Expression of Protein Kinase C Isozymes That Are Required for Chondrogenesis of Chick Limb Bud Mesenchymal Cells

Choi, Bohm; Chun, Jang‐Soo; Lee, Young-Sup; Sonn, Jong-Kyung; Kang, Shin‐Sung

doi:10.1006/bbrc.1995.2724

Cited by 45 publications

(30 citation statements)

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“…We previously showed that Erk and p38 MAPK regulate mesenchymal cell chondrogenesis by modulating the expression of various cell adhesion molecules, including N-cadherin, fibronectin, and integrins (Choi et al, 1995;Oh et al, 2000). Here, we observed that the phosphorylation level of Erk decreased as the diameter of the Ti-nanotubes on the culture substrates increased ( Figure 4A).…”

Section: Erk Signaling Is Involved In the Apparent Chondrocytic Diffesupporting

confidence: 56%

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

et al. 2011

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Vertically aligned, laterally spaced nanoscale titanium nanotubes were grown on a titanium surface by anodization, and the growth of chondroprogenitors on the resulting surfaces was investigated. Surfaces bearing nanotubes of 70 to 100 nm in diameter were found to trigger the morphological transition to a cortical actin pattern and rounded cell shape (both indicative of chondrocytic differentiation), as well as the up-regulation of type II collagen and integrin β4 protein expression through the down-regulation of Erk activity. Inhibition of Erk signaling reduced stress fiber formation and induced the transition to the cortical actin pattern in cells cultured on 30-nm-diameter nanotubes, which maintained their fibroblastoid morphologies in the absence of Erk inhibition. Collectively, these results indicate that a titanium-based nanotube surface can support chondrocytic functions among chondroprogenitors, and may therefore be useful for future cartilaginous applications.

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Section: Erk Signaling Is Involved In the Apparent Chondrocytic Diffesupporting

confidence: 56%

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

et al. 2011

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“…Previously, our laboratory showed that chondrogenic differentiation of chick limb bud mesenchymal cells is regulated by complex protein kinase signaling cascades involving PKC, MAPKs and Akt (Choi et al, 1995;Chang et al, 1998;Oh et al, 1999;Yoon et al, 2000). Curcumin has been shown to inhibit tyrosine kinase activity in AU565 tumor cells (Narasimhan and Ammanamanchi, 2008).…”

Section: Discussionmentioning

confidence: 99%

Curcumin inhibits cellular condensation and alters microfilament organization during chondrogenic differentiation of limb bud mesenchymal cells

Kim¹,

Kim

Kang

et al. 2009

Exp Mol Med

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Curcumin is a well known natural polyphenol product isolated from the rhizome of the plant Curcuma longa, anti-inflammatory agent for arthritis by inhibiting synthesis of inflammatory prostaglandins. However, the mechanisms by which curcumin regulates the functions of chondroprogenitor, such as proliferation, precartilage condensation, cytoskeletal organization or overall chondrogenic behavior, are largely unknown. In the present report, we investigated the effects and signaling mechanism of curcumin on the regulation of

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“…PKC enzyme activity proved to be detectable in cells of HDC with characteristic changes during differentiation, and conversely, exposure of cultures to long-term phorbol-12-myristate-13-acetate (PMA) treatments blocked the differentiation process and abolished in vitro cartilage matrix production [25]. In a study conducted by Choi and his colleagues the expression profiles of classic (PKCalpha and gamma), novel (PKCepsilon) and atypical (PKCzeta, lambda and iota) protein kinase C isoenzymes have been described during the differentiation of chicken micromass cultures and they showed that expression of various cPKC and nPKC isoforms is mostly required at the early stages of in vitro cartilage formation [3]. However, Choi and his group failed to detect PKCdelta expression in cells of HDC with the antibodies employed [3].…”

Section: Discussionmentioning

confidence: 99%

“…In a study conducted by Choi and his colleagues the expression profiles of classic (PKCalpha and gamma), novel (PKCepsilon) and atypical (PKCzeta, lambda and iota) protein kinase C isoenzymes have been described during the differentiation of chicken micromass cultures and they showed that expression of various cPKC and nPKC isoforms is mostly required at the early stages of in vitro cartilage formation [3]. However, Choi and his group failed to detect PKCdelta expression in cells of HDC with the antibodies employed [3]. It has also been reported that protein kinase A signalling regulates in vitro chondrogenesis of chicken mesenchymal cells via the PKCalpha pathway [26].…”

Section: Discussionmentioning

confidence: 99%

“…As a result, chondrogenic mesenchymal cells become closely packed, which initiates a complex and a yet not fully understood signalling mechanism that governs differentiation of cells within these condensations into chondroblasts and then mature chondrocytes [1]. Among a number of signalling molecules, members of the phospholipid-dependent serine/threonine protein kinase C (PKC) family are known regulators of in vitro chondrogenesis [2,3].…”

Section: Introductionmentioning

confidence: 99%

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PKCdelta is a positive regulator of chondrogenesis in chicken high density micromass cell cultures

et al. 2011

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a b s t r a c tWe aimed to elucidate the role of the Ca-independent PKC isoenzyme PKCdelta in the regulation of spontaneous in vitro chondrogenesis occurring in a 6-day-long culturing period in chicken limb budderived high density cell cultures (HDC). PKCdelta expression and activity were detectable throughout the entire culturing period with a peak on days 2 and 3, when most of the chondroblasts differentiate. To inhibit the activity of PKCdelta, either the natural compound rottlerin was transiently applied to the culture medium of HDC in 2.5, 5 or 10 mM concentrations, or gene silencing was performed by using PKCdelta shRNA. Rottlerin significantly reduced the overall PKC activity in enzyme activity assays of cell-free samples of untreated control HDC, probably via the inhibition of PKCdelta. On the contrary, we were unable to detect any consistent change of PKC enzyme activity assayed in samples of HDC treated with rottlerin during culturing. PKCdelta gene silencing resulted in a significantly lower PKC activity. Both rottlerin and PKCdelta shRNA caused a severe reduction in cartilage formation, further more protein and phospho-protein levels of Sox9, the key transcription factor of chondrogenesis, were also significantly decreased. Rottlerin lowered, while PKCdelta gene silencing elevated the phosphorylation status of ERK1/2. Our data suggest that PKCdelta stimulates chondrogenesis via influencing Sox9 and ERK1/2 phosphorylation, but the inhibition of cartilage formation in the rottlerin-treated HDC is probably PKCdelta independent and rottlerin might have different effects when applied to cells or to an in vitro enzyme activity assay.

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Expression of Protein Kinase C Isozymes That Are Required for Chondrogenesis of Chick Limb Bud Mesenchymal Cells

Cited by 45 publications

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TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

Curcumin inhibits cellular condensation and alters microfilament organization during chondrogenic differentiation of limb bud mesenchymal cells

PKCdelta is a positive regulator of chondrogenesis in chicken high density micromass cell cultures

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Expression of Protein Kinase C Isozymes That Are Required for Chondrogenesis of Chick Limb Bud Mesenchymal Cells

Cited by 45 publications

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TiO2nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO2nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

Curcumin inhibits cellular condensation and alters microfilament organization during chondrogenic differentiation of limb bud mesenchymal cells

PKCdelta is a positive regulator of chondrogenesis in chicken high density micromass cell cultures

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TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity

TiO₂nanotube stimulate chondrogenic differentiation of limb mesenchymal cells by modulating focal activity