Expression of small heat shock protein (sHSP) genes in the garden pea (<i>Pisum sativum</i>) under slow horizontal clinorotation

Talalaiev, Oleksandr; Korduym, Elizabeth

doi:10.4161/psb.29035

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Chaperones also maintain crosstalk with signaling molecules, antioxidants (acerbate peroxidase), and osmolytes (trehalose, proline, glycine betaine) (Wang et al, 2004;Kang et al, 2022). Various reports have confirmed accumulation of all HSP families in different vegetables and food legumes under HS, with greater accumulation of sHSPs than other HSPs, as reported for spinach (Guy and Li, 1998), tomato (Preczewski et al, 2000), soybean (Ortiz and Cardemil, 2001), common bean and cowpea (Simões-Araújo et al, 2003), potato (Ahn et al, 2004), cabbage (Park et al, 2013), pea (Talalaiev and Korduym, 2014), faba bean (Kumar et al, 2015), capsicum , chickpea (Meena et al, 2017), and broccoli (Lin et al, 2019). Accumulation of these proteins helps plants to re-establish homeostasis under HS conditions.…”

Section: Defense Responsesmentioning

confidence: 89%

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

et al. 2022

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Vegetables are a distinct collection of plant-based foods that vary in nutritional diversity and form an important part of the healthy diet of the human being. Besides providing basic nutrition, they have great potential for boosting human health. The balanced consumption of vegetables is highly recommended for supplementing the human body with better nutrition density, dietary fiber, minerals, vitamins, and bioactive compounds. However, the production and quality of fresh vegetables are influenced directly or indirectly by exposure to high temperatures or heat stress (HS). A decline in quality traits and harvestable yield are the most common effects of HS among vegetable crops. Heat-induced morphological damage, such as poor vegetative growth, leaf tip burning, and rib discoloration in leafy vegetables and sunburn, decreased fruit size, fruit/pod abortion, and unfilled fruit/pods in beans, are common, often rendering vegetable cultivation unprofitable. Further studies to trace down the possible physiological and biochemical effects associated with crop failure reveal that the key factors include membrane damage, photosynthetic inhibition, oxidative stress, and damage to reproductive tissues, which may be the key factors governing heat-induced crop failure. The reproductive stage of plants has extensively been studied for HS-induced abnormalities. Plant reproduction is more sensitive to HS than the vegetative stages, and affects various reproductive processes like pollen germination, pollen load, pollen tube growth, stigma receptivity, ovule fertility and, seed filling, resulting in poorer yields. Hence, sound and robust adaptation and mitigation strategies are needed to overcome the adverse impacts of HS at the morphological, physiological, and biochemical levels to ensure the productivity and quality of vegetable crops. Physiological traits such as the stay-green trait, canopy temperature depression, cell membrane thermostability, chlorophyll fluorescence, relative water content, increased reproductive fertility, fruit numbers, and fruit size are important for developing better yielding heat-tolerant varieties/cultivars. Moreover, various molecular approaches such as omics, molecular breeding, and transgenics, have been proved to be useful in enhancing/incorporating tolerance and can be potential tools for developing heat-tolerant varieties/cultivars. Further, these approaches will provide insights into the physiological and molecular mechanisms that govern thermotolerance and pave the way for engineering “designer” vegetable crops for better health and nutritional security. Besides these approaches, agronomic methods are also important for adaptation, escape and mitigation of HS protect and improve yields.

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Section: Defense Responsesmentioning

confidence: 89%

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

et al. 2022

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“…Wood et al (1998) exposed peas plants to HTemp (37°C for 6 h) that accumulated two low molecular weight (LMW) HSPs (22 kDa). Talalaiev and Korduym (2014) determined the effects of temperature on the expression of HSPs of peas and conform extreme sensitivity of these genes to HS tolerance. Most of the highly induced genes include ER-localized Pshsp22.7, mitochondrial Pshsp22.9 and chloroplast Pshsp26.2 so that the expression of these genes increased up to several thousand-fold relative to the controlled seedlings at 42°C.…”

Section: Synthesis Of Heat Shock Protein/factorsmentioning

confidence: 99%

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

Devi

Sagar²,

Mishra³

et al. 2023

Front. Plant Sci.

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In the era of climate change, the overall productivity of pea (Pisum sativum L.) is being threatened by several abiotic stresses including heat stress (HS). HS causes severe yield losses by adversely affecting several traits in peas. A reduction in pod yield has been reported from 11.1% to 17.5% when mean daily temperature increase from 1.4 to 2.2°C. High-temperature stress (30.5-33°C) especially during reproductive phase is known to drastically reduce both seed yield and germination. HS during germination and early vegetative stage resulted in poor emergence and stunted plant growth along with detrimental effects on physiological functions of the pea plant. To combat HS and continue its life cycle, plants use various defense strategies including heat escape, avoidance or tolerance mechanisms. Ironically, the threshold temperatures for pea plant and its responses are inconsistent and not yet clearly identified. Trait discovery through traditional breeding such as semi leaflessness (afila), upright growing habit, lodging tolerance, lower canopy temperature and small seeded nature has highlighted their utility for greater adaptation under HS in pea. Screening of crop gene pool and landraces for HS tolerance in a targeted environment is a simple approach to identify HS tolerant genotypes. Thus, precise phenotyping using modern phenomics tools could lead to increased breeding efficiency. The NGS (next generation sequencing) data can be associated to find the candidate genes responsible for the HS tolerance in pea. In addition, genomic selection, genome wide association studies (GWAS) and marker assisted selection (MAS) can be used for the development of HS tolerant pea genotypes. Additionally, development of transgenics could be an alternative strategy for the development of HS tolerant pea genotypes. This review comprehensively covers the various aspects of HS tolerance mechanisms in the pea plant, screening protocols, omic advances, and future challenges for the development of HS tolerant genotypes.

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Development and validation of an undergraduate laboratory activity exploring the dna analysis of pet food

Waldo

Pereira

Rahman

et al. 2018

Biochem Molecular Bio Educ

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A novel experiment has been developed with a goal of enhancing engagement and deepening understanding of fundamental molecular biology concepts. In this laboratory activity, students investigate the composition of pet foods through quantitative polymerase chain reaction (qPCR) analysis. By conducting this experiment, students address a scientific question that has the potential to be of personal interest and they gain an appreciation of the power of genetic analysis. To develop this activity, ingredient lists of popular pet foods were surveyed and eight different components were selected to be the subject of inquiry. Reaction conditions for qPCR were validated and optimized, and a simple, efficient, and student‐friendly protocol was developed. A cohort of upper‐level biology students extracted DNA from pet foods and conducted the qPCR reactions. Specificity of amplification was determined by conducting melting point analysis and agarose gel electrophoresis of the products. Student reactions were analyzed and efficacy of this approach for deepening understanding of fundamental biochemical and molecular biology concepts was documented. © 2018 International Union of Biochemistry and Molecular Biology, 46(5):536–546, 2018.

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Expression of small heat shock protein (sHSP) genes in the garden pea (Pisum sativum) under slow horizontal clinorotation

Cited by 4 publications

References 20 publications

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

Physiological and Molecular Approaches for Developing Thermotolerance in Vegetable Crops: A Growth, Yield and Sustenance Perspective

Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward

Development and validation of an undergraduate laboratory activity exploring the dna analysis of pet food

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