Expression of progesterone receptor protein in the ovine uterus during the estrous cycle: Effects of nutrition, arginine and FSH

Grazul-Bilska, Anna T.; Thammasiri, Jiratti; Kraisoon, Aree; Reyaz, Arshi; Bass, Casie S.; Kaminski, Samantha L.; Navanukraw, Chainarong; Redmer, Dale A.

doi:10.1016/j.theriogenology.2017.11.008

Cited by 13 publications

(6 citation statements)

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“…Furthermore, we have detected expression of steroid receptors in EBV and MBV, and expression of mRNA for ESR1 in MBV, ESR2 in EBV and MBV differed among days of pregnancy. Similar to our study, estrogens and P4 steroid receptors have been detected in uterine and placental blood vessels of several species [9,28,31–35, 36]. Changes in ESR mRNA expression in blood vessels during early pregnancy are likely associated with the regulatory role estrogens play in vascular functions in the uterus and placenta such as regulation of blood flow, vascular tone, promotion of angiogenesis, blood vessel remodeling and others [32,37–40].…”

Section: Discussionsupporting

confidence: 85%

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Grazul-Bilska

Bairagi

Kraisoon

et al. 2019

Domestic Animal Endocrinology

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Sex steroid hormones are major regulators of uterine and placental growth and functions, as well as many other biological processes. To examine the mRNA expression of nuclear estrogen (ESR1 and 2) and progesterone (PGRAB and B) receptors in different compartments of the uterus and placenta, tissues were collected in experiment 1, on days 16, 20 and 28 after natural mating (NAT) and on day 10 after estrus (non-pregnant controls [NP]); and in experiment 2, on day 22 of NAT, and pregnancies established after transfer of embryos generated through mating of FSH-treated ewes (NAT-ET), in vitro fertilization (IVF), or in vitro activation (IVA; parthenotes). In experiment 1, ESR1 expression in endometrial stroma (ES), endometrial glands (EG) and myometrial blood vessels (MBV), ESR2 in endometrial blood vessels (EBV), PGRAB in ES, and PGRB in ES, EG and MBV was greater in pregnant than NP depending on day of pregnancy. Day of pregnancy affected expression of ESR1 in MBV, ESR2 in EBV and MBV, and PGRAB in ES. In experiment 2, ESR1, PGRAB and PGRB in EG, but not in other compartments, was greater in NAT-ET than NAT, and PGRB was greater in NAT-ET than the IVF. These data demonstrated that the ESR and PGR expression was different in pregnant vs. NP ewes in selected compartments, and was affected by pregnancy stage or embryo origin in selected utero-placental compartments. Thus, sex steroid hormone mRNA expression is differentially regulated in a spatio-temporal manner in uterus and placenta, and is affected by application of assisted reproductive technology in sheep.

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Section: Discussionsupporting

confidence: 85%

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Grazul-Bilska

Bairagi

Kraisoon

et al. 2019

Domestic Animal Endocrinology

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“…The spatio-temporal expression of PGR and ESR in uterine and utero-placental tissues, and in fetal membranes (FM) has been demonstrated for several species such as mice, cows, horses, pigs, sheep and primates from early to late pregnancy [19-22]. We have recently demonstrated that mRNA expression of PGR and ESR changes in ovine utero-placental tissues during early pregnancy [1].…”

Section: Introductionmentioning

confidence: 99%

Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression

et al. 2018

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The aim of this study was to evaluate the pattern of protein expression of the steroid receptor isoforms of nuclear progesterone receptors (PGR) A and B, and estrogen receptors (ESR1 and 2) in utero-placental compartments during early pregnancy. Utero-placental tissues were collected from days 14-30 (n = 4 ewes/day), and uterine tissues were collected from non-pregnant ewes on day 10 after estrus (n = 4). Cross sections of formalin-fixed and paraffin embedded tissues were immunofluorescently stained to detect PGRAB, PGRB, ESR1 and ESR2, followed by image generation of entire cross-sections of uterine and utero-placental tissues, confocal imaging of individual uterine and utero-placental compartments, and image and statistical analyses. PGRAB, PGRB, ESR1 and ESR2 were detected in several compartments of uterine and utero-placental tissues. Quantitative image analysis of staining intensity demonstrated that compared to non-pregnant controls 1) expression of PGRAB and PGRB was less in luminal epithelium and endometrial glands from day 14-16 till 30; 2) PGRAB expression tended to be greater in endometrial and myometrial blood vessels on days 28 and/or 30; 3) PGRB expression in myometrum was lower on days 16 and 28; 4) ESR1 in endometrial stroma was lower in all days of pregnancy; 5) ESR2 expression was similar in all compartments and not affected by pregnancy stage; and 6) in FM, expression of steroid receptors was similar. Thus, we have demonstrated spatial and temporal expression of nuclear PGR and ESR isoforms in utero-placental compartments during early pregnancy.

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“…Immunohistochemistry was performed as described previously (Bairagi et al, 2018; Grazul‐Bilska, Thammasiri, et al, 2018). Paraffin‐embedded tissue samples were cut into 5 μm thick, mounted onto positively charged glass slides, de‐paraffinized, and rehydrated in xylene and graded series of ethanol.…”

Section: Methodsmentioning

confidence: 99%

Effects of FSH treatment and withdrawal during proestrus on uterine proliferation and steroid hormone receptor expression in beef heifers

Bunma

Kanjanaruch

Kogram

et al. 2021

Animal Science Journal

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To determine the effects of Follicle Stimulating Hormone (FSH) treatment and subsequent withdrawal on uterine proliferation and estrogen receptor (ESR), Brahman crossbred heifers (n = 12) were twice daily injected with FSH (4, 3, and 2 mg/injection) on Days 17–19 of the estrous cycle (FSH 3 days) and (4 and 3 mg/injection) on Days 17–18 (FSH 2 days) and withdrawal with saline on Day 19 and (4 mg/injection) on Day 17 (FSH 1 day) and withdrawal with saline on Days 18–19. Uterine tissue was subjectively collected on Day 20 and microscopically classified to four regions: endometrial stroma (ES), surface endometrial gland (EG), deep endometrial gland (DG), and myometrium (Myo). The cell proliferation marker, Ki‐67, was quantified as labeling index (LI) in uterine regions, and tissues were immunostained to detect ESR2 followed by image analysis. The LI of ES, EG, and DG was greater (P = 0.0018, P = 0.0005, and P = 0.0103; respectively) in heifers received FSH for 3 days. The expression of ESR2 protein on ES and EG was greatest (P < 0.0001 and P = 0.0036, respectively) in FSH 3 days‐treated group. Thus, FSH administration during proestrus stimulates uterine cell proliferation, and ESR2 expressions are affected by FSH during proestrus and differentially distributed in the uterine regions.

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Expression of progesterone receptor protein in the ovine uterus during the estrous cycle: Effects of nutrition, arginine and FSH

Cited by 13 publications

References 62 publications

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression

Effects of FSH treatment and withdrawal during proestrus on uterine proliferation and steroid hormone receptor expression in beef heifers

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