Expression of the Mel
            <sub>1a</sub>
            ‐melatonin receptor mRNA in T and B subsets of lymphocytes from rat thymus and spleen

Pozo, David; Delgado, Mario; Fernández-Santos, José M.; Calvo, Juan R.; Gomariz, Rosa P.; Martín-Lacave, Inés; Ortíz, Genaro Gabriel; Guerrero, Juan M.

doi:10.1096/fasebj.11.6.9194527

Cited by 138 publications

(102 citation statements)

References 32 publications

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“…The results showed that positive cells located in germinal epithelium, extraovarian rete, follicular cells of the primordial follicle, primary follicle and oocytes in every follicle grade, and the staining intensity was strong. The presence of melatonin receptors in the ovary, mammary gland, testis, epididymis indicate that the direct melatonin actions on different levels of the reproductive system (Pang et al 1998;Pozo et al 1997). It indicates that there should be a part of melatonin effect on the ovary of ostrich chicks directly, besides the indirect effect through the hypothalamic-pituitary-gonadal axis.…”

Section: Discussionmentioning

confidence: 99%

“…Ovary tissue samples from ostrich chicks were centrifuged 10 min at 3000 g in icecold lysis buffer [17 mM Tris-HCl pH 7.3 containing 0.144 M NH4Cl (Sigma)] to eliminate lymphocytes that express melatonin receptors (Pozo et al 1997). Tissues were homogenized using a Potter-Elvehjem homogenizer (20 strokes, on ice).…”

Section: Protein Isolation and Western Blot Analysismentioning

confidence: 99%

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Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

Wang

Peng

et al. 2008

Cytotechnology

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Healthy 90-day-old ostrich chicks were used in the present study. The ultrastructure and melatonin 1a receptor (MT1) distribution in the ovaries of ostrich chicks was observed by transmission electron microscope and light microscope. The results showed that the ostrich chick ovary contained primordial follicles, primary follicles and secondary follicles, but no mature follicles. There are some unique ultrastructural characteristics observed in the secondary follicle, such as the cortical granule, which was located in cytoplasm beside the nucleus and appeared first in the oocyte. The zona radiata appeared in the secondary follicle, and there was an obvious vitelline membrane. There were intraovarian rete, connecting rete, and extraovarian rete in the ovaries of ostrich chicks. This is the first study that provides immunohistochemical evidence for the localization of the melatonin MT1 in the ostrich chick ovary. The germinal epithelium, follicular cell layer of every grade of follicle, cytoplasm of the oocyte and interstitial cells all expressed MT1. The expression of positive immunoreactivity materials was the strongest in the follicular cell layer of the primordial follicle and germinal epithelium, was weaker in the follicular cell layer of the primary follicle and secondary follicle, and was weakest in the oocytes of all grades of follicle. In addition, the extraovarian rete displayed strong positive expression of MT1, while there was no positive expression in the intraovarian rete or connecting rete. The positive expression of MT1 immunoreactivity in the ovary was very strong, implying that the ovary is an important organ for synthesizing MT1.

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Section: Discussionmentioning

confidence: 99%

Section: Protein Isolation and Western Blot Analysismentioning

confidence: 99%

Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

Wang

Peng

et al. 2008

Cytotechnology

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“…The evidence so far suggests that melatonin can influence immune cells through nuclear and membrane melatonin receptors. These receptors are found on macrophages, B cells, and T cells (Garcıá-Pergañeda et al 1999;Pozo et al 1997). Melatonin can modulate proliferation and cytokine secretion via these receptors on immune cells (García-Mauriño et al 2000;GarciaMaurino et al 1997).…”

Section: Role Of Melatonin In Immune Responses To Radiationmentioning

confidence: 99%

The melatonin immunomodulatory actions in radiotherapy

et al. 2017

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Radiotherapy has a key role in cancer treatment in more than half of patients with cancer. The management of severe side effects of this treatment modality is a limiting factor to appropriate treatment. Immune system responses play a pivotal role in many of the early and late side effects of radiation. Moreover, immune cells have a significant role in tumor response to radiotherapy, such as angiogenesis and tumor growth. Melatonin as a potent antioxidant has shown appropriate immune regulatory properties that may ameliorate toxicity induced by radiation in various organs. These effects are mediated through various modulatory effects of melatonin in different levels of tissue reaction to ionizing radiation. The effects on the DNA repair system, antioxidant enzymes, immune cells, cytokines secretion, transcription factors, and protein kinases are most important. Moreover, anti-cancer properties of melatonin may increase the therapeutic ratio of radiotherapy. Clinical applications of this agent for the management of malignancies such as breast cancer have shown promising results. It seems anti-proliferative, anti-angiogenesis, and stimulation or suppression of some immune cell responses are the main anti-tumor effects of melatonin that may help to improve response of the tumor to radiotherapy. In this review, the effects of melatonin on the modulation of immune responses in both normal and tumor tissues will be discussed.

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“…T cell apoptosis may also be blocked or partially blocked by zinc (359). In this context it is interesting to note the claim that the beneficial effects of melatonin supplementation or pineal grafting are associated with increased plasma zinc levels in old mice in the absence of exogenous zinc supplementation (532) (although melatonin may have direct effects on lymphocytes, which express melatonin receptors (533), ligation of which results in signal transduction and diacylglycerol production (534)). It is argued by Fabris et al that the common pathway of several life-extending endocrinological manipulations is in fact via zinc bioavailability (535).…”

Section: Vitamins and Minerals; Anti-oxidantsmentioning

confidence: 99%

T cells and aging update February 1999

Pawelec¹

1999

Front Biosci

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T cell immunosenescence 218effects of dysregulated immune responses in the elderly by supplementation or other approaches may result in an enhancement of their quality of life, and significant reductions in the cost of medical care in old age. FACTORS CONTRIBUTING TO IMMUNO-SENESCENCE HematopoiesisDysregulated hematopoiesis is seen in elderly individuals, raising the possibility that this could contribute to altered immune function in the aged. Hematopoiesis in man may be compromised because of a severely reduced capacity to produce colony stimulating factors (44) and increased production of pro-inflammatory factors such as IL 6 (45), because lower numbers of progenitor cells are present in the BM (46) and because of an age-related decline in proliferative potential of putative haematopoietic stem cells (HSC) (47). In mice, the repopulating potential of murine fetal liver-derived HSC is higher than that of adult BM-derived stem cells (48), suggesting possible aging of the HSC themselves. Normal cells with shorter telomeres possess less remaining replicative capacity than those with longer telomeres (see section 5.2). Accordingly, HSC from adult BM were found to have shorter telomeres than fetal liver-derived or umbilical cord-derived stem cells, consistent with aging of HSC (49). Telomere lengths decreased on culture despite low level expression of telomerase in these cells (50,51), although the rate of basepair loss per population doubling of cells in culture was lower during the first two weeks, when telomerase was upregulated, than in the next two, when it was downregulated (52). The telomerase expressed is therefore functionally active but may not be able to completely maintain telomere length in aging HSC cells. True embryonic stem cells (ESC), on the other hand, which may really be immortal, do retain high levels of telomerase activity (53). On the other hand, ESC from telomerase-knockout mice show gradual telomere shortening over many population doublings (PD) resulting in slowing and eventual cessation of growth (54). Certain animals which do not downregulate telomerase manifest a permanent growth phase and little or no senescence (55,56).

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Expression of the Mel _1a ‐melatonin receptor mRNA in T and B subsets of lymphocytes from rat thymus and spleen

Cited by 138 publications

References 32 publications

Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

The melatonin immunomodulatory actions in radiotherapy

T cells and aging update February 1999

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Expression of the Mel 1a ‐melatonin receptor mRNA in T and B subsets of lymphocytes from rat thymus and spleen

Cited by 138 publications

References 32 publications

Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

Ultrastructure and melatonin 1a receptor distribution in the ovaries of African ostrich chicks

The melatonin immunomodulatory actions in radiotherapy

T cells and aging update February 1999

Contact Info

Product

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Expression of the Mel _1a ‐melatonin receptor mRNA in T and B subsets of lymphocytes from rat thymus and spleen