Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression

Bairagi, Soumi; Grazul-Bilska, Anna T.; Borowicz, P. P.; Reyaz, Arshi; Valkov, Veselina; Reynolds, Lawrence P.

doi:10.1016/j.theriogenology.2018.04.002

Cited by 20 publications

(20 citation statements)

References 67 publications

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“…A loss of PR has been suggested to support embryo development and implantation [9]. In cattle, the loss of the PR protein in the LE and GE is observed from day 12/13 of the cycle and during pregnancy [5,6], whereas in pregnant sheep, this takes place from day 14 onward [7]. This might indicate that the loss of the LE PR in the roe deer takes place before diapause or is associated with the induction of diapause.…”

Section: Discussionmentioning

confidence: 99%

“…During preimplantation embryo development, ovarian progesterone (P4) stimulates the secretory function of the endometrial LE and GE. Although the P4 concentration remains high during the luteal phase and early pregnancy in ruminants [5][6][7], the LE and GE gradually loose the expression of the progesterone receptor (PR) from days 12 to 14 onward during both the normal cycle and pregnancy [5][6][7]. Upon loss of the PR in the LE and GE, P4 is known to act indirectly via PR expressed on stromal cells and the induction of progestamedins [8].…”

Section: Introductionmentioning

confidence: 99%

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Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau†

Weijden

Puntar

Vegas

et al. 2019

Biology of Reproduction

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Numerous intrauterine changes take place across species during embryo development. Following fertilization in July/August, the European roe deer (Capreolus capreolus) embryo undergoes diapause until embryonic elongation in December/January. Embryonic elongation prior to implantation is a common feature among ungulates. Unlike many other ruminants, the roe deer embryo does not secrete interferon-tau (IFNτ). This provides the unique opportunity to unravel IFNτ-independent signaling pathways associated with maternal recognition of pregnancy (MRP). This study aimed at identifying the cell-type–specific endometrial gene expression changes associated with the MRP at the time of embryo elongation that are independent of IFNτ in roe deer. The messenger RNA (mRNA) expression of genes known to be involved in embryo–maternal communication in cattle, pig, sheep, and mice was analyzed in laser capture microdissected (LMD) endometrial luminal, glandular epithelial, as well as stromal cells. The mRNA transcript abundances of the estrogen (ESR1), progesterone receptor (PGR), and IFNτ-stimulated genes were lower in the luminal epithelium in the presence of an elongated embryo compared to diapause. Retinol Binding Protein-4 (RBP4), a key factor involved in placentation, was more abundant in the luminal epithelium in the presence of an elongated embryo. The progesterone receptor localization was visualized by immunohistochemistry, showing an absence in the luminal epithelium and an overall lower abundance with time and thus prolonged progesterone exposure. Our data show a developmental stage-specific mRNA expression pattern in the luminal epithelium, indicating that these cells sense the presence of an elongated embryo in an IFNτ-independent manner.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau†

Weijden

Puntar

Vegas

et al. 2019

Biology of Reproduction

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“…Several processes which are critical for pregnancy establishment take place during early pregnancy including implantation, placentation, and initiation of fetal and placental growth [1–3]. We have recently demonstrated that expression of steroid hormone receptors and factors involved in the regulation of growth and angiogenesis change dramatically during early pregnancy, and are affected by pregnancy stage and embryo origin [4–9].…”

Section: Introductionmentioning

confidence: 99%

“…Steroid hormones exert their direct effects through nuclear and membrane receptors [10–12]. Uterine and placental expression of estrogen and progesterone receptors (ER and PR, respectively) has been well described for numerous species [3,8,9,10–14]. Steroid receptors are expressed in several uterine and placental compartments, and their expression is regulated by estradiol-17β (E2), progesterone (P4) and other regulatory factors [11].…”

Section: Introductionmentioning

confidence: 99%

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Grazul-Bilska

Bairagi

Kraisoon

et al. 2019

Domestic Animal Endocrinology

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Sex steroid hormones are major regulators of uterine and placental growth and functions, as well as many other biological processes. To examine the mRNA expression of nuclear estrogen (ESR1 and 2) and progesterone (PGRAB and B) receptors in different compartments of the uterus and placenta, tissues were collected in experiment 1, on days 16, 20 and 28 after natural mating (NAT) and on day 10 after estrus (non-pregnant controls [NP]); and in experiment 2, on day 22 of NAT, and pregnancies established after transfer of embryos generated through mating of FSH-treated ewes (NAT-ET), in vitro fertilization (IVF), or in vitro activation (IVA; parthenotes). In experiment 1, ESR1 expression in endometrial stroma (ES), endometrial glands (EG) and myometrial blood vessels (MBV), ESR2 in endometrial blood vessels (EBV), PGRAB in ES, and PGRB in ES, EG and MBV was greater in pregnant than NP depending on day of pregnancy. Day of pregnancy affected expression of ESR1 in MBV, ESR2 in EBV and MBV, and PGRAB in ES. In experiment 2, ESR1, PGRAB and PGRB in EG, but not in other compartments, was greater in NAT-ET than NAT, and PGRB was greater in NAT-ET than the IVF. These data demonstrated that the ESR and PGR expression was different in pregnant vs. NP ewes in selected compartments, and was affected by pregnancy stage or embryo origin in selected utero-placental compartments. Thus, sex steroid hormone mRNA expression is differentially regulated in a spatio-temporal manner in uterus and placenta, and is affected by application of assisted reproductive technology in sheep.

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“…In general, progesterone blocks the sexual behavior induced by estradiol in female sheep [14] . Estrogen and progesterone regulate the uterine endocrine and paracrine signals through their receptors during the estrus cycle and pregnancy in sheep [15,16] . There was no study on PMSG-estrus induction during estrus interval after lambing in spring in Small-tail Han sheep.…”

Section: Introductionmentioning

confidence: 99%

Koyunlarda Östrus İndüksiyonu Sonrası Ovaryum, Oviduct ve Endometriyumda Folikül Uyarıcı Hormon, Luteotropik Hormon, Östrojen ve Progesteron Reseptör Ekspresyonu

Yang¹,

Xiao-lei²,

Chen³

et al. 2019

Kafkas Univ Vet Fak Derg

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Induction of estrus is an effective management tool for increasing the pregnancy rate in ewes, and there is a long estrus interval after lambing in spring in Small-tail Han ewe. In this study, norgestrel releasing intra-vaginal devices (PRID) and PRID + pregnant mare serum gonadotrophin (PMSG) were used for estrus induction in Small-tail Han ewe, and expression of follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), estrogen receptor (ER) and progesterone receptor (PGR) mRNA in the ovarial stroma, oviduct ampulla and endometrium was analyzed by quantitative real-time PCR. The results showed that the estrus-induction with PRID and PRID + PMSG almost had the same effects on increasing the percentage of animals in estrus and conception rate of the ewes, and the estrus-induction had no significant effect on the expression of LHR, ER and PGR mRNA in the oviduct ampulla, ovarial stroma and endometrium, and FSHR in the ovarial stroma and endometrium. Furthermore, the estrus-induction could significantly decrease the expression of FSHR mRNA in the ovine oviduct ampulla (P<0.05). Therefore, it was suggested that down-regulation of FSHR in the oviduct ampulla after estrus induction may be helpful for fertilization and early embryo development.

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Placental development during early pregnancy in sheep: Progesterone and estrogen receptor protein expression

Cited by 20 publications

References 67 publications

Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau†

Endometrial luminal epithelial cells sense embryo elongation in the roe deer independent of interferon-tau†

Placental development during early pregnancy in sheep: nuclear estrogen and progesterone receptor mRNA expression in the utero-placental compartments

Koyunlarda Östrus İndüksiyonu Sonrası Ovaryum, Oviduct ve Endometriyumda Folikül Uyarıcı Hormon, Luteotropik Hormon, Östrojen ve Progesteron Reseptör Ekspresyonu

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