Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry

Abstract

The present study describes the cloning, expression, purification and characterization of a xylosidase gene from Clostridium clariflavum into E. coli BL21 (DE3) using the expression vector pET-21a(+) for utilization in biofuel production.

Improved secretory expression and characterization of thermostable xylanase and β-xylosidase from Pseudothermotoga thermarum and their application in synergistic degradation of lignocellulose

“…The optimal pH and temperature for purified XynB were 6.5 and 95°C ( Figures 4A, B ), which possessed the general properties of previously reported β -xylosidase that were more active in slightly acidic solution (pH 4.5-6.5) ( Wang and Arioka, 2021 ; Mercado-Flores et al, 2022 ; Zafar et al, 2022 ). The purified XynB was stable over a wide range of pH (5.5-8.5), retaining >80% of its initial activity after 2 h of incubation at 80°C ( Figure 4C ).…”

Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into E. coliBL21(DE3) for detergent formulation

“…Amplified lipase gene of B. thuringensis was successfully expressed by using pET-21a(+) in E. coli BL21(DE3). Previously many genes have been heterologously expressed by using pET expression vectors (Aftab et al, 2012(Aftab et al, , 2017Iqbal et al, 2015;Zafar et al, 2021;Zafar et al, 2016aZafar et al, , 2022a. To evaluate the gene expression and molecular mass determination, SDS-PAGE was used (Figure 2).…”

“…Due to the insertion of the RNA polymerase from phage T7 under the lacUV5 promoter in this bacterium's genome, this strain is one of the prominent strain of the T7 expression system. pET vector is an expression vector used for the expression of recombinant protein in E. coli (Aftab et al, 2012;Zafar et al, 2016bZafar et al, , 2022a.…”

Enhancement of Pulp Brightness and Yield in ECF Bleaching Using Xylanase Treatment: A Comparative Study on Different Wood Species

“…Seeking a natural resources candidate for the biofuel industry, a recombinant enzyme of xylanase and Xylosidase is used to convert hemicelluloses into xylose sugar. This research uses Xylosidase to effectively hydrolyze xylan into monomeric sugars, acting upon non-reducing ends of the hemicellulose fragment generated by xylanase enzyme action [7].…”