“…Sections (5 m) were incubated overnight with primary antibodies against amylase (polyclonal, 1/5,000; Sigma-Aldrich), glucagon (polyclonal, 1/3,000; Dr. C. Van Schravendijk, Vrije Universiteit Brussel, Brussels), insulin (monoclonal, 1/2,000; Sigma-Aldrich), Ngn3 (polyclonal, 1/1,000; the laboratory of Michael German, University of California, San Francisco), and Pdx1 (polyclonal, 1/1,000; Beta Cell Biology Consortium, Nashville, TN, http://www.betacell.org), and stained as previously described [26].…”