Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation

Khalid, Ahmed Magzoub; Asano, Atsushi; Hosaka, Yoshinao Z.; Ohta, Masanori; Ohyama, Kenji; Yamano, Yoshiaki

doi:10.1271/bbb.120503

Cited by 3 publications

(3 citation statements)

References 36 publications

(51 reference statements)

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“…S6 online). NKX3-1 in the mature testis can cause spermatogenic cell division and differentiation and prompt spermatogenesis if it is suppressed 43 . A previous study demonstrated that increased PTEN may decrease spermatogenesis, fertility, and sperm maturation, and fertilization in the Akt1−/− and Akt2−/− male mice 44 .…”

Section: Discussionmentioning

confidence: 99%

Histological and transcriptome analyses of testes from Duroc and Meishan boars

Ding

Luo

Liu

et al. 2016

Sci Rep

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Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and transcriptome analyses. Haematoxylin-eosin staining was conducted to observe histological structure of the testes in Duroc and Meishan boars at different ages. Although spermatogenesis occurred prior to 75 days in Meishan boars, the number of spermatogonia and Sertoli cells in Meishan boars were less than in Duroc boars at adulthood. The diameters of the seminiferous tubules of the testes differed significantly during the initiation of development of the seminiferous tubules between the two breeds. We obtained differentially expressed functional genes and analysed seven pathways involved in male sexual maturity and spermatogenesis using RNA-seq. We also detected four main alternative splicing events and many single nucleotide polymorphisms from testes. Eight functionally important genes were validated by qPCR, and Neurotrophin 3 was subjected to quantification and cellular localization analysis. Our study provides the first transcriptome evidence for the differences in sexual function development between Meishan and Duroc boars.

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Section: Discussionmentioning

confidence: 99%

Histological and transcriptome analyses of testes from Duroc and Meishan boars

Ding

Luo

Liu

et al. 2016

Sci Rep

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“…We have cloned some of these genes by differential display (DD), and we characterized them according to their prospective functions. [1][2][3][4][5][6][7][8] Several forms of cancer-related gene expression were found to be upregulated during sexual maturation, and these are interesting targets in the attempt to understand the common mechanism between carcinogenesis and spermatogenic stem-cell development and differentiation. We have analyzed the expression of cancer-testis (CT) antigen genes, such as Sdccag8, related to colon cancer, 6) and Tal-1, related to acute T-lymphocytic leukemia in the developing testis.…”

mentioning

confidence: 99%

“…We have analyzed the expression of cancer-testis (CT) antigen genes, such as Sdccag8, related to colon cancer, 6) and Tal-1, related to acute T-lymphocytic leukemia in the developing testis. 8) These are expected to prove to be transcription factors regulating and stimulating downstream target gene expression, leading to carcinogenesis or proper spermatogenesis. We also identified the expression of a tumor suppressor gene, Hrasls5, and it was upregulated in the developing testis.…”

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confidence: 99%

Tumor Suppressor Candidate TUSC3 Expression during Rat Testis Maturation

Khalid

Asano

Hosaka

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Clinical and gonadal transcriptome analysis of 38,XX disorder of sex development pigs

Wu,

Tan,

Zhou

et al. 2024

Biology of Reproduction

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Pigs serve as a robust animal model for the study of human diseases, notably in the context of disorders of sex development (DSD). This study aims to investigate the phenotypic characteristics and molecular mechanisms underlying the reproductive and developmental abnormalities of 38,XX ovotestis-DSD (OT-DSD) and 38,XX testis-DSD (T-DSD) in pigs. Clinical and transcriptome sequencing analyses were performed on DSD and normal female pigs. Cytogenetic and SRY analyses confirmed that OT/T-DSD pigs exhibited a 38,XX karyotype and lacked the SRY gene. The DSD pigs had higher levels of follicle-stimulating hormone, luteinizing hormone, and progesterone, but lower testosterone levels when compared with normal male pigs. The reproductive organs of OT/T-DSD pigs exhibit abnormal development, displaying both male and female characteristics, with an absence of germ cells in the seminiferous tubules. Sex determination and development-related differentially-expressed genes (DEGs) shared between DSD pigs were identified in the gonads, including WT1, DKK1, CTNNB1, WTN9B, SHOC, PTPN11, NRG1 and NXK3–1. DKK1 is proposed as a candidate gene for investigating the regulatory mechanisms underlying gonadal phenotypic differences between OT-DSD and T-DSD pigs. Consequently, our findings provide insights into the molecular pathogenesis of DSD pigs and present an animal model for studying into DSD in humans.

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Rat Stem-Cell Leukemia Gene Expression Increased during Testis Maturation

Cited by 3 publications

References 36 publications

Histological and transcriptome analyses of testes from Duroc and Meishan boars

Histological and transcriptome analyses of testes from Duroc and Meishan boars

Tumor Suppressor Candidate TUSC3 Expression during Rat Testis Maturation

Clinical and gonadal transcriptome analysis of 38,XX disorder of sex development pigs

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