Expression of the mRNAs encoding the limbic system‐associated membrane protein (LAMP): I. Adult rat brain

Pimenta, Aurea F.; Reinoso, Blesilda S.; Levitt, Pat

doi:10.1002/(sici)1096-9861(19961111)375:2<274::aid-cne7>3.0.co;2-#

Cited by 49 publications

(20 citation statements)

References 75 publications

(85 reference statements)

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“…At least three families of growth-regulating molecules have been identified as candidates for the guidance signals in the developing cortex and thalamus. First, the Ig superfamily members such as the limbic-associated membrane protein (LAMP) and neurotrimmin are found in distinct limbic and primary sensory and motor regions, respectively (19,36,37), and have been shown to promote neurite outgrowth in a homophilic fashion in vitro (19,38), indicating that positive guidance cues are essential for appropriate targeting of axon populations in the cortex. This notion is supported by in vivo transplant studies in which LAMP expression correlates precisely with the ability to form limbic thalamocortical (34) and cortico-cortical (39) connections of each graft with the host brain.…”

Section: Discussionmentioning

confidence: 99%

Regulation of thalamic neurite outgrowth by the Eph ligand ephrin-A5: Implications in the development of thalamocortical projections

Gao

Yue

Zhang

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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The cerebral cortex is parcellated into different functional domains that receive distinct inputs from other cortical and subcortical regions. The molecular mechanisms underlying the specificity of connections of cortical afferents remain unclear. We report here that the Eph family tyrosine kinase receptor EphA5 and the ligand ephrin-A5 may play a key role in the exclusion of the limbic thalamic afferents from the sensorimotor cortex by mediating repulsive interactions. In situ hybridization shows that the EphA5 transcript is expressed at high levels in both cortical and subcortical limbic regions, including the frontal cortex, the subiculum, and the medial thalamic nuclei. In contrast, ephrin-A5 is transcribed abundantly in the sensorimotor cortex. Consistent with the complementary expression, the ligand inhibited dramatically the growth of neurites from neurons isolated from the medial thalamus but was permissive for the growth of neurites from lateral thalamic neurons, which is primarily nonlimbic. Similarly, the growth of neurites from Eph-A5-expressing neurons isolated from the subiculum was inhibited by ephrin-A5. Our studies suggest that the Eph family ligand ephrin-A5 serves as a general inhibitor of axonal growth from limbic neurons, which may serve to prevent innervation of inappropriate primary sensorimotor regions, thus contributing to the generation of specificity of thalamic cortical afferents.The cerebral cortex is parcellated into multiple domains that subserve different functions (1-3). The medial cortical areas, which include prefrontal, cingulate, and retrosplenial cortices, are components of limbic circuits, in contrast to nonlimbic sensory and motor cortices (3, 4). The limbic cortices receive projections from nuclei in the medial and anterior thalamus and from other limbic areas, including the hippocampal formation (5). In contrast, the sensorimotor cortex is innervated specifically by primary sensory and motor thalamic nuclei located in the ventrolateral and posterior thalamus (5). This topographic arrangement of mature functional pathways in the adult brain is paralleled by an early specificity of thalamocortical projections during development (2, 6, 7). This specificity suggests the presence of guidance mechanisms that facilitate the formation of distinct projection patterns.In the classic model of topographic map formation, Sperry (8, 9) proposed that molecular tags form gradients in projecting and target fields and interact to guide axons to appropriate regions. Such guidance cues have been identified recently and have been shown to be receptors and ligands of the Eph family of tyrosine kinases (10-13). In the retinotectal topographic map, the Eph family receptor EphA3 is expressed by retinal ganglion neurons in a nasal (low) to temporal (high) gradient whereas two ligands, ephrin-A2 and -A5, are distributed in a complementary anterior (low) to posterior (high) gradient in the tectum (10, 14). In the hippocamposeptal projection, the Eph family receptor EphA5 is expressed in a l...

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Section: Discussionmentioning

confidence: 99%

Regulation of thalamic neurite outgrowth by the Eph ligand ephrin-A5: Implications in the development of thalamocortical projections

Gao

Yue

Zhang

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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142

111

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“…In that study, Kilon immunoreactivity was detected on the dendrites and somata of pyramidal neurons in the hippocampus CA1 region and in the cerebral cortex, an expression pattern believed to be similar to that of LAMP Levitt, 1989, Pimenta et al, 1996;Reinoso et al, 1996). We used in situ hybridization to analyze the distribution of Kilon in the developing and adult rat brain in comparison to LAMP and found that Kilon mRNA expression shows clear differences from that of LAMP during development, leading to a pattern in the adult hippocampus in which Kilon mRNA synthesis is restricted almost exclusively to the adult DG.…”

mentioning

confidence: 85%

“…U31554) were used. As an additional control for the specificity of the LAMP signal, we used the antisense oligonucleotide designed by Reinoso et al (1996) (5Ј-GTG GTA CGG CCA GAC TGA TGG ATC CGT TGA TTC CTC TCA CCG ACC CGG GTC TG-3Ј) complementary to bases 973-1025 of the rat LAMP cDNA (GenBank accession no. U31554).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

IG-molecule Kilon shows differential expression pattern from LAMP in the developing and adult rat hippocampus

et al. 2000

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“…The limbic system-associated membrane protein (LAMP), a cell adhesion molecule (CAM) of the IgLON family expressed in cortical and subcortical limbic-associated regions of the developing and adult brain [5,6,20,29,39,44,54] is one such molecule. The protein exhibits 99% homology between rodent and human [38] and there is a close correlation between Lsamp mRNA and protein distribution patterns in rat [29,39,44,54], monkey [5,6], and human [42,43].…”

Section: Introductionmentioning

confidence: 99%

“…The protein exhibits 99% homology between rodent and human [38] and there is a close correlation between Lsamp mRNA and protein distribution patterns in rat [29,39,44,54], monkey [5,6], and human [42,43]. Experimental manipulations of LAMP in vitro result in altered axon targeting and neurite growth [13,24,31,41,56].…”

Section: Introductionmentioning

confidence: 99%

Genetic Deletion of Lsamp Causes Exaggerated Behavioral Activation in Novel Environments

Catania

Pimenta

Levitt

2007

Behavioural Brain Research

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The limbic system-associated membrane protein (LAMP) is a GPI-anchored cell adhesion molecule expressed heavily in limbic and limbic-associated regions of the developing and adult brain. Experimental studies show that LAMP promotes the growth of limbic neurons and guides the projections of limbic fibers. In order to examine the functional consequences of disrupting limbic circuit assembly, we generated a mouse line in which the Lsamp gene encoding LAMP was deleted. Basic neuroanatomical organization and sensory and motor development are normal in Lsamp −/− mice. The most profound change in behavior in both male and female Lsamp −/− mice is a heightened reactivity to novelty exhibited in several behavioral tests. Lsamp −/− mice display hyperactivity in a novel arena and both sexes habituate to the same activity levels as their wildtype littermates, but at different rates. In the elevated plus maze, Lsamp −/− mice exhibit increased total arm entries, with a bias towards the open arms; they spend more time in the open arms and have a substantial increase in the amount of risk assessment in unprotected areas of the maze. In the y-maze, Lsamp −/− mice exhibit characteristic hyperactivity and a decreased level of spontaneous alternation during the period when their novelty-induced hyperactivity is at its peak. We hypothesize that Lsamp −/− mice may not simply exhibit a decrease in anxiety, but may have a heightened, and possibly maladaptive, response to novel environmental stressors. Genetic deletion of Lsamp may thus cause circumscribed changes in the fine connectivity of specific circuits that underlie these behaviors.

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Expression of the mRNAs encoding the limbic system‐associated membrane protein (LAMP): I. Adult rat brain

Cited by 49 publications

References 75 publications

Regulation of thalamic neurite outgrowth by the Eph ligand ephrin-A5: Implications in the development of thalamocortical projections

Regulation of thalamic neurite outgrowth by the Eph ligand ephrin-A5: Implications in the development of thalamocortical projections

IG-molecule Kilon shows differential expression pattern from LAMP in the developing and adult rat hippocampus

Genetic Deletion of Lsamp Causes Exaggerated Behavioral Activation in Novel Environments

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