Expression of Ten‐m/Odz3 in the fibrous layer of mandibular condylar cartilage during postnatal growth in mice

Abstract

It has been speculated that the mandibular condyle develops via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes; however, the developmental mechanisms behind this process have not been revealed. We used laser-capture microdissection and cDNA microarray analysis to elucidate the genes that are highly expressed in these fibroblast-like cells. Among these genes, the transcription of Ten-m ⁄ Odz3 was significantly increased in the fibroblast-like cells compared with other cartilage tissues. For the first time, we describe the temporal and spatial expression of Ten-m ⁄ Odz3 mRNA in relation to the expression of type I, II, and X collagen mRNA, as determined by in-situ hybridization in mouse mandibular condylar cartilage and mouse femoral cartilage during the early stages of development. Ten-m ⁄ Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, which expressed type I and II collagen, respectively, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in-vitro expression of Ten-m ⁄ Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m ⁄ Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m ⁄ Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.

Novel mutation in Teneurin 3 found to co‐segregate in all affecteds in a multi‐generation family with developmental dysplasia of the hip

“…The Tenm3 gene encodes a transmembrane protein thought to function as a cell signal transducer. It is expressed in the developing murine nervous system and in the femoral cartilage of 1 day old mice …”

Acute Rejection of Knee Joint Articular Cartilage in a Rat Composite Tissue Allotransplantation Model

“…This method can overcome the problem of tissue heterogeneity by selecting defined cells to minimize contamination from neighboring unrelated tissues, providing a reliable assessment of tissue-specific gene expression. A few previous studies have involved selection of chondrocytes from skeletal tissue and analysis of gene expression by means of a qRT-PCR or microarray method [20][21][22][23][24] . Although our results indicated that expression of the caspase-3 gene was similar in allografts and isografts shortly after transplantation, we found a subsequent decrease in caspase-3 expression in the allografts over time, which was significant from postoperative day seven onward.…”

The Intracellular Domain of Teneurin-1 Induces the Activity of Microphthalmia-associated Transcription Factor (MITF) by Binding to Transcriptional Repressor HINT1

“…Finally, we cannot exclude the possibility that our identified mechanism takes place in a non-neuronal context. Recent papers have identified teneurin-3 and -4 as important players in chondrogenic differentiation (49,50). Although teneurin-1 has not been studied in chondrogenesis, the likely role of GPNMB as a key regulator in chondrogenesis could implicate a function of both proteins in this event.…”