An archaea 5S rRNA analog is stably expressed in Escherichia coli

Yang, Yanling; Fox, George E.

doi:10.1016/0378-1119(95)00683-4

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…Recently, biotechnology, such as gene probes (Yang and Fox 1996;Matheson et al 1997), PCR (Amici et al 1991;Khan et al 1998), monoclonal antibodies (RamosGonzalez et al 1992), bioluminescence (Heller et al 1992;Ripp et al 2000) and autofluorescent proteins (AFPs) (Gory et al 2001;Gather and Yun 2011;Ravikumar et al 2012), has been used to monitor GEMs. Of these, AFPs are the only technology that does not need additional substrates, cofactors, or proteins (Larrainzar et al 2005).…”

Section: Introductionmentioning

confidence: 99%

A safety type genetically engineered bacterium with red fluorescence which can be used to degrade organophosphorus pesticides

2013

Int. J. Environ. Sci. Technol.

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To effectively biodegrade organophosphorus pesticides residue in environment, we constructed a genetically engineered bacterium (GEB) which can not only emit red fluorescence but also degrade organophosphorus pesticides residue, and this GEB can commit suicide when required. Two genes with different functions were placed under the control of different promoters. One was the dual gene expression vector pL-DsRed-pL-OPH in which genes coding for DsRed and organophosphorus hydrolase were independently placed downstream of two pL promoters. These genes could be expressed freely as long as the GEB was alive. The other was the conditional suicide plasmid pDS containing two suicide cassettes designed to induce bacteria to commit suicide when they detect arabinose. The lethal gene used in the suicide plasmid was the nuclease gene of Serratia marcescens without the leader-coding sequence. This was put under the control of the T7 promoter. Applying this type of secure GEB could potentially be a less hazardous environmental strategy in degrading pesticides and contamination.

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Section: Introductionmentioning

confidence: 99%

A safety type genetically engineered bacterium with red fluorescence which can be used to degrade organophosphorus pesticides

2013

Int. J. Environ. Sci. Technol.

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Construction of genetically engineered bacteria that degrades organophosphorus pesticide residues and can be easily detected by the fluorescence

Wang

Chen

et al. 2013

Environmental Technology

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Organophosphorus compounds (OPs) are widely used in agriculture and industry and there is increased concern about their toxicological effects in the environment. Bioremediation can offer an efficient and cost-effective option for the removal of OPs. Herein, we describe the construction of a genetically engineered microorganism (GEM) that can degrade OPs and be directly detected and monitored in the environment using an enhanced green fluorescent protein (EGFP) fusion strategy. The coding regions of EGFP, a reporter protein that can fluoresce by itself, and organophosphorus hydrolase (OPH), which has a broad substrate specificity and is able to hydrolyse a number of organophosphorus pesticides, were cloned into the expression vector pET-28b. The fusion protein of EGFP-OPH was expressed in E. coli BL21 (DE3) and the protein expression reached the highest level at 11 h after isopropyl beta-D-thiogalactopyranoside induction. The fluorescence of the GEM was detected by fluorescence spectrophotometry and microscopy, and its ability to degrade OPs was determined by OPH activity assay. Those GEM that express the fusion protein (EGFP and OPH) exhibited strong fluorescence intensity and also potent hydrolase activity, which could be used to degrade organophosphorus pesticide residues in the environment and can also be directly monitored by fluorescence.

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An archaea 5S rRNA analog is stably expressed in Escherichia coli

Cited by 2 publications

References 24 publications

A safety type genetically engineered bacterium with red fluorescence which can be used to degrade organophosphorus pesticides

A safety type genetically engineered bacterium with red fluorescence which can be used to degrade organophosphorus pesticides

Construction of genetically engineered bacteria that degrades organophosphorus pesticide residues and can be easily detected by the fluorescence

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