Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Basyuni, Mohammad; Baba, Shigeyuki; Inafuku, Masashi; Iwasaki, Hiromichi; Kinjo, Kazutoshi; Oku, Hirosuke

doi:10.1016/j.jplph.2009.05.008

Cited by 53 publications

(46 citation statements)

References 53 publications

(81 reference statements)

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“…Furthermore, previous works have demonstrated that the expression PgTPS terpene synthase increased by salt stress in Panax ginseng [14]. Our previous studies also showed salinity-dependent increases in the content of triterpenes and triterpene synthase gene expression in both secreting and non-secreting mangrove roots and leaves [6][7][8][15][16]. It has been disputed for a long time whether triterpene synthases ensuing different products are discrete proteins or if they are produced post-translational from one gene product to another [3].…”

Section: Phyre2 and Swiss-protein Modelsupporting

confidence: 67%

“…Changes in the membrane lipid composition and properties represent an essential factor in the adaptation to high salt concentration [18]. These observations strongly suggested that the triterpene and triterpene synthase contribute to the salt tolerance of mangrove plants probably by changing the property of the cell membrane [6][7][8].…”

Section: Phyre2 and Swiss-protein Modelmentioning

confidence: 91%

“…Molecular mechanism of salinity tolerance in mangrove plants has been described: the level mRNA of triterpene synthase genes of mangrove leaves and roots increased with increasing salt concentration [6][7][8]. These studies suggested that triterpenes play an essential physiological role in the adaptation of mangroves trees as the self-protecting barrier against the external salt stress.…”

Section: Introductionmentioning

confidence: 99%

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Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

Basyuni

Wati

Sulistiyono

et al. 2018

J. Phys.: Conf. Ser.

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Abstract. Molecular cloning of five oxidosqualene cyclases (OSC) genes from Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa had previously been cloned, characterized, and encoded mono and -multi triterpene synthases. The present study analyzed protein modelling of triterpene synthase genes from mangrove using Phyre2 and Swiss-model. The diversity was noted within protein modelling of triterpene synthases using Phyre2 from sequence identity (38-43%) and residue (696-703). RsM2 was distinguishable from others for template structure; it used lanosterol synthase as a template (PDB ID: w6j.1.A). By contrast, other genes used human lanosterol synthase (1w6k.1.A). The predicted bind sites were correlated with the product of triterpene synthase, the product of BgbAS was -amyrin, while RsM1 contained a significant amount of -amyrin. Similarly BgLUS and KcMS, both main products was lupeol, on the other hand, RsM2 with the outcome of taraxerol. Homology modelling revealed that 696 residues of BgbAS, BgLUS, RsM1, and RsM2 (91-92% of the amino acid sequence) had been modelled with 100% confidence by the single highest scoring template using Phyre2. This coverage was higher than Swiss-model (85-90%). The present study suggested that molecular cloning of triterpene genes provides useful tools for studying the protein modelling related regulation of isoprenoids biosynthesis in mangrove forests.

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Section: Phyre2 and Swiss-protein Modelsupporting

confidence: 67%

Section: Phyre2 and Swiss-protein Modelmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

Basyuni

Wati

Sulistiyono

et al. 2018

J. Phys.: Conf. Ser.

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“…Among these remarkably differentially expressed genes, only 32.5 % up-regulated and 3.3 % down-regulated genes were co-regulated in upper and lower leaves, as well as in roots (Miyama and Hanagata 2007). Basyuni et al (2009) studied the molecular mechanisms of salinity tolerance in the halophytic K. candel and B. gymnorrhiza roots. mRNA level of multifunctional terpenoid synthase KcMS was increased with salt concentration in both roots and leaves of K. candel.…”

Section: Salinity Balancementioning

confidence: 99%

Mangrove root: adaptations and ecological importance

Srikanth

Lum

Chen

2015

Trees

136

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Key message This review gives a comprehensive overview of adaptations of mangrove root system to the adverse environmental conditions and summarizes the ecological importance of mangrove root to the ecosystem. Abstract In plants, the first line of defense against abiotic stress is in their roots. If the soil surrounding the plant root is healthy and biologically diverse, the plant will have a higher chance to survive in stressful conditions. Different plant species have unique adaptations when exposed to a variety of abiotic stress conditions. None of the responses are identical, even though plants have become adapted to the exact same environment. Mangrove plants have developed complex morphological, anatomical, physiological, and molecular adaptations allowing survival and success in their high-stress habitat. This review briefly depicts adaptive strategies of mangrove roots with respect to anatomy, physiology, biochemistry and also the major advances recently made at the genetic and genomic levels. Results drawn from the different studies on mangrove roots have further indicated that specific patterns of gene expression might contribute to adaptive evolution of mangroves under high salinity. We also review crucial ecological contributions provided by mangrove root communities to the ecosystem including marine fauna.

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“…n.a: no activity (≤5%); EW, extract weight; GAE, gallic acid equivalents; Asc AE equivalents, mg ascorbic acid/g sample. *concentration tested: 1.6 mg.mL -1 presence of unsaturated acids dramatically decreases, indicating a desperate effort of the plant to maintain its integrity [55].…”

Section: Antioxidant Activitymentioning

confidence: 97%

How Salt Stress Represses the Biosynthesis of Marrubiin and Disturbs the Antioxidant Activity of Marrubium Vulgare L

Rezgui¹,

Majdoub²,

Ben-Kaab³

et al. 2017

Pol. J. Environ. Stud.

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Expression of terpenoid synthase mRNA and terpenoid content in salt stressed mangrove

Cited by 53 publications

References 53 publications

Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

Protein modelling of triterpene synthase genes from mangrove plants using Phyre2 and Swiss-model

Mangrove root: adaptations and ecological importance

How Salt Stress Represses the Biosynthesis of Marrubiin and Disturbs the Antioxidant Activity of Marrubium Vulgare L

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