Expression of the entomotoxic Cocculus hirsutus trypsin inhibitor (ChTI) gene in transgenic chickpea enhances its underlying resistance against the infestation of Helicoverpa armigera and Spodoptera litura

Pandey, Ankesh; Yadav, Reena; Kumar, Sanoj; Kumar, Anil; Shukla, Priya; Yadav, Ankita; Sanyal, Indraneel

doi:10.1007/s11240-021-02041-2

Cited by 8 publications

(8 citation statements)

References 39 publications

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“…Recently a Kunitz‐type TI from Cocculus hirsutus (ChTI) was expressed in chickpea. In this report, 60–80% mortality along with weight loss (30–60%) for S. litura and Helicoverpa armigera was found 73 . Plant lectins, chitinases, lipoxygenases and cholesterol oxidases are also used to develop insect‐resistant transgenic plants.…”

Section: Discussionmentioning

confidence: 83%

“…The 12 plant PI families are illustrated on the basis of structural, functional and amino acid residues in the reactive site of target proteases. ChTI ( Cocculus hirsutus trypsin inhibitor, a typical Kunitz type PPI) has been reported to inhibit trypsin (T in pink circles) in Helicoverpa armigera causing mortality and weight loss by feeding on the chickpea plant harbouring ChTI gene 73 …”

Section: Ppis: Rapid Action Defence Against Insectsmentioning

confidence: 99%

“…The cowpea TI gene ( CpTI ) was first PI (Bowman–Birk type) to be overexpressed in tobacco and later was inserted in other plant genomes such as sweet potato, potato cotton, rice, cabbage, strawberry and pigeon pea to improve insect resistance (Table 3). 66,73–93 …”

Section: Plant Serine Pis: Biotechnological Applicationsmentioning

confidence: 99%

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Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

Pandey

Yadav

Sanyal

2021

Pest Management Science

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In the arsenal of plant defense, protease inhibitors (PIs) are well-designed defensive products to counter field pests. PIs are produced in plant tissues by means of 'stable defense metabolite' and triggered on demand as the perception of the signal and well established as a part of plant active defense. PIs have been utilized for approximately four decades, initially as a gene-alone approach that was later replaced by multiple gene pyramiding/gene stacking due to insect adaptability towards the PI alone. By considering the adaptive responses of the pest to the single insecticidal gene, the concept of gene pyramiding gained continuous appreciation for the development of transgenic crops to deal with co-evolving pests. Gene pyramiding approaches are executed to bypass the insectʼs adaptive responses against PIs. Stacking PIs with additional insecticidal proteins, plastid engineering, recombinant proteinase inhibitors, RNAi-based methods and CRISPR/Cas9-mediated genome editing are the advanced tools and methods for next-generation pest management. Undoubtedly, the domain associated with the mechanism of PIs in the course of plant-pest interactions will occupy a central role for the advancement of more efficient and sustainable pest control strategies.

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Section: Discussionmentioning

confidence: 83%

Section: Ppis: Rapid Action Defence Against Insectsmentioning

confidence: 99%

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Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

Pandey

Yadav

Sanyal

2021

Pest Management Science

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“…The crop is also vulnerable to biotic stresses, which further reduce the yield and include collar rot, dry root rot, Ascochyta blight, Helicoverpa , Fusarium wilt, Botrytis grey mold, and seasonal weeds. The main fungi that damage chickpea plants are Ascochyta rabiei ; causes Ascochyta blight and Fusarium oxysporum ; which causes wilting, which is the most dangerous disease (producing 100% deaths in some cases) [ 11 , 69 , 70 ]. Blight is characterized by brown blotches on stems, seeds, pods, and leaves.…”

Section: Abiotic and Biotic Constraints To Chickpea Productionmentioning

confidence: 99%

Chickpea (Cicer arietinum L.) Biology and Biotechnology: From Domestication to Biofortification and Biopharming

Koul

Sharma

Sehgal

et al. 2022

Plants

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Chickpea (Cicer arietinum L.), the world’s second most consumed legume crop, is cultivated in more than 50 countries around the world. It is a boon for diabetics and is an excellent source of important nutrients such as vitamins A, C, E, K, B1–B3, B5, B6, B9 and minerals (Fe, Zn, Mg and Ca) which all have beneficial effects on human health. By 2050, the world population can cross 9 billion, and in order to feed the teaming millions, chickpea production should also be increased, as it is a healthy alternative to wheat flour and a boon for diabetics. Moreover, it is an important legume that is crucial for food, nutrition, and health security and the livelihood of the small-scale farmers with poor resources, in developing countries. Although marvelous improvement has been made in the development of biotic and abiotic stress-resistant varieties, still there are many lacunae, and to fulfill that, the incorporation of genomic technologies in chickpea breeding (genomics-assisted breeding, high-throughput and precise-phenotyping and implementation of novel breeding strategies) will facilitate the researchers in developing high yielding, climate resilient, water use efficient, salt-tolerant, insect/pathogen resistant varieties, acceptable to farmers, consumers, and industries. This review focuses on the origin and distribution, nutritional profile, genomic studies, and recent updates on crop improvement strategies for combating abiotic and biotic stresses in chickpea.

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“…Developing biotic and abiotic stress-resistant genotypes using conventional breeding is a timeconsuming and challenging task. The biotechnological methods offer prospects for improving chickpea and transgenic chickpea with novel traits, especially resistance against different pathogens [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Expression of radish defensin (RsAFP2) gene in chickpea (Cicer arietinum L.) confers resistance to Fusarium wilt disease

et al. 2022

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Chickpea (Cicer arietinum L.) is a major nutritional source cultivated worldwide. Chickpea is vulnerable to several abiotic and biotic stresses, including different types of soil-borne pathogens like Fusarium oxysporum f. sp. ciceri, which causes root rot disease and severely affects productivity. The putative transgenic plants were obtained with the Radish defensin (Rs-AFP2) gene through Agrobacterium tumefaciens mediated transformation using the embryo axis explants. Transgenes were con rmed in 18 putative transgenic plants with PCR-speci c primers for nptII and Rs-AFP2 genes. Twelve transgenic plants were established successfully under greenhouse conditions. The T0 plants were allowed for selfpollination and obtained T1 seeds. The T1 plants were selected for Fusarium wilt assay using Fusarium oxysporum f. sp. ciceri. The T1 plants showed different resistance levels, from moderate to high levels. The control plants (wild-type) exhibited severe wilt symptoms after Fusarium infection.

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Expression of the entomotoxic Cocculus hirsutus trypsin inhibitor (ChTI) gene in transgenic chickpea enhances its underlying resistance against the infestation of Helicoverpa armigera and Spodoptera litura

Cited by 8 publications

References 39 publications

Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

Chickpea (Cicer arietinum L.) Biology and Biotechnology: From Domestication to Biofortification and Biopharming

Expression of radish defensin (RsAFP2) gene in chickpea (Cicer arietinum L.) confers resistance to Fusarium wilt disease

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