How Salt Stress Represses the Biosynthesis
of Marrubiin and Disturbs the Antioxidant Activity
of Marrubium Vulgare L

Rezgui, M.; Majdoub, Nessrine; Ben-Kaab, Sofiene; Marzouk, Brahim; Gouia, Houda; Araújo, Maria Eduarda; Ben-Kaab, Leila Bettaieb

doi:10.15244/pjoes/64792

Cited by 10 publications

(13 citation statements)

References 47 publications

(67 reference statements)

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“…The identity of marrubiin was checked by FTIR, 1 H, and 13 C NMR. Spectroscopic data were identical to the literature [23]. To quantify the amount of marrubiin in different samples, a calibration curve was performed with known amounts of marrubiin, according to the method previously described [23].…”

Section: Isolation Of Marrubiin and Quantification Of Marrubiin Labdanoidsmentioning

confidence: 99%

Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Rezgui

Basma

Neng

et al. 2021

Foods

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Marrubium vulgare L., known as horehound, is a widespread and widely known plant that is used in beer breweries and also as a traditional remedy in Tunisia. In this study, methanolic extracts of plants harvested from five different locations were investigated for their antioxidant activities using three assays (ferric reducing power, radical scavenging activity, and β-carotene-linoleic acid bleaching assay) as well as the total phenolic content. The mineral composition of the plant was also investigated concerning the following elements: Fe, Mg, Ca, Cu, Zn, Mn, K, and three heavy metals, Ni, Pb, and Cd. Marrubiin, the major bioactive diterpenoid lactone, was quantified by NMR in the samples. The essential oils were obtained by hydrodistillation, and their radical scavenging activity was investigated. The toxicity of essential oils was evaluated against Artemia salina (the brine shrimp larva). The essential oil showed a weak radical scavenging activity and low toxicity. Data obtained from the five different locations showed that the antioxidant activity, as well as the total phenolic and marrubiin content, were strongly affected by the harvest sites. The metal content in the samples showed differences with the harvest location, but there was always a great abundance of calcium, magnesium, and potassium.

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Section: Isolation Of Marrubiin and Quantification Of Marrubiin Labdanoidsmentioning

confidence: 99%

Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Rezgui

Basma

Neng

et al. 2021

Foods

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“…Other results [ 12 ] showed that salt treatment (different concentrations of NaCl) affects the morphological, physiological, and biochemical traits of M. vulgare , which appears to be highly sensitive to salinity, especially the content of the main bioactive compound marrubiin, which decreased with higher NaCl concentration.…”

Section: Botanical and Agronomical Featuresmentioning

confidence: 99%

“…In Spain, M. vulgare has been popularly used on chicken farms to prevent lice and frequent scratching of animals, which intensified its planting on farms [ 12 ]. Moreover, a plant extract of M. vulgare was tested against fourth instar larvae of the mosquito Culex pipiens .…”

Section: Applications In Medicine and As Pesticidesmentioning

confidence: 99%

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

et al. 2020

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Marrubium vulgare is a plant with high bioactive potential. It contains marrubiin, a labdane diterpene that is characteristic for this genus, as well as a complex mixture of phenolic compounds. According to numerous studies, M. vulgare acts as a good antioxidant agent, and due to this, it could potentially be useful in treatments of cancer, diabetes mellitus, and liver diseases. In addition, its anti-inflammatory, wound-healing, antihypertensive, hypolipidemic, and sedative potential are discussed. Apart from that, its antimicrobial activity, especially against Gram+ bacteria, fungi, herpes simplex virus, and parasites such as Toxoplasma gondii, Trichomonas vaginalis, and Plasmodium berghei-berghei was recorded. Additionally, it could be used as a chicken lice repellent, herbicide, and natural insecticide against mosquito larvae and natural molluscicide. In veterinary medicine, M. vulgare can be used as an anthelmintic against the eggs and larvae of bovine strongyles parasites, and as an antibiotic against bovine mastitis caused by resistant bacterial strains. Due to the mentioned benefits, there is a tendency for the cultivation of M. vulgare in order to ensure high-quality raw material, but more firm scientific evidence and well-designed clinical trials are necessary for the well-established use of M. vulgare herb and its preparations.

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“…Overall, impaired absorption of important ions and elements such as calcium and nitrogen, and closed stomata to prevent water loss reduce water uptake from the soil affecting the plant growth (Cramer, 2002;Bhatt and Rao, 2005). Previous results have also shown that shoot length fresh weight of B. nigra decreases significantly due to increasing salt concentration (Rezgui et al, 2017).…”

Section: Resultsmentioning

confidence: 87%

Effect of Salinity on Morphological Characteristics, Biochemical Accumulation, and Essential Oil Yield and Composition in Black Horehound (<em>Ballota nigra</em> L.)

Mehdikhanlou¹,

Dibazarnia²,

Oustan³

et al. 2021

Preprint

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Black horehound (Ballota nigra L.) is one of the important medicinal plants, which is a rich source of health-promoting essential oils. Salinity stress affects plant development and alters the quality and quantity of plants extracts and their composition. This study was aimed to investigate the effect of salinity on morphological, physiological characteristics, and secondary metabolites of B. nigra under greenhouse, and in vitro culture conditions. The plants were treated with different concentrations of NaCl (25, 50, 75, 100 mM) and fresh and dry weight of leaf and stem were measured as well as morphological characteristics of the plant. Plant growth was reduced with the increased salinity concentrations. The results showed that all growth-related traits and SPAD were decreased both in vivo and in vitro. Additionally, increased salt concentration affected the cell membrane integrity. Total phenolics content of plants growing in the greenhouse, increased by 21% at 50 mM NaCl, but at higher stress levels (100 mM NaCl), the amounts were decreased significantly. Total flavonoids contents followed similar patterns, with a slight difference. In addition, the maximum and minimum total phenolics contents of plants growing under in vitro condition were observed at 50 mM NaCl and control treatments, respectively. Increasing the salt concentration significantly affected the total flavonoids content, and as a result, the highest amount was observed in 50 and 75 mM NaCl treatments. Antioxidant activity was also measured. Among the NaCl treatments, the highest DPPH scavenging activities (IC50) under greenhouse and in vitro conditions were detected at 50 mM and 25 mM concentrations, respectively. In general, based on the results, with increasing the salinity level to 75 mM, the activities of CAT and APX were significantly upregulated in both greenhouse and in vitro culture conditions. A correlation between total phenolics and flavonoids contents as well as antioxidant activity were obtained. With shifting salinity stress, the type and the amount of the identified essential oil compounds changed. Compounds such as styrene, tridecanol, germacrene-D, beta-Ionone, beta-bisabolene, and caryophyllene oxide increased compared to the controlled treatment.

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How Salt Stress Represses the Biosynthesis of Marrubiin and Disturbs the Antioxidant Activity of Marrubium Vulgare L

Cited by 10 publications

References 47 publications

Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Evaluation of Marrubium vulgare Growing Wild in Tunisia for Its Potential as a Dietary Supplement

Marrubium vulgare L.: A Phytochemical and Pharmacological Overview

Effect of Salinity on Morphological Characteristics, Biochemical Accumulation, and Essential Oil Yield and Composition in Black Horehound (<em>Ballota nigra</em> L.)

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