Pluripotent Stem Cell-Derived Pancreatic β Cells: From In Vitro Maturation to Clinical Application

Abdelalim, Essam M.; Emara, Mohamed M.

doi:10.1007/978-3-319-33270-3_6

Cited by 1 publication

(1 citation statement)

References 186 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro pancreatic differentiation protocols have demonstrated that gamma secretase inhibitor (DAPT) treatment enhances the expression of NGN3 by direct inhibition of NOTCH signalling, directing the cells towards an endocrine fate (D'Amour et al ., ; Chen et al ., ; Thatava et al ., ). However, the formation of NGN3‐positive cells was not consistent, as it depends on the cell line used (Abdelalim & Emara, ). Continued inhibition of TGF‐β using TGF‐β type I receptor kinase (Alk5 inhibitor) and BMP signalling (Noggin or Dorsomorphin) following generation of pancreatic progenitors was shown to be essential for induction of hormone‐expressing cells (Shiraki et al ., ) (Fig.…”

Section: Differentiation Of Pluripotent Stem Cells Into Pancreatic β‐mentioning

confidence: 99%

Pathways governing development of stem cell‐derived pancreatic β cells: lessons from embryogenesis

et al. 2017

Self Cite

View full text Add to dashboard Cite

The loss of functional β cells leads to development of diabetes. Several studies have shown that β cells are specified through several stages of progenitors during pancreas development, each stage defined by the expression of specific transcription factors (TFs). Understanding signalling pathways that control the differentiation and specification processes during embryogenesis will facilitate efforts to obtain functional β cells in vitro. Our current knowledge of the mechanisms involved in pancreatic β cell development and survival under normal or diabetic conditions has come largely from animal studies. However, there are marked differences in islet structure and physiological properties between humans and animals, and not all phenotypes of human diabetes can be recapitulated in animal models. Therefore, human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced PSCs (hiPSCs) offer a great opportunity for increasing our understanding of the pathways regulating human pancreatic β-cell development and survival. Furthermore, hPSCs provide a renewable source of functional pancreatic β cells for cell replacement therapy as well as disease modelling. Herein, we discuss the signalling pathways involved in the development of pancreatic β cells during embryogenesis. Additionally, we describe how these pathways are manipulated in vitro to differentiate hPSCs into functional β cells. Finally, we highlight the progress that has been made for the applications of those cells in treating and modelling diabetes.

show abstract

Section: Differentiation Of Pluripotent Stem Cells Into Pancreatic β‐mentioning

confidence: 99%

Pathways governing development of stem cell‐derived pancreatic β cells: lessons from embryogenesis

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Pluripotent Stem Cell-Derived Pancreatic β Cells: From In Vitro Maturation to Clinical Application

Cited by 1 publication

References 186 publications

Pathways governing development of stem cell‐derived pancreatic β cells: lessons from embryogenesis

Pathways governing development of stem cell‐derived pancreatic β cells: lessons from embryogenesis

Contact Info

Product

Resources

About