Expression of STK39 in peripheral blood of hypertension patients and the relationship between its genetic polymorphism and blood pressure

Abstract

ABSTRACT. This study investigated the STK39 expression in peripheral blood of hypertension patients and the relation between its genetic polymorphism and blood pressure. The observation group comprised of 42 primary hypertension patients admitted to our hospital, and the control group comprised of 30 healthy individuals who underwent physical examination in our hospital during the same period. Fasting venous blood was collected from both groups in the morning to determine the STK39 mRNA and protein levels in peripheral blood using quantitative real-time PCR and western blot. STK39 gene SNP (rs6433027) was sequenced using PCR and its genetic variation was analyzed. The relationship between STK39 protein level, genetic variation, and diastolic and systolic blood pressure was also analyzed. The observation group showed increased STK39 mRNA and protein levels in peripheral blood compared to the control group, and the difference was statistically significant (P < 0.05), suggesting C/T mutation 16461-16468 (2015) in STK39 gene SNP (rs6433027). Correlation analysis showed positive association between STK39 protein level and diastolic and systolic blood pressure (P < 0.05), indicating a positive association between C/T genetic mutation and diastolic and systolic blood pressure (P < 0.05). In conclusion, STK39 mRNA and protein express abnormally in primary hypertension patients with genetic variation, which is related to the blood pressure.

An epigenome-wide study of body mass index and DNA methylation in blood using participants from the Sister Study cohort

“…Flanking CpGs on the 450K array (cg21899461 and cg03331300) were also associated with BMI at p<0.05. Polymorphisms in this gene have been linked to risk of hypertension (33-35). Epigenetic silencing of STK39 through DNA hypermethylation in B-cell lymphoma appears to promote cancer progression by inhibiting apoptosis in cells with DNA damage (36).…”

Association of with‐no‐lysine kinase 1 and Serine/Threonine kinase 39 gene polymorphisms and haplotypes with essential hypertension in Tibetans

“…Essential hypertension (EH) is the most significant modifiable risk factor for CVD, accounting for 208.1 million DALYs and 10.4 million deaths worldwide [Forouzanfar et al, ]. However, hypertension prevalence differs across different geographical regions and minorities in China [Gu et al, ; Li et al, ]. According to the China Health and Nutrition Survey (CHNS), Tibetans had the highest prevalence of hypertension both in males and females, at 25.6% and 24.0% respectively [Hu et al, ], and the number was reported even up to 55.9% [Zhao et al, ].…”

“…Serine/Threonine kinase 39 ( STK39 ), encoding SPAK, spans about 300 kb on chromosome 2q24.3 with 34 polymorphisms [Zhao et al, ]. Both WNK1 and STK39 have had several genetic polymorphisms been reported that contribute to hypertension incidence [Putku et al, ; Li et al, ], yet none of them had been studied in Tibetans.…”