports are present on the plant biomass hydrolysis by xylosidase enzyme from various microorganisms 20,45,84,98–100 but not any report on hydrolysis of recombinant xylosidase enzyme from C. clariflavum yet present.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry

et al. 2022

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“…pET expression vectors contain the T7 promoter system and are frequently utilized for the heterologous expression of proteins. 20–24…”

Section: Introductionmentioning

confidence: 99%

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

et al. 2022

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Expression of thermostable β-xylosidase in Escherichia coli for use in saccharification of plant biomass

Cited by 10 publications

References 25 publications

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases

Effect of pretreatment strategies on halophyte Atriplex crassifolia to improve saccharification using thermostable cellulases

Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry

Heterologous expression, molecular studies and biochemical characterization of a novel alkaline esterase gene fromBacillus thuringiensisfor detergent industry

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