Expression of the alpha subunit of 7S nerve growth factor in the mouse submandibular gland

Isackson, Paul J.; Nisco, Steven J.; Bradshaw, Ralph A.

doi:10.1007/bf00966319

Cited by 8 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…added along with anti-a subunit IgG blocked precipitation of all four of these polypeptides (Figure 4c), demonstrating that immunoprecipitation was specific for all of them. The two higher molecular weight bands consistently appeared as a doublet with molecular weights -between 30,000 and 34,000, consistent with the size previously reported for the a subunit precursor (12,14). The lowest molecular weight band is the same size as the mature a subunit large chain (16,500).…”

Section: Selection Of a Subunit-related Cdna Clonessupporting

confidence: 88%

See 1 more Smart Citation

The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Fahnestock¹,

Brundage²,

Shooter³

1986

Nucl Acids Res

View full text Add to dashboard Cite

Mouse submaxillary gland contains many proteolytic enzymes, the most widely studied of which are the kallikreins. This gland also contains high levels of nerve growth factor (NGF), which is isolated as a complex of three subunits, alpha, beta, and gamma. We report here the cloning and sequence analysis of a novel kallikrein from mouse submaxillary gland. Antibodies directed against the alpha subunit precipitate the product of this clone, but do not precipitate the homologous gamma subunit. This new kallikrein is therefore closely related to alpha NGF, yet in contrast to the alpha subunit, its sequence suggests it has proteolytic activity.

show abstract

Section: Selection Of a Subunit-related Cdna Clonessupporting

confidence: 88%

“…(Figure 2). The size of the RNA (approximately 1000 bp) was sufficient to code for an oc subunit precursor, which has a molecular weight of approximately 32,000 (12,14).…”

Section: Selection Of a Subunit-related Cdna Clonesmentioning

confidence: 99%

The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Fahnestock¹,

Brundage²,

Shooter³

1986

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…It is thus intriguing to hypothesize a cleavage of the MSP precursor by NGF-␥ and epidermal growth factor-binding protein. These could be transported from the submandibular glands, where they are highly expressed (Drinkwater et al, 1987;Isackson et al, 1987), to the brainstem through the extensive connections that link together masticatory, facial, and lingual neuromuscular systems (Fay and Norgren, 1997).…”

Section: Discussionmentioning

confidence: 99%

Macrophage Stimulating Protein Is a Novel Neurotrophic Factor

Stella

Vercelli

Repici

et al. 2001

MBoC

View full text Add to dashboard Cite

Macrophage stimulating protein (MSP), also known as hepatocyte growth factor-like, is a soluble cytokine that belongs to the family of the plasminogen-related growth factors (PRGFs). PRGFs are ␣/␤ heterodimers that bind to transmembrane tyrosine kinase receptors. MSP was originally isolated as a chemotactic factor for peritoneal macrophages. Through binding to its receptor, encoded by the RON gene, it stimulates dissociation of epithelia and works as an inflammatory mediator by repressing the production of nitric oxide (NO). Here, we identify a novel role for MSP in the central nervous system. As a paradigm to analyze this function we chose the hypoglossal system of adult mice. We demonstrate in vivo that either administration of exogenous MSP or transplantation of MSP-producing cells at the proximal stump of the resected nerve is sufficient to prevent motoneuron atrophy upon axotomy. We also show that the MSP gene is expressed in the tongue, the target of the hypoglossal nerve, and that MSP induces biosynthesis of Ron receptor in the motoneuron somata. Finally, we show that MSP suppresses NO production in the injured hypoglossal nuclei. Together, these data suggest that MSP is a novel neurotrophic factor for cranial motoneurons and, by regulating the production of NO, may have a role in brain plasticity and regeneration. INTRODUCTIONMacrophage stimulating protein (MSP;Skeel et al., 1991;Yoshimura et al., 1993), also known as hepatocyte growth factor-like (Degen et al., 1991), belongs to the family of plasminogen-related growth factors (PRGF; Donate et al., 1994), of which hepatocyte growth factor (HGF) is the prototype. These soluble cytokines share a common structure: they are heterodimeric polypeptides comprising two subunits joined by disulfide bonds, respectively, characterized by the presence of four kringle domains and a nonfunctional serine-protease-like domain (Naldini et al., 1992;Waltz et al., 1997). The PRGF receptors of MSP and HGF identified so far are Ron for MSP (Gaudino et al., 1994) and Met for HGF (Bottaro et al., 1991;Naldini et al., 1991).MSP/hepatocyte growth factor-like was originally isolated as a chemotactic factor for peritoneal macrophages Skeel, 1978, 1979), although it may also act as a mitogen or morphogen in a variety of other cell types such as osteoclasts (Kurihara et al., 1996(Kurihara et al., , 1998, epithelial cells (Medico et al., 1996), hematopoietic precursors (Broxmeyer et al., 1996), and carcinoma cells (Maggiora et al., 1998;Willett et al., 1998). It has been shown that in exudate macrophages MSP inhibits the production of nitric oxide (NO), an inflammatory mediator produced in response to treatment with bacterial lipopolysaccharide or interferon-gamma (Wang et al., 1994;Chen et al., 1998).MSP transcripts are present in the liver and, at a lower amount, in kidney and pancreas (Bezerra et al., 1993;Yoshimura et al., 1993). Transcripts of RON (also known as STK in mouse) are detectable in many different organs during murine development. Relatively late in development, hig...

show abstract

Structure and Biosynthesis of Nerve Growth Factor

Fahnestock

1991

Current Topics in Microbiology and Immunology

View full text Add to dashboard Cite

Expression of the alpha subunit of 7S nerve growth factor in the mouse submandibular gland

Cited by 8 publications

References 35 publications

The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

The sequence of a cDNA done coding for a novel kallikrein from mouse submaxillary gland

Macrophage Stimulating Protein Is a Novel Neurotrophic Factor

Structure and Biosynthesis of Nerve Growth Factor

Contact Info

Product

Resources

About