Expression of plasmid encoded Escherichia coli 5 S ribosomal ribonucleic acid in Pseudomonas putida

Abstract

The recombinant plasmid pNRK 36, which represents the plasmids RSF 1010, a small multicopy plasmid of the incompatability group IncQ that confers resistance to streptomycin and sulfoamide to its host cells, and pKK 223-3, which contains the try-lac (tat) promoter followed by a polylinker and a DNA segment containing the 5 S rRNA (rrn B) with the ribosomal RNA transcription terminators, was employed to transform Pseudomonas putida 2440 cells. The plasmid encoded 5 S rRNA from Escherichia coli was transcribed and processed properly in P. putida cells thus demonstrating for the first time the expression of a plasmid encoded ribosomal RNA in a heterologous system. The fact that the E. coli 5 S rRNA was not incorporated into assembled ribosomes suggests that the in vivo incorporation of 5 S rRNA into the 50 S ribosomal subunit is closely linked to 23 S rRNA and/or ribosomal protein synthesis. E. coli 5 S rRNAPlasmid encoding

Cloning and Expression in Escherichia coli of Proteus vulgaris Genes for 16S Ribosomal RNA

“…However, a successful in vivo expression of a plasmid-coded E. coli 5s rRNA gene in Pseudomonasputida was only reported recently (Hartmann et al, 1985).…”