Expression of Protein Kinases C βI, βII, and VEGF during the Differentiation of Enamel Epithelium in Tooth Development

Aida, Mina; Irié, Tarou; Aida, Tadateru; Tachikawa, Tetsuhiko

doi:10.1177/154405910508400305

Cited by 22 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Amelogenin was used as a marker of inner enamel epithelium differentiation in ameloblasts (Aida et al 2005;Suzawa et al 2006), and DSPP was used as a marker for dental papilla cell differentiation in odontoblasts (Saito et al 2004;Deng et al 2005).…”

Section: Real-time Polymerase Chain Reactionmentioning

confidence: 99%

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts

et al. 2010

View full text Add to dashboard Cite

Morphogenesis and cytodifferentiation are distinct processes in tooth development. Cell proliferation predominates in morphogenesis; differentiation involves changes in form and gene expression. The cytoskeleton is essential for both processes, being regulated by Rho GTPases. The aim of this study was to verify the expression, distribution, and role of Rho GTPases in ameloblasts and odontoblasts during tooth development in correlation with actin and tubulin arrangements and amelogenin and dentin sialophosphoprotein (DSPP) expression. RhoA, Rac1, and Cdc42 were strongly expressed during morphogenesis; during cytodifferentiation, RhoA was present in ameloblasts and odontoblasts, Rac1 and its effector Pak3 were observed in ameloblasts; and Cdc42 was present in all cells of the tooth germ and mesenchyme. The expression of RhoA mRNA and its effectors RockI and RockII, Rac1 and Pak3, as analyzed by real-time polymerase chain reaction, increased after ameloblast and odontoblast differentiation, according to the mRNA expression of amelogenin and DSPP. The inhibition of all Rho GTPases by Clostridium difficile toxin A completely abolished amelogenin and DSPP expression in tooth germs cultured in anterior eye chamber, whereas the specific inhibition of the Rocks showed only a partial effect. Thus, both GTPases are important during tooth morphogenesis. During cytodifferentiation, Rho proteins are essential for the complete differentiation of ameloblasts and odontoblasts by regulating the expression of amelogenin and DSPP. RhoA and its effector RockI contribute to this role. A specific function for Rac1 in ameloblasts remains to be elucidated; its punctate distribution indicates its possible role in exocytosis/endocytosis.

show abstract

Section: Real-time Polymerase Chain Reactionmentioning

confidence: 99%

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts

et al. 2010

View full text Add to dashboard Cite

show abstract

“…1D). In the developing tooth, VEGF and VEGFR-2 are expressed in odontoblasts and the inner enamel epithelium may regulate odontoblast development and the differentiation of inner enamel epithelium to ameloblasts (Aida et al, 2005;Miwa et al, 2008) VEGF expression in pulp fibroblasts and odontoblasts of human teeth is higher in immature than mature permanent teeth, suggesting a role of VEGF in tooth maturation (Wang et al, 2007). In the enamel knot cells that were visible in a developing tooth germ during the late cap stage, the expression was weaker than in the control group.…”

Section: Resultsmentioning

confidence: 99%

The Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Tooth Development in Rats

Koparal¹,

Er²,

Deveci³

et al. 2015

Int. J. Morphol.

View full text Add to dashboard Cite

SUMMARY: Dithiocarbamate propinebs are organometal fungicides that are widely used for the control of diseases in plants. In this study, pregnant female rats received 400 ppm propineb concentrations in 5 ml distilled water for 16 days of gestation, and then infant rats were obtained by cesarean section. In the histological analysis on the frontal sections, the use of propineb was found effective on odontoblast cell hyperplasia, cell infiltration in the dental papilla, and degeneration in the mesenchymal cells of the outer enamel. The expression of MMP-2 (Matrix Metalloproteinase-2) and VEGF (Endothelial cell growth factor) in the connective tissue was evaluated by immunohistochemistry. The drinking water given to the mothers in propineb tooth bud, enamel and dentin, resulted in morphological changes suggestive of a delay in formation, which cross the placental barrier and possibly affect the tooth development.

show abstract

“…Among the factors involved in the new vessel formation, VEGF-A and FGF-2 are two of the factors necessary to initiate angiogenesis and recruit endothelial cells [73]. VEGF-A and FGF-2 are present in and regulate the formation of enamel and dentin [74,75]. However, the effects of amelogenin on angiogenesis or in the regulation of these growth factors are unclear.…”

Section: Discussionmentioning

confidence: 99%

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

et al. 2013

View full text Add to dashboard Cite

Enamel matrix derivative (EMD), a decellularized porcine extracellular matrix (ECM), is used clinically in periodontal tissue regeneration. Amelogenin, EMD's principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases proteolytically cleaved peptides. However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. Human MG63 osteoblast-like cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5 kDa tyrosine-rich amelogenin peptide (TRAP) isolated from EMD and the effect on osteogenesis, local factor production, and apoptosis assessed. Treated MG63 cells increased alkaline phosphatase specific activity and levels of osteocalcin, osteoprotegerin, prostaglandin E 2 , and active/latent TGF-1, an effect sensitive to the effector and concentration. Primary osteoblasts exhibited similar, but less robust, effects. TRAP-rich 5 kDa peptides yielded more mineralization than rhAmelogenin in osteoblasts in vitro. Both amelogenin and 5 kDa peptides protected MG63s from chelerythrine-induced apoptosis. The data suggest that the 5 kDa TRAP-rich sequence is an active amelogenin peptide that regulates osteoblast differentiation and local factor production and prevents osteoblast apoptosis.

show abstract

Expression of Protein Kinases C βI, βII, and VEGF during the Differentiation of Enamel Epithelium in Tooth Development

Cited by 22 publications

References 19 publications

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts

The Effects of Polymeric Zinc Propylen-Bis-Dithiocarbamate (Propineb) on Tooth Development in Rats

Amelogenin Peptide Extract Increases Differentiation and Angiogenic and Local Factor Production and Inhibits Apoptosis in Human Osteoblasts

Contact Info

Product

Resources

About