Expression of the alpha toxin of Clostridium perfringens in Lactobacillus casei genome and evaluation of its immune effects in mice

Song, Baifen; Cui, Hongyu; Ju, Longzhu; Song, Li; Tang, Lijie; Li, Yijing

doi:10.1016/j.micpath.2018.03.010

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…Because lactobacilli are safe and may have immune-stimulating adjuvant effects [3][4][5][6][7][8] , they are promising delivery vectors for antigens and other medical molecules. Studies with animal models have repeatedly demonstrated the potential of antigen producing lactobacilli to induce specific immune responses [9][10][11][12][13][14][15][16] and one such Lactobacillus has even reached clinical tests 17 . Ideally, the antigens should be sufficiently protected from proteolytic digestion and other damage in the harsh environment of the gastro-intestinal tract, while at the same time being sufficiently exposed to provoke favorable immune responses at mucosal surfaces.…”

mentioning

confidence: 99%

Anchoring of heterologous proteins in multiple Lactobacillus species using anchors derived from Lactobacillus plantarum

Mathiesen

Øverland

Kuczkowska

et al. 2020

Sci Rep

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Members of the genus Lactobacillus have a long history in food applications and are considered as promising and safe hosts for delivery of medically interesting proteins. We have assessed multiple surface anchors derived from Lactobacillus plantarum for protein surface display in multiple Lactobacillus species, using a Mycobacterium tuberculosis hybrid antigen as test protein. the anchors tested were a lipoprotein anchor and two cell wall anchors, one non-covalent (LysM domain) and one covalent (sortase-based anchoring using the LPXTG motif). Thus, three different expression vectors for surface-anchoring were tested in eight Lactobacillus species. When using the LpXtG and LysM cell wall anchors, surface display, as assessed by flow cytometry and fluorescence microscopy, was observed in all species except Lactobacillus acidophilus. Use of the cell membrane anchor revealed more variation in the apparent degree of surface-exposure among the various lactobacilli. Overproduction of the secreted and anchored antigen impaired bacterial growth rate to extents that varied among the lactobacilli and were dependent on the type of anchor. overall, these results show that surface anchors derived from L. plantarum are promising candidates for efficient anchoring of medically interesting proteins in other food grade Lactobacillus species.

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confidence: 99%

Anchoring of heterologous proteins in multiple Lactobacillus species using anchors derived from Lactobacillus plantarum

Mathiesen

Øverland

Kuczkowska

et al. 2020

Sci Rep

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“…Reportedly, NK cell depletion can improve hepatic injury and survival (Kawabata et al, 2008;Qu et al, 2015). In addition, studies have shown that iron overload is closely related to L. casei supplementation (Song et al, 2018). We have previously reported that L. casei can regulate the proportion of T lymphocyte subsets and NK cells, thus improving the immune function of rats with alcoholic liver injury or breast cancer (Zhengyan et al, 2017;Yiyun et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Aktas et al have reported that L. casei can alter the gut microbiota composition and modulate the host immune response (Aktas et al., 2016 ). Song et al have indicated that mice demonstrate humoral immunity and cellular immunity after L. casei supplementation (Song et al., 2018 ). We have previously reported that L. casei can regulate the proportion of T lymphocyte subsets and NK cells, thus improving the immune function of rats with alcoholic liver injury or breast cancer (Zhengyan et al., 2017 ; Yiyun et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus casei relieves liver injury by regulating immunity and suppression of the enterogenic endotoxin‐induced inflammatory response in rats cotreated with alcohol and iron

Han

Liu

et al. 2021

Food Science & Nutrition

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“…The probiotic bacteria Lactobacilli casei has been engineered to express a C. perfringens α-toxoid mimicking the α-toxin. Mice vaccinated with the probiotic developed anti-α toxin antibodies and survived challenge with a lethal dose α-toxin while the control animals rapidly succumbed to the effects of the toxin ( Alimolaei et al, 2018 ; Song et al, 2018 ; Gao et al, 2019 ). Oral administration of an ε -toxoid expressing L. casei strain or a β-toxoid expressing L. casei strain was also able to induce an effective antibody response to the toxin ( Alimolaei et al, 2016 ; Alimolaei et al, 2017 ).…”

Section: Synthetic Biology Approaches To Antimicrobial Drug Resistancementioning

confidence: 99%

Bioengineered Probiotics: Synthetic Biology Can Provide Live Cell Therapeutics for the Treatment of Foodborne Diseases

Cruz

Enekegho

Stuart

2022

Front. Bioeng. Biotechnol.

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The rising prevalence of antibiotic resistant microbial pathogens presents an ominous health and economic challenge to modern society. The discovery and large-scale development of antibiotic drugs in previous decades was transformational, providing cheap, effective treatment for what would previously have been a lethal infection. As microbial strains resistant to many or even all antibiotic drug treatments have evolved, there is an urgent need for new drugs or antimicrobial treatments to control these pathogens. The ability to sequence and mine the genomes of an increasing number of microbial strains from previously unexplored environments has the potential to identify new natural product antibiotic biosynthesis pathways. This coupled with the power of synthetic biology to generate new production chassis, biosensors and “weaponized” live cell therapeutics may provide new means to combat the rapidly evolving threat of drug resistant microbial pathogens. This review focuses on the application of synthetic biology to construct probiotic strains that have been endowed with functionalities allowing them to identify, compete with and in some cases kill microbial pathogens as well as stimulate host immunity. Weaponized probiotics may have the greatest potential for use against pathogens that infect the gastrointestinal tract: Vibrio cholerae, Staphylococcus aureus, Clostridium perfringens and Clostridioides difficile. The potential benefits of engineered probiotics are highlighted along with the challenges that must still be met before these intriguing and exciting new therapeutic tools can be widely deployed.

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Expression of the alpha toxin of Clostridium perfringens in Lactobacillus casei genome and evaluation of its immune effects in mice

Cited by 3 publications

References 40 publications

Anchoring of heterologous proteins in multiple Lactobacillus species using anchors derived from Lactobacillus plantarum

Anchoring of heterologous proteins in multiple Lactobacillus species using anchors derived from Lactobacillus plantarum

Lactobacillus casei relieves liver injury by regulating immunity and suppression of the enterogenic endotoxin‐induced inflammatory response in rats cotreated with alcohol and iron

Bioengineered Probiotics: Synthetic Biology Can Provide Live Cell Therapeutics for the Treatment of Foodborne Diseases

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