Expression of Rhodococcus opacus alkB genes in anhydrous organic solvents

Sameshima, Yuka; Honda, Kohsuke; Kato, Junichi; Ômasa, Takeshi; Ohtake, Hisao

doi:10.1263/jbb.106.199

Cited by 30 publications

(36 citation statements)

References 20 publications

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“…The inability of alkane-degrading Rhodococcus strains to grow on certain range of n-alkanes has also been described by Sameshima et al [311] in Rhodococcus opacus B4. B4 cells could not utilize n-pentane (C 5 ) and n-hexane (C 6 ) because of the short-chain length n-alkane toxicity [311]. Another example regards a propane utilizer, R. rhodochrous PNKbl, that was isolated by Woods and Murrell [330] and it was shown to be unable to grow on short-chain n-alkanes ranging from C 1 to C 8 .…”

Section: Discussionmentioning

confidence: 56%

“…The impossibility for BCP1 to grow in liquid medium in the presence of one of these n-alkanes together with n-hexane, suggested a toxicity factor putatively correlated with the carbon chain length that can cause problems in membrane structure and physiology properties [329]. The inability of alkane-degrading Rhodococcus strains to grow on certain range of n-alkanes has also been described by Sameshima et al [311] in Rhodococcus opacus B4. B4 cells could not utilize n-pentane (C 5 ) and n-hexane (C 6 ) because of the short-chain length n-alkane toxicity [311].…”

Section: Discussionmentioning

confidence: 72%

“…B4 alkB1 has been reported to be induced by the presence of n-alkanes; conversely, Rh. B4 alkB2 expression did not seem to be affected by these compounds [311]. Both B4 AlkB1 and AlkB2 amino acid sequences showed high homology with BCP1 AlkB (AlkB1 84% and AlkB2 81%); thus, it was hypothesized that the comparison of these alkB upstream regions with the BCP1 PalkB could allow to distinguish between the conserved sequences involved in the alkB gene induction promoted by the presence of n-alkanes (regions conserved between BCP1 alkBup and B4 alkB1up) and the conserved sequences useful for the rhodococcal transcriptional apparatus which is not dependent on n-alkanes (regions conserved between BCP1 alkBup and B4 alkB2up).…”

Section: On the Promoter Of Alkb Genementioning

confidence: 82%

“…The alkB genes have been genetically described in a relative little number of Rhodococcus strains including Rhodococcus opacus B4 [311] and Rhodococcus strains NRRL B-16531 and Q15 [195] whereas the complete alkB operons of Rhodococcus erythropolis PR4, Rhodococcus jostii RHA1 [257], Rhodococcus erythropolis strain T7-2 [339] and Rhodococcus erythropolis SK121 [294] are available in the database but they have not been further described.…”

Section: Discussionmentioning

confidence: 99%

“…As results, only alkB1 gene expression seemed to be induced by the growth on n-alkanes and its expression level was also good in anhydrous solvent. This result is considered to have practical significance for the development of new bioconversion processes using waterimmiscible chemicals [311].…”

Section: Alkane Metabolism In Rhodococcus Strainsmentioning

confidence: 98%

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Monooxygenases involved in the n-alkanes metabolism by Rhodococcus sp. BCP1: molecular characterization and expression of alkB gene

Cappelletti

Fedi

Honda

et al. 2010

Journal of Biotechnology

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Discussion 107 Summary 113Chapter 5 -Analysis of the alkB gene expression Summary 157Chapter 6 -Proteomic analysis of n-alkanes growth of Rhodococcus sp. BCP1 Chapter 1 -General Introduction PrefaceThe quality of life on Earth is tightly related with the overall quality of the environment [1]. During the last century it was commonly believed that the abundance of land and resources were unlimited and it was rarely acknowledged that the production, use, and disposal of hazardous substances had environmental and health effects [1][2][3]. Owing to this, human activity has deliberately or inadvertently released into waters and soil a variety of toxic compounds as refrigerants, paints, solvents, herbicides and pesticides that can cause considerable environmental pollution and human health problems as a result of their persistence, toxicity, and transformation into hazardous metabolites [4,5]. Very soon, however, it became clear how the carelessness and negligence in handling the industrial wastes and gas emissions had caused dramatic effect on the nature and on human health [1].Nowadays, the concern about the global warming and the climate changing induced the government regulatory agencies to establish procedures for assessing the environmental impact and health hazards of chemicals and for controlling/limiting their utilization.International efforts have been applied to remedy many contaminated sites all over the world, either as a response to the risk of adverse health or environmental effects caused by contamination or to enable the site to be redeveloped for use [5]. In response to public and government concern and because of the intriguing research problems presented, environmental scientists, biologists and chemists have been giving increased attention to identifying and determining the behavior and fate of organic compounds in natural ecosystems [5].Bioremediation procedures offer an economical and effective possibility to degrade or render innocuous toxic pollutants using natural biological activity [1]. By definition, bioremediation implies the use of living organisms, primarily microorganisms, to degrade the Since contaminant compounds are transformed by living organisms through reactions that take place as a part of their metabolic processes, environmental biotechnology focuses on the development of techniques to optimize environmental parameters to allow these metabolic pathways to proceed faster and, therefore, to improve the bio-degradation [1]. As a result, the removal of the contaminated soil can be necessary to let the biodegradation proceed in controlled sites (such as bioreactors). These ex situ approaches provide optimal conditions out one single step of the entire bio-degradation process. Hydrocarbons in the environmentHydrocarbons are energy-rich organic compounds consisting of carbon and hydrogen atoms and they can be saturated (only single C-H bounds) or unsaturated (one ore more double or triple C-H bounds). Alkanes are saturated hydrocarbons with the general formula C n H 2n+2 ; they can b...

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Section: Discussionmentioning

confidence: 56%