Expression of PTEN protein in patients with laryngeal squamous cell carcinoma

Güney, Kenan; Özbilim, Gülay; Derin, Alper Tunga; Çetin, Safiye

doi:10.1016/j.anl.2007.03.014

Cited by 16 publications

(11 citation statements)

References 19 publications

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“…Squarize et al [31] found a negative relationship between PTEN expression and the grade in oral squamous cell carcinomas and reported that PTEN was an independent prognostic factor. In a study reporting that there was a decrease in PTEN expression in laryngeal tumors, there was no relationship between PTEN expression and tumor localization, tumor size, differentiation and stage [32]. We could not find a correlation between PTEN expression and tumor size, differentiation, lymph node metastasis and invasion depth.…”

Section: Discussioncontrasting

confidence: 63%

Poor prognostic clinicopathologic features correlate with VEGF expression but not with PTEN expression in squamous cell carcinoma of the larynx

Süllü

Gün²,

Atmaca

et al. 2010

Diagn Pathol

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BackgroundThe aim of this study was to assess the relationship between expression of vascular endothelial growth factor (VEGF) and phosphatase and tensin homolog deleted in chromosome ten (PTEN), angiogenesis and clinicopathological parameters of squamous cell carcinoma of the larynx.MethodsWe examined immunohistochemical expression of VEGF and PTEN and CD34 for microvessel density (MVD) in sections of formalin-fixed, paraffin embedded tissue blocks of 140 patients with squamous cell carcinoma of the larynx. The intensity of VEGF and PTEN staining and the proportion of cells staining were scored.ResultsThe tumor grade was not significantly related to PTEN expression, but it was to VEGF expression (p = 0.400; p = 0.015, respectively). While there was no significant relationship between PTEN expression and tumor size and cartilage invasion (p = 0.311, p = 0.128), there was a significant relationship between the severity of VEGF expression and tumor size (p = 0.006) and lymph node metastasis (p = 0.048) but not cartilage invasion (p = 0.129). MVD was significantly higher in high-grade tumors (p = 0.003) but had no significant relationship between MVD, lymph node metastasis, and cartilage invasion (p = 0.815, p = 0.204). There was also no significant relationship between PTEN and VEGF expression (p = 0.161) and between PTEN and VEGF expression and the MVD (p = 0.120 and p = 0.175, respectively).ConclusionsIncreased VEGF expression may play an important role in the outcome of squamous cell carcinoma of the larynx. PTEN expression was not related to VEGF expression and clinicopathological features of squamous cell carcinoma of the larynx.

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Section: Discussioncontrasting

confidence: 63%

Poor prognostic clinicopathologic features correlate with VEGF expression but not with PTEN expression in squamous cell carcinoma of the larynx

Süllü

Gün²,

Atmaca

et al. 2010

Diagn Pathol

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“…In numerous cancer cell lines and primary tumours, mutations in the PTEN gene and loss of heterozygosity or homozygous deletions have been found (Cairns et al, 1997;Guldberg et al, 1997;Guney et al, 2007). As described earlier, PTEN regulates AKT activity through dephosphorylation of PIP3 (Sarbassov et al, 2005).…”

Section: Discussionmentioning

confidence: 95%

“…The PTEN/MMAC1 was originally described as a tumour suppressor in a large variety of malignant tumours (Li et al, 1997;Panigrahi et al, 2004;Guney et al, 2007). In numerous cancer cell lines and primary tumours, mutations in the PTEN gene and loss of heterozygosity or homozygous deletions have been found (Cairns et al, 1997;Guldberg et al, 1997;Guney et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The phosphatase and tensin homologue deleted on chromosome 10 mediates radiosensitivity in head and neck cancer

et al. 2010

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BACKGROUND: For locally advanced squamous cell carcinoma of the head and neck (HNSCC), the recurrence rate after surgery and postoperative radiotherapy is between 20 and 40%, and the 5-year overall survival rate is B50%. Presently, no markers exist to accurately predict treatment outcome. Expression of proteins in the human epidermal growth factor receptor (EGFR) pathway has been reported as a prognostic marker in several types of cancer. METHODS: The aim of this study was to investigate the prognostic value of proteins in the EGFR pathway in HNSCC. For this purpose, we collected surgically resected tissue of 140 locally advanced head and neck cancer patients, all treated with surgery and postoperative radiotherapy. RESULTS: In a multivariate analysis, expression of the phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was significantly related to worse locoregional control (LRC; HR: 2.2, 95% CI: 1.1 -4.6; P ¼ 0.03), independent of lymph node metastases (HR: 5.6, 95% CI: 1.2 -27.4; P ¼ 0.03) and extranodal spread (HR: 2.7; 95% CI: 1.2 -6.5; P ¼ 0.02). In vitro clonogenic radiosensitivity assays confirmed that overexpression of PTEN resulted in increased radioresistance. CONCLUSION: Our study is the first report showing that expression of PTEN mediates radiosensitivity in vitro and that increased expression in advanced HNSCC predicts worse LRC.

