Expression of RAD51 and Its Clinical Impact in Oral Squamous Cell Carcinoma

Abstract

Purpose. To examine the expression of RAD51 in oral squamous cell carcinoma (OSCC) and analyze its connection with pathological grade, clinical stage, and lymphatic metastasis potential. Methods. For this study, 74 OSCC samples, 15 normal mucosa tissues, and 11 normal skin tissue samples were collected. RAD51 expression was investigated using immunohistochemistry. A follow-up visit was used to assess the prognosis of each patient. We compared RAD51 expression in oral mucosa epithelial cells (OMECs), keratinocytes, and tongue squamous cell carcinoma cells (TSCCs) by Western blot analysis. Results. RAD51 expression was higher in tumor cells than in normal mucosal tissues. In addition, RAD51 expression was associated with higher tumor differentiation (P<0.05). Also, RAD51 expression was higher (P<0.05) in patients with lymphatic metastases, and relapse rates were also higher in patients with elevated RAD51 levels (P=0.052). In addition, RAD51 expression levels were highest in the skin keratinocytes, followed by the TSCCs and OMECs. Conclusion. A strong positive correlation was found between RAD51 expression and the degree of malignancy in OSCC patients, suggesting that RAD51 could be an excellent prognostic indicator for OSCC patients.

DNA Double-Strand Break Response and Repair Gene Polymorphisms May Influence Therapy Results and Prognosis in Head and Neck Cancer Patients

“…RAD51 overexpression has also been shown to lead to the transcriptional activation of pro-metastatic genes and the promotion of cancer progression [60]. Elevated RAD51 levels were found in oral cancer, especially in patients with lymph node metastases, and indicated a poor prognosis [16]. The RAD51 rs1801321 -61G>T (alias -172G>T) in the 5 UTR was demonstrated to enhance promoter activity [24].…”

“…In HNSCC, for example, NBS1/NBN and XRCC5 overexpression was associated with metastasis and locoregional failure after RT [13,14]. In oral cancer, high levels of MRE11 and RAD51 correlated with radiation resistance and poor prognosis [15,16]. Significant inter-individual differences in response to RT and CT are observed in HNC patients which may be due to, among others, host genetic factors such as SNPs.…”

Screening for Biomarkers for Progression from Oral Leukoplakia to Oral Squamous Cell Carcinoma and Evaluation of Diagnostic Efficacy by Multiple Machine Learning Algorithms

“…A gradual increase in CDC6 was observed in the normal mucosa, OL, and OSCC tissues, which is consistent with our findings. Yuyang Li [ 35 ] reported that the RAD51 expression was higher in OSCC compared to normal mucosal tissues. Yasuyuki Minakawa [ 36 ] and Yun Zhang [ 37 ] showed that KIF4A promotes the proliferation of OSCC cell lines.…”

Scientific Research Directions on the Histopathology and Immunohistochemistry of the Cutaneous Squamous Cell Carcinoma: A Scientometric Study

“…For assessing the impact of and to establish the research directions for squamous cell carcinomas, we used the scientometric method [ 3 , 4 ]. A set of instructions was defined based on the keywords from the 76 articles we assessed [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , …”