Expression of Rat Sperm Flagellum-Movement Associated Protein Genes under 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-dioxin Treatment

Yamano, Yoshiaki; Asano, Atsushi; Ohta, Masanori; Hirata, Shuji; Shoda, Tomoko; Ohyama, Kenji

doi:10.1271/bbb.80764

Cited by 6 publications

(6 citation statements)

References 18 publications

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“…It is formed as a by-product in the manufacture of chlorinated hydrocarbons, in the incineration process of municipal waste, paper and pulp bleaching, and in emission from steel foundries and motor vehicles (Skene et al, 1989). The male reproductive system is a sensitive target of TCDD or other dioxin congeners, with effects of TCDD exposure that include increased gonadotropins (e.g., 348 Mustafa Sönmez et al Drug and Chemical Toxicology follicle-stimulating hormone and leutinizing hormone) and decreased testosterone concentrations (Choi et al, 2007), reduced testis, epididymis and accessory gland weights El-Tawil and Elsaieed, 2005), reduced sperm count (Choi et al, 2007), sperm motility (El-Sabeawy et al, 1998;Yamano et al, 2009), increased abnormal sperm rate (Bell et al, 2007), and damage to the normal testicular structure (Rune et al, 1991;El-Sabeawy et al, 1998;Choi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Attenuating effect of lycopene and ellagic acid on 2,3,7,8-tetrachlorodibenzo-p-dioxin–induced spermiotoxicity and testicular apoptosis

Sönmez

Türk

Çeribaşı

et al. 2011

Drug and Chemical Toxicology

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This study was conducted to investigate the prophylactic effects of lycopene (LC) and ellagic acid (EA) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced testicular and spermatozoal toxicity. These toxicological changes are associated with the oxidative stress and apoptosis in male rats. Forty-eight male rats were allocated to one of six groups of 8 rats each: control, LC, EA, TCDD, TCDD+LC, and TCDD+EA. The control group was treated with 0.5 mL/rat slightly alkaline solution+0.5 mL/rat corn oil every other day. The LC group was treated with 0.5 mL/rat slightly alkaline solution+0.5 mL/rat corn oil containing 10 mg/kg of LC every other day. The EA group received 0.5 mL/rat corn oil+0.5 mL/rat slightly alkaline solution containing 2 mg/kg of EA every other day. The TCDD group received 0.5 mL/rat corn oil containing 100 ng/kg/day of TCDD+0.5 mL/rat slightly alkaline solution. The TCDD+LC group was treated with 0.5 mL/rat TCDD+0.5 mL/rat LC. The TCDD+EA group was treated with 0.5 mL/rat TCDD+0.5 mL/rat EA. All treatments were made by gavage, and the experimental period was maintained during 8 weeks. Sperm motility, concentration, and abnormal sperm rate in epididymal tissue, testicular tissue lipid peroxidation (LPO), antioxidant enzyme activity, histopathological changes, and apoptosis (i.e., Bax and Bcl-2 proteins) were determined. TCDD exposure resulted in significant decreases in sperm motility, concentration, testicular superoxide dismutase activity, germinal cell-layer thickness, Johnsen's testicular score, and significant increases in abnormal sperm rate, testicular malondialdehyde, glutathione levels, Bax-positive staining, and Bax-positive apoptotic cell score, along with some testicular histopathological lesions. TCDD treatment did not affect significantly catalase activity. However, combined treatment with LC or EA, in addition to TCDD, prevented the development of TCDD-induced damages in sperm quality, testicular histology, and LPO. Improvements in testicular apoptosis after the administration of LC and EA to TCDD-treated rats were minimal, but not statistically significant. TCDD-induced lipid peroxidation leads to functional and structural damages, as well as apoptosis, in spermatogenic cells of rats. Both LC and EA protected against the development of these effects.

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Section: Introductionmentioning

confidence: 99%

Attenuating effect of lycopene and ellagic acid on 2,3,7,8-tetrachlorodibenzo-p-dioxin–induced spermiotoxicity and testicular apoptosis

Sönmez

Türk

Çeribaşı

et al. 2011

Drug and Chemical Toxicology

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“…We have cloned some of these genes by differential display (DD), and we characterized them according to their prospective functions. [1][2][3][4][5][6][7][8] Several forms of cancer-related gene expression were found to be upregulated during sexual maturation, and these are interesting targets in the attempt to understand the common mechanism between carcinogenesis and spermatogenic stem-cell development and differentiation. We have analyzed the expression of cancer-testis (CT) antigen genes, such as Sdccag8, related to colon cancer, 6) and Tal-1, related to acute T-lymphocytic leukemia in the developing testis.…”

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confidence: 99%

Tumor Suppressor Candidate TUSC3 Expression during Rat Testis Maturation

Khalid

Asano

Hosaka

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…We have cloned and characterized some of these genes by differential display (DD), and we categorized them according to expected functions. [1][2][3][4][5][6] Of these, tumor antigens commonly expressed in normal testis are interesting, because they can be used for cancer diagnosis, and are promising tools for cancer immunotherapy, 7,8) and can be used to understand the process of spermatogenesis. Colon cancer specific antigens have been screened by a recombinant cDNA expression library (SEREX) from an individual cancer patient, and they are promising candidates as cancer-testis (CT) antigen genes.…”

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confidence: 99%