Expression of selected proteins in breast cancer brain metastases

Abstract

Abstract. The aim of the study was to assess the immunohistochemical (IHC) profiles of SRC3, Pax2, ER, PgR, Her2, EGFR, CK5/6, and Ki67 proteins in breast-cancer brain metastasis. The study utilized tumor samples from 30 metastatic patients and calculated correlations between all IHC variables. In fourteen cases, primary breast cancers paired with secondary deposits were analyzed. We evaluated the association between IHC status in the primary and secondary deposits, grade, and histotype of the tumors. The examination of the metastatic deposits in all 30 patients resulted in positive detection in the following cases: SRC3 in 20 cases (66.6%), Pax2 in 22 (73.3%), ER in 22 (73.3%), PgR in 25 (83.3%), Her2 in 10 (33.3%), EGFR in 12 (40%), CK5/6 in 7 (23.3%), and Ki67 in 23 (76.6%). Grade 2 was found in 13.3% of all patients, and grade 3 in 86.7%. SRC3 and Pax2 were positive in both G2 and G3. Invasive lobular carcinoma and invasive ductal carcinoma were diagnosed in 23.3% and 76.7% of cases, respectively. There were no differences between the IHC expression of the studied proteins in either grading or histotype of the tumors. In the IHC profiles, which included SRC3, Pax2, ER, PgR, Her2, CK5/6, Ki67, and EGFR, we found no statistically significant differences between the primary cancer and the brain metastasis. In our study of metastatic breast carcinoma deposits, there was no correlation between SRC3, Pax2 status and histotype, and tumor grade. The IHC status of the paired primary and metastatic deposits did not differ in a statistically significant manner.

A combinational therapy of EGFR-CAR NK cells and oncolytic herpes simplex virus 1 for breast cancer brain metastases

“…With regard to breast cancer, Gojis et al reported 30 cases of breast cancer patients with metastasis and found positive wtEGFR protein expression in 12 patients (40%). There was no statistically significant difference of EGFR expression between primary cancer and brain metastasis [ 6 ]. Since the EGFR-CAR NK-92 cells that we generated were able to target both wtEGFR and EGFRvIII, they will be more broadly applicable than agents targeting EGFRvIII alone, particularly in the setting of BCBMs.…”

“…The main challenge for successful use of CAR NK cells and CAR T cells is to find a proper surface antigen to target. EGFRs are highly expressed in a majority of BCBM patients [ 6 ]. In addition to being present on some breast cancer stem cells (CSCs), EGFR plays an important role in cell proliferation, motility, and survival in various tumors including breast cancer [ 7 ].…”

P53 mutation compared with Ki67 marker in metastasis of breast cancer in western Iran

“…In this study in patients with metastasis, 16 patients (69.5%) had Ki67>10% in tumors. Also, in patients with Ki67 positive, more patients didn't have metastasis, and there is no significant relationship between Ki67 with metastasis (P>0.05) such as Gojis O and et al [21] , and Tvedskov TF and et al [22] . Some of other studies [23][24][25][26] showed that Ki67 had a significant relationship with metastasis.…”

Skull bone metastasis with adjacent leptomeningeal involvement from pleomorphic lobular carcinoma of the breast

“…In identifying the breast origin of an SBM, ER positivity may be useful, as many SBMs are luminal cancers. However, loss of ER expression could occur in metastatic sites, including bone, and a 28–42% rate of discordance of ER expression between the primary and metastatic lesions has been reported . This fact should be borne in mind in the work‐up of SBM with an unknown primary.…”

“…The current case is an infiltrating pleomorphic lobular carcinoma giving rise to a solitary SBM with both dural and leptomeningeal involvement by direct extension. Breast cancer is the most common primary tumour associated with intracranial metastasis, with dura‐based metastases more likely to arise from infiltrating duct cancer, no special type, and leptomeningeal meatastases more likely to arise from infiltrating lobular carcinoma . As most of these histological types are luminal, the reason for the site predilection is uncertain.…”