Expression of the Netrin-1 receptor, deleted in colorectal cancer (DCC), is largely confined to projecting neurons in the developing forebrain

Shu, Tianzhi; Valentino, Kimberly M.; Seaman, Clare; Cooper, Helen; Richards, Linda J.

doi:10.1002/(sici)1096-9861(20000110)416:2<201::aid-cne6>3.0.co;2-z

Cited by 88 publications

(76 citation statements)

References 52 publications

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“…Sections were incubated in either rabbit anti-GFAP (1:30,000; Z0334, Dako, Glostrup, Denmark); rabbit anti-neurofilament M C-terminal (1:75,000; AB1987, Chemicon, Temecula, CA); rat anti-L1 (1:5000; MAB5272, Chemicon); rabbit anticalbindin (1:10000; D-28K, Swant, Bellinzona, Switzerland); mouse anti-GAD65 (1:200 Affinity Research Products, Exeter, UK); or rabbit antiRobo1 or -Robo2 (1:10,000 and 1:5,000, respectively; antibodies prepared by Dr Murakami) overnight. Sections were washed in PBS and incubated in biotinylated goat anti-rabbit (1:500; Vector Laboratories) or biotinylated donkey anti-rat (1:500; Jackson ImmunoResearch Laboratory) for 2 hours, then incubated in avidin-biotin solution (1:500; Vector Laboratories) and processed as previously described (Shu et al, 2000).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain

et al. 2006

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The Slit genes encode secreted ligands that regulate axon branching, commissural axon pathfinding and neuronal migration. The principal identified receptor for Slit is Robo (Roundabout in Drosophila). To investigate Slit signalling in forebrain development, we generated Robo1 knockout mice by targeted deletion of exon 5 of the Robo1 gene. Homozygote knockout mice died at birth, but prenatally displayed major defects in axon pathfinding and cortical interneuron migration. Axon pathfinding defects included dysgenesis of the corpus callosum and hippocampal commissure, and abnormalities in corticothalamic and thalamocortical targeting. Slit2 and Slit1/2 double mutants display malformations in callosal development, and in corticothalamic and thalamocortical targeting, as well as optic tract defects. In these animals, corticothalamic axons form large fasciculated bundles that aberrantly cross the midline at the level of the hippocampal and anterior commissures, and more caudally at the medial preoptic area. Such phenotypes of corticothalamic targeting were not observed in Robo1 knockout mice but, instead, both corticothalamic and thalamocortical axons aberrantly arrived at their respective targets at least 1 day earlier than controls. By contrast, in Slit mutants, fewer thalamic axons actually arrive in the cortex during development. Finally, significantly more interneurons (up to twice as many at E12.5 and E15.5) migrated into the cortex of Robo1 knockout mice, particularly in both rostral and parietal regions, but not caudal cortex. These results indicate that Robo1 mutants have distinct phenotypes, some of which are different from those described in Slit mutants, suggesting that additional ligands, receptors or receptor partners are likely to be involved in Slit/Robo signalling.

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Section: Immunohistochemistrymentioning

confidence: 99%

Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain

et al. 2006

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“…First, because olfactory bulb neurons transiently express Dcc on their axons (Shu et al, 2000;Inaki et al, 2004), it is possible that olfactory bulb axons are directly guided by netrin 1, so that the axons in the mutant embryos are misdirected as a direct consequence of the loss of netrin 1-Dcc signaling. However, netrin 1 does not exert any apparent effects on olfactory bulb axons in culture (Li et al, 1999;de Castro et al, 1999), and therefore we could not address this possibility any further.…”

Section: Research Articlementioning

confidence: 99%

Netrin 1 regulates ventral tangential migration of guidepost neurons in the lateral olfactory tract

2006

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MiceTime-pregnant ICR wild-type mice were purchased from SLC (Shizuoka, Japan). Mice expressing green fluorescent protein (GFP; green mice) were provided by Okabe et al. (Okabe et al., 1997). Xt J /Xt J mutant embryos, which have deletions in the Gli3 gene, were generated as described previously (Tomioka et al., 2000). When the Xt J mutation was introduced into green mice, green mice were crossed with Xt J /+ mice and the resulting Xt J /+ mice expressing GFP were intercrossed to obtain Xt J /Xt J green mouse embryos. Heterozygous netrin 1 mutant mice (Serafini et al., 1996) and Dcc mutant mice (Fazeli et al., 1997) were crossed to obtain homozygous embryos. The day on which a vaginal plug was found was designated embryonic day 0.5 (E0.5). All experimental protocols were approved by the Animal Committee of National Institute of Genetics. Organotypic culture of telencephalon stripsEach telencephalic hemisphere of the E10.5 mouse embryo was dissected along the dorsoventral axis into a strip ~1.5 mm wide, including the neocortex, the presumptive LOT area and the lateral and medial ganglionic eminences (GE) (see Fig. 1A). In the strip, the presumptive LOT area was located around the middle along the dorsoventral axis. In a standard culture, the strip was labeled by injection of dextran tetramethylrhodamine In the developing nervous system, functional neural networks are constructed with intricate coordination of neuronal migrations and axonal projections. We have previously reported a ventral tangential migration of a special type of cortical neurons, lot cells, in the mouse embryo. These neurons originate from the ventricular zone of the entire neocortex, tangentially migrate in the surface layer of the neocortex into the ventral direction, align in the future pathway of the lateral olfactory tract (LOT) and eventually guide the projection of LOT axons. In this study, we developed an organotypic culture system to investigate the regulation of this cell migration in the developing telencephalon. Our data show that the neocortex contains the signals that direct lot cells ventrally, that the ganglionic eminence excludes lot cells by repelling the migration and that lot cells are attracted to netrin 1, an axon guidance factor. Furthermore, we demonstrate that mutations in the genes encoding netrin 1 and its functional receptor Dcc lead to inappropriate distribution of lot cells and subsequent partial disruption of LOT projection. These results suggest that netrin 1 regulates the migration of lot cells and LOT projections, possibly by ensuring the correct distribution of these guidepost neurons. Molecular Probes, Eugene, OR) or 1,1-dioctadecyl-3,3,3Ј3Ј-tetrametylindocarbacyanine perchlorate (DiI; Molecular Probes) into a small area of the dorsal neocortex, unfolded on a collagen-coated membrane filter (Transwell-COL inserts #3492, Corning, Acton, MA) and cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 (Sigma-Aldrich, St Louis, MO) containing 10% fetal bovine serum (Cansera, Rexdale, ONT, Canada) and 5% hor...

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“…76 Functional receptors for secreted molecules of the Netrin family, such as DCC or Unc5H3 are strongly expressed by developing mitral cells of the OB. 77,78 However, neither secreted Netrin-1 nor a secreted form of the Netrin-G1 (anchored to plasmalemma membrane) exert any effect on OB axonal outgrowth. 17,72,79 It has been suggested that a stereotypic map of mitral cell termination in the olfactory cortex might be established by odor receptors providing an input to those mitral cells.…”

Section: Cell Death and Differentiationmentioning

confidence: 99%

The olfactory bulb as an independent developmental domain

López‐Mascaraque

Castro

2002

Cell Death Differ

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The olfactory system is a good model to study the mechanisms underlying guidance of growing axons to their appropriate targets. The formation of the olfactory bulb involves differentiation of several populations of cells and the initiation of the central projections, all under the temporal and spatial patterns of gene expression. Moreover, the nature of interactions between the olfactory epithelium, olfactory bulb and olfactory cortex at early developmental stages is currently of great interest. To explore these questions more fully, the present review aims to correlate recent data from different developmental studies, to gain insight into the mechanisms involved in the specification and development of the olfactory system. From our studies in the pax6 mutant mice (Sey Neu /Sey Neu ), it was concluded that the initial establishment of the olfactory bulb central projections is able to proceed independently of the olfactory sensory axons from the olfactory epithelium. The challenge that now remains is to consider the validity of the olfactory bulb as an independent development domain. In the course of evaluating these ideas, we will review the orchestra of molecular cues involved in the formation of the projection from the OB to the olfactory cortex.

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Expression of the Netrin-1 receptor, deleted in colorectal cancer (DCC), is largely confined to projecting neurons in the developing forebrain

Cited by 88 publications

References 52 publications

Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain

Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain

Netrin 1 regulates ventral tangential migration of guidepost neurons in the lateral olfactory tract

The olfactory bulb as an independent developmental domain

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