Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo

Ding, Shih‐Torng; McNeel, Ronald L.; Mersmann, Harry J.

doi:10.1016/s0305-0491(99)00077-2

Cited by 92 publications

(67 citation statements)

References 38 publications

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“…As previously reported for human, mouse, and rat tissue distribution studies (Tontonoz et al 1993, Shimomura et al 1997, Kim et al 1998a, we were able to detect ADD1 transcript in pig liver, kidney, lung, ovary, skeletal muscle, adipose tissues, heart, and brain. Our results are in contrast with those of Ding et al (1999Ding et al ( , 2000, who reported ADD1 transcripts to be undetectable in pig kidney and skeletal muscle. In the current study, we used an adult sow to perform tissue distribution analysis, whereas Ding et al (1999Ding et al ( , 2000 used much younger pigs (20-30 kg).…”

Section: Discussioncontrasting

confidence: 99%

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Lord

Murphy²,

Desmarais³

et al. 2006

Reproduction

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Recent evidence points to a role for peroxisome proliferator-activated receptors (PPARs) d and g in embryo implantation and survival. In this study, we report the porcine PPARd complete coding sequence and mRNA abundance of PPARd, PPARg1 and g2, angiopoietin-like protein 4 (ANGPTL4) and adipocyte determination and differentiation-dependent factor 1 (ADD1) genes in the pregnant sow endometrium. Real-time PCR analysis was used to study the effect of parity (Yorkshire-Landrace multiparous (YL) and nulliparous (YLn)), site of endometrial tissue sampling (between and at embryo attachment sites) in crossbred Duroc!Yorkshire-Landrace (DYL) sows and stages of pregnancy (non-pregnant, day 15 and day 25 after mating) in Meishan-Landrace (ML) on mRNA levels. Parity effects were observed for PPARd, ANGPTL4, and ADD1, with higher mRNA levels in YL than YLn sows. In DYL sows, lower mRNA levels were present at attachment sites compared to between attachment sites for PPARd, PPARg1, and ANGPTL4. Finally, day 15 pregnant ML sows had lower PPARd mRNA levels compared to day 15 cycling ML sows. A significant increase of PPARg1 mRNA levels was found on day 25 pregnant ML and DYL sows relative to day 15 ML or DYL pregnant sows. PPARd and g immunostaining was detected in endometrial tissue of day 15 cycling sows, day 15 and 25 pregnant sows and epithelial cells of day 25 embryos. Collectively, our results suggest a role for PPARd, PPARg1, and ANGPTL4, but not PPARg2, during the peri-implantation period in pregnant sows. Reproduction (2006) 131 929-942

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Section: Discussioncontrasting

confidence: 99%

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Lord

Murphy²,

Desmarais³

et al. 2006

Reproduction

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“…This is the first global study of porcine adipose tissue regulatory factors since the most ambitious of previous studies of porcine adipose tissue regulatory factors were limited to fewer than 10 genes (Ding et al, 1999;. In this study we report, for the first time, gene expression of 16 nonsecreted regulatory factors in subcutaneous adipose tissue from growing pigs.…”

Section: Discussionmentioning

confidence: 90%

Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Hausman

Barb

Dean

2011

Animal

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The expression of many genes encoding secreted and non-secreted factors have been studied in human and rodent adipose tissue with cDNA microarrays, but few such studies in adipose tissue from growing pigs have been reported. Total RNA was collected at slaughter from outer subcutaneous adipose tissue (OSQ) and middle subcutaneous adipose tissue (MSQ) samples from gilts at 90, 150 and 210 days (n 5 5/age). Dye-labeled cDNA probes were hybridized to custom microarrays (70-mer oligonucleotides) representing about 600 pig genes involved in growth and reproduction. Gene expression intensity ratios changed little with age for 100 transcription factors, nuclear receptors, enzymes and other regulatory proteins in OSQ and MSQ from pigs between 90 and 210 days of age. However, the relative expression of 13 genes distinguished OSQ and MSQ depots in growing pigs. The expression of several genes were influenced by age including an increase in CCND3, HSF1 and PTGR1 expression in MSQ and a decrease in UCP2 and REA (prohibitin-2) expression in OSQ. These studies demonstrate for the first time the expression of several key regulatory genes in pig adipose tissue. Simple linear regression analysis showed that leptin gene expression was associated with expression of some of these regulatory genes. Negative associations between expression of some regulatory factors and leptin gene expression indicated that local leptin may decrease or antagonize adipogenesis.

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“…In mice, it was also detected abundantly in the tissues of lung, spleen and muscles besides liver and kidney, but it had a low expression level in paranephros, brain, heart, intestine and testis [8]. In swine, Ding et al [9] found the RXR gene abundantly expressed in liver and perirenal adipose tissue. There was only one report about expression profile of the cattle embryo [10], however, the integrated expression profile of bovine RXR gene is still vacant so far.…”

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confidence: 99%

Tissue expression, association analysis between three novel SNPs of the RXRα gene and growth traits in Chinese indigenous cattle

Gao

Feng

et al. 2013

Chin. Sci. Bull.

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Retinoid X receptor (RXR)  is a member of the nuclear hormone receptor superfamily that mediates the biological effects on several hormones, vitamins, and regulates lipid, glucose and energy metabolism. In this study, the tissue expression profiles of the bovine RXR gene and association analysis of single nucleotide polymorphisms (SNPs) with growth traits were carried out in 413 Chinese native cattle. The expression profile was analysed in ten Jiaxian cattle tissues by real-time PCR, and the results showed that RXR gene was abundantly expressed in adipose tissue and spleen, moderately expressed in heart, liver, lung, kidney, muscle and testis. Meanwhile, three SNPs (T27919A, T28139C and G28142A) and five haplotypes were identified. Haplotype with TTG was dominant with frequency of 69.1%. Chi-square test showed all populations were in Hardy-Weinberg equilibrium (P>0.05) at the three sites except Jiaxian cattle at G28142A site and Qinchuan cattle at T27919A site. Statistical analysis of combined sites showed that the individuals with TTGA genotype had significantly higher heart girth than those with TAGG genotype (P<0.05) and the animals with AAGA genotype had higher body weight than those with TAGG genotype (P<0.05) in T27919A-G28142A site. Heart girth, abdominal circumference and body weight of individuals with TCAG genotype were exceedingly higher than those with TTGG (P<0.01), TTGA and TCGG (P<0.05) in T28139C-G28142A site. For T27919A-T28139C site, the individuals of TCTA and TCTT genotype had significantly higher heart girth and lower height at hip cross than those with TTTA (P<0.05), and the body weight of TCAA and TCTT genotype individuals was higher than those with TTTA (P<0.05). In conclusion, these results provided evidence that the polymorphisms of RXR gene were associated with growth traits and might apply to Chinese indigenous yellow cattle breeding program as a possible candidate for marker-assisted selection (MAS).

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Expression of porcine adipocyte transcripts: tissue distribution and differentiation in vitro and in vivo

Cited by 92 publications

References 38 publications

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Modulation of peroxisome proliferator-activated receptor δ and γ transcripts in swine endometrial tissue during early gestation

Gene expression profiling in developing pig adipose tissue: non-secreted regulatory proteins

Tissue expression, association analysis between three novel SNPs of the RXRα gene and growth traits in Chinese indigenous cattle

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