Tissue expression, association analysis between three novel SNPs of the RXRα gene and growth traits in Chinese indigenous cattle

Abstract

Retinoid X receptor (RXR)  is a member of the nuclear hormone receptor superfamily that mediates the biological effects on several hormones, vitamins, and regulates lipid, glucose and energy metabolism. In this study, the tissue expression profiles of the bovine RXR gene and association analysis of single nucleotide polymorphisms (SNPs) with growth traits were carried out in 413 Chinese native cattle. The expression profile was analysed in ten Jiaxian cattle tissues by real-time PCR, and the results showed that RXR gene was abundantly expressed in adipose tissue and spleen, moderately expressed in heart, liver, lung, kidney, muscle and testis. Meanwhile, three SNPs (T27919A, T28139C and G28142A) and five haplotypes were identified. Haplotype with TTG was dominant with frequency of 69.1%. Chi-square test showed all populations were in Hardy-Weinberg equilibrium (P>0.05) at the three sites except Jiaxian cattle at G28142A site and Qinchuan cattle at T27919A site. Statistical analysis of combined sites showed that the individuals with TTGA genotype had significantly higher heart girth than those with TAGG genotype (P<0.05) and the animals with AAGA genotype had higher body weight than those with TAGG genotype (P<0.05) in T27919A-G28142A site. Heart girth, abdominal circumference and body weight of individuals with TCAG genotype were exceedingly higher than those with TTGG (P<0.01), TTGA and TCGG (P<0.05) in T28139C-G28142A site. For T27919A-T28139C site, the individuals of TCTA and TCTT genotype had significantly higher heart girth and lower height at hip cross than those with TTTA (P<0.05), and the body weight of TCAA and TCTT genotype individuals was higher than those with TTTA (P<0.05). In conclusion, these results provided evidence that the polymorphisms of RXR gene were associated with growth traits and might apply to Chinese indigenous yellow cattle breeding program as a possible candidate for marker-assisted selection (MAS).

Bovine HSD17B8 gene and its relationship with growth and meat quality traits

“…Figure 1 shows that the HSD17B8 was mainly expressed in the salpinx, liver, and kidney. This result was similar to those observed in humans and mice [1,11]. HSD17B8 expression in reproductive tissues plays a key role in maintaining sex steroid levels [2], which is important in controlling animal growth, reproduction, and lipid metabolism [12], and it can further affect meat quality.…”

The Duck RXRA Gene Promotes Adipogenesis and Correlates with Feed Efficiency

“…RXRA, a member of the nuclear hormone receptor superfamily, is involved in regulating lipid, glucose, and energy metabolism [23][24][25]. Fat metabolism is an important factor affecting the growth traits of meat ducks.…”

“…The duck RXRA gene is located on chromosome 18 between 6.20 and 6.34 Mb [37]. The RXRA gene of cattle is located in 105.98-106.01 Mb of chromosome 1, and its QTLs are related to growth performance and meat quality [23]. The RXRA gene of pigs is located at 288.83-288.86 Mb on chromosome 1, and its QTLs are correlated with abdominal fat, back fat, body weight, daily gain, and meat quality [38][39][40].…”

“…In addition, g.5,952,667 C > T causes the 40th amino acid (alanine) of the RXRA gene sequence to change to valine. Ala improves glucose metabolism to increase body energy [23]. Valine can promote PPARA-dependent fatty acid oxidation and the de novo synthesis of Pr-CoA fatty acids [45].…”

Tissue expression and predicted protein structures of the bovine ANGPTL3 and association of novel SNPs with growth and meat quality traits

“…Population genetic indices, such as heterozygosity (H e ), homozygosity (H o ), effective allele numbers (N e ) and polymorphism information content (PIC) were calculated according to Nei's methods (Nei and Roychoudhury, 1974;Ma et al, 2013). The formulas were as follows:…”