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“…Data in the literature on the association of PTEN with clinicopathologic parameters and LNM are highly controversial. One previous study (Sullu et al, 2010) found no significant relationship between PTEN expression and tumor size, differentiation, depth of invasion, and LNM, and in a study reporting that there was a decrease in PTEN expression in laryngeal tumors, there was no relationship between PTEN expression and tumor localization, tumor size, differentiation, and stage (Guney et al, 2007). Also, our study, in spite of documenting an association between PTEN expression and invasion of both the cartilage and the muscle, revealed no significant association between PTEN and E-cadherin expression, although PTEN has been previously shown to interact with cell adhesion complexes and to stabilize intercellular junctions (Kotelevets et al, 2001).…”

Section: Discussionmentioning

confidence: 88%

Immunohistochemical expression of epidermal growth factor receptor, PTEN, and E-cadherin in laryngeal squamous cell carcinoma

El-Gendi¹,

Kazem²

2011

Egyptian Journal of Pathology

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Background The prognostic stratification of laryngeal squamous cell carcinoma (LSCC) patients is inadequate as patients affected by tumors with similar clinicopathological parameters, and undergoing similar treatment, may differ in prognosis. The current study was undertaken to assess the immunohistochemical expression of epidermal growth factor receptor (EGFR), E-cadherin, and phosphatase and tensin homolog deleted in chromosome ten (PTEN) in 52 retrospective LSCC cases. We also investigated the prognostic implication of deregulated marker expression in relation to the clinicopathological features of LSCC, especially neck nodal metastasis.Results Decreased histologic differentiation, supraglottic tumor location, low or negative EGFR, and low or negative E-cadherin expression were significant predictors of lymph node metastasis (LNM) (P < 0.001, P = 0.011, P < 0.001, and P = 0.033, respectively), whereas patients' age, increased T-stage, cartilage invasion, muscle invasion, and differences in PTEN expression were not predictors of LNM. A receiver operator characteristic curve analysis showed that the predictability of EGFR and E-cadherin expression in discriminating LNM-positive cases was good and poor, respectively. Three models were developed using only the variables that revealed significant association with LNM in bivariate analysis and we tested their predictability to discriminate LNM-positive from LNM-negative cases. All models were significantly better than the basic model containing the constant only (P < 0.001 for the three models). A receiver operator characteristic analysis was performed, and it revealed that the predictive power of the first (tumor grade and site) and second (tumor grade, site, and EGFR) models ranged from good to excellent, whereas that of the third model (tumor grade, site, EGFR, and E-cadherin) was excellent. The number of correctly diagnosed cases (overall accuracy) increased only in the second compared with the first model, but the third model had an overall accuracy equal to that of the second model.Conclusion Nodal metastasis can be predicted by combining tumor grade and site in an equation, and if an immunohistochemical marker is to be added to increase the ability to identify patients with clinically negative nodes, our results suggest it to be EGFR. Although E-cadherin did not increase LNM predictability when added, this may be due to the limitation of the sample size. Thus, according to our results, we recommend an evaluation of these promising molecular markers on a larger sample size in future studies.

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Expression of PTEN protein in patients with laryngeal squamous cell carcinoma

Cited by 16 publications

References 19 publications

Poor prognostic clinicopathologic features correlate with VEGF expression but not with PTEN expression in squamous cell carcinoma of the larynx

Poor prognostic clinicopathologic features correlate with VEGF expression but not with PTEN expression in squamous cell carcinoma of the larynx

The phosphatase and tensin homologue deleted on chromosome 10 mediates radiosensitivity in head and neck cancer

Immunohistochemical expression of epidermal growth factor receptor, PTEN, and E-cadherin in laryngeal squamous cell carcinoma

